ID DCA13_XENTR Reviewed; 445 AA.
AC Q6NVS5;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=DDB1- and CUL4-associated factor 13;
DE AltName: Full=WD repeat and SOF domain-containing protein 1;
GN Name=dcaf13; Synonyms=wdsof1; ORFNames=TEgg103a13.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Egg;
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the small subunit (SSU) processome, first precursor
CC of the small eukaryotic ribosomal subunit. During the assembly of the
CC SSU processome in the nucleolus, many ribosome biogenesis factors, an
CC RNA chaperone and ribosomal proteins associate with the nascent pre-
CC rRNA and work in concert to generate RNA folding, modifications,
CC rearrangements and cleavage as well as targeted degradation of pre-
CC ribosomal RNA by the RNA exosome. {ECO:0000250|UniProtKB:Q9NV06}.
CC -!- FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3
CC ubiquitin-protein ligase complex. {ECO:0000250|UniProtKB:Q9NV06}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of the small subunit (SSU) processome, composed of more
CC than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.
CC Component of the DCX(DCAF13) E3 ubiquitin ligase complex, at least
CC composed of CUL4 (CUL4A or CUL4B), DDB1, DCAF13 and RBX1.
CC {ECO:0000250|UniProtKB:Q9NV06}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q9NV06}. Note=In the nucleolus, localizes
CC predominantly in the granular component, but also detected in the
CC fibrillar center and dense fibrillar component.
CC {ECO:0000250|UniProtKB:Q9NV06}.
CC -!- SIMILARITY: Belongs to the WD repeat DCAF13/WDSOF1 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR855579; CAJ83694.1; -; mRNA.
DR EMBL; BC067929; AAH67929.1; -; mRNA.
DR RefSeq; NP_998858.1; NM_213693.1.
DR AlphaFoldDB; Q6NVS5; -.
DR SMR; Q6NVS5; -.
DR STRING; 8364.ENSXETP00000018218; -.
DR PaxDb; 8364-ENSXETP00000008850; -.
DR Ensembl; ENSXETT00000100117; ENSXETP00000090636; ENSXETG00000004092.
DR GeneID; 407967; -.
DR KEGG; xtr:407967; -.
DR AGR; Xenbase:XB-GENE-5791289; -.
DR CTD; 25879; -.
DR Xenbase; XB-GENE-5791289; dcaf13.
DR eggNOG; KOG0268; Eukaryota.
DR InParanoid; Q6NVS5; -.
DR OMA; EDHNAYI; -.
DR OrthoDB; 5472351at2759; -.
DR Reactome; R-XTR-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR Reactome; R-XTR-8951664; Neddylation.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000008143; Chromosome 6.
DR Bgee; ENSXETG00000004092; Expressed in blastula and 13 other cell types or tissues.
DR ExpressionAtlas; Q6NVS5; differential.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:1990904; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR GO; GO:0001555; P:oocyte growth; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR CDD; cd00200; WD40; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR InterPro; IPR007287; Sof1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR22851:SF0; DDB1- AND CUL4-ASSOCIATED FACTOR 13; 1.
DR PANTHER; PTHR22851; U3 SMALL NUCLEOLAR RNA U3 SNORNA ASSOCIATED PROTEIN; 1.
DR Pfam; PF04158; Sof1; 1.
DR Pfam; PF00400; WD40; 4.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome; Repeat; Ribonucleoprotein;
KW Ribosome biogenesis; rRNA processing; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..445
FT /note="DDB1- and CUL4-associated factor 13"
FT /id="PRO_0000310434"
FT REPEAT 64..104
FT /note="WD 1"
FT REPEAT 107..146
FT /note="WD 2"
FT REPEAT 154..191
FT /note="WD 3"
FT REPEAT 194..234
FT /note="WD 4"
FT REPEAT 236..276
FT /note="WD 5"
FT REPEAT 280..319
FT /note="WD 6"
FT REPEAT 323..362
FT /note="WD 7"
FT REGION 353..441
FT /note="Required for nucleolar location"
FT /evidence="ECO:0000250|UniProtKB:Q6PAC3"
FT REGION 413..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 424..445
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 445 AA; 51302 MW; 32631BCC4C414E95 CRC64;
MKVKVLCRNP DDYVRETKRD LQRVPRNYDP ALHPFEVPRE YTRALNATKL ERVFAKPFIA
SLDGHRDGVN CIAKHPKSLS TVLSGACDGE VKIWNLTKRE CSRTIQAHDG FVRGLCVRFC
GTSFFTVGDD KTVKQWSMES PGYGEKVEPI RTILGKTVFT GIDHHMNDAI FATCGQQVDI
WDEQRSAPMR SYAWGVDSIS SIKFNPIETH ILSSCGTDRS IVLYDKRKPT PLKKIILEMR
TNTLCWNPME AFIFTAANEN FNLYTYDMRY MDGPVKVHMD HVSAVLDVDY SPTGKEFVSA
SFDKSIRIFP VQSGHSREVY HTKRMQHVTC VRWSADNKYV LCGSDEMNIR IWKANASEKL
GLLSPRERAA QNYNQKLKEK FHHHPQIKRI ARHRHLPRSI YSQIKEQQIM REARRKKDVN
RRKHSKPGSV PIPSEKKKHV LAVVE
//
