ID Q6L2Y8_PICTO Unreviewed; 576 AA.
AC Q6L2Y8;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=PTO0078 {ECO:0000313|EMBL:AAT42663.1};
OS Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC
OS 100828 / KAW 2/3).
OC Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC Picrophilaceae; Picrophilus.
OX NCBI_TaxID=1122961 {ECO:0000313|EMBL:AAT42663.1, ECO:0000313|Proteomes:UP000000438};
RN [1] {ECO:0000313|EMBL:AAT42663.1, ECO:0000313|Proteomes:UP000000438}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828
RC {ECO:0000313|Proteomes:UP000000438};
RX PubMed=15184674; DOI=10.1073/pnas.0401356101;
RA Futterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C.,
RA Schepers B., Dock C., Antranikian G., Liebl W.;
RT "Genome sequence of Picrophilus torridus and its implications for life
RT around pH 0.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; AE017261; AAT42663.1; -; Genomic_DNA.
DR RefSeq; WP_011176879.1; NC_005877.1.
DR AlphaFoldDB; Q6L2Y8; -.
DR SMR; Q6L2Y8; -.
DR STRING; 263820.PTO0078; -.
DR REBASE; 8655; M.PtoORF78P.
DR PaxDb; 263820-PTO0078; -.
DR GeneID; 2845238; -.
DR KEGG; pto:PTO0078; -.
DR PATRIC; fig|263820.9.peg.92; -.
DR eggNOG; arCOG02632; Archaea.
DR HOGENOM; CLU_013049_4_2_2; -.
DR InParanoid; Q6L2Y8; -.
DR Proteomes; UP000000438; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000313|EMBL:AAT42663.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 91..222
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 231..540
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 544..571
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 576 AA; 67187 MW; 6F01B62860E83AC3 CRC64;
MADLNWIINR YNTMLSNFND RQFSRDEVIK LLKEKYNDNE GTVDTLISEM KKQELISSYQ
NEYDKRSVLY KLNKDFGLLK YTNNKITRSD LESMLKKAAD LIRTRVDYKY ILILLFLKRI
SDKWEEEYNK ALNELVDNGL NESEAKEEAK DEIYHEFNLP EDALWNNIRK DVNTLPEKLA
RALKTIAEMN PDLKNVIDNI DFMTFTTNSE NSQILRQLVE LFSEQELNNV SPDILGDAYE
WILRYFLPQK AKEGEIYTPR EVIKLLMNLL DPKPGDYIYD PACGTAGMLI TAYYYVKDKY
GKDYANKLFL YGQEANTTIY AVSKMNLYIH GIDDTNLSSG DTLLHPKNID ENKFDIVVAN
PPWNQDGYDE NVLKTGEYLN RYKYGFTNSS SADWAWIQHM LYTSKSKVGI ILDTGSLFRS
GKELAIRSKI IDNDFVESVI LLPEKIFYNT GSPSVIIILN KNKKIRNKIL FIDASKEFIK
HPEIRKLNTL SDDNINKITE AYKQFKNIDN FASVVDLNKI KENNYDLNIP LYIDNKENND
KIDIKNVLSE IKDLDLKLDN VNKKLDDYLM ELGYYE
//
