ID Q6NWG7_DANRE Unreviewed; 227 AA.
AC Q6NWG7; F6NNJ9; Q6PBI9;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 129.
DE RecName: Full=Tetraspanin {ECO:0000256|RuleBase:RU361218};
GN Name=cd9a {ECO:0000313|Ensembl:ENSDARP00000019805,
GN ECO:0000313|ZFIN:ZDB-GENE-030131-1175};
GN Synonyms=cd9 {ECO:0000313|EMBL:AAH67597.1,
GN ECO:0000313|RefSeq:NP_997784.1}, wu:fb61g04
GN {ECO:0000313|RefSeq:NP_997784.1}, zgc:73383
GN {ECO:0000313|RefSeq:NP_997784.1}, zgc:85743
GN {ECO:0000313|RefSeq:NP_997784.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH59691.1};
RN [1] {ECO:0000313|EMBL:AAH59691.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Wild-type {ECO:0000313|EMBL:AAH59691.1};
RC TISSUE=Eye {ECO:0000313|EMBL:AAH59691.1}, and Kidney
RC {ECO:0000313|EMBL:AAH67597.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|RefSeq:NP_997784.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=18850331;
RA Wang H.;
RT "Comparative genomic analysis of teleost fish bmal genes.";
RL Genetica 136:149-161(2009).
RN [3] {ECO:0000313|RefSeq:NP_997784.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=21035359;
RA Carrillo M., Kim S., Rajpurohit S.K., Kulkarni V., Jagadeeswaran P.;
RT "Zebrafish von Willebrand factor.";
RL Blood Cells Mol. Dis. 45:326-333(2010).
RN [4] {ECO:0000313|RefSeq:NP_997784.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=20875106;
RA Encinas P., Rodriguez-Milla M.A., Novoa B., Estepa A., Figueras A.,
RA Coll J.;
RT "Zebrafish fin immune responses during high mortality infections with viral
RT haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic
RT approach.";
RL BMC Genomics 11:518-518(2010).
RN [5] {ECO:0000313|RefSeq:NP_997784.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=22805612;
RA Yang D., Liu Q., Yang M., Wu H., Wang Q., Xiao J., Zhang Y.;
RT "RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway
RT and an inhibited MHC-II pathway at the early stage of vaccine immunization
RT in zebrafish.";
RL BMC Genomics 13:319-319(2012).
RN [6] {ECO:0000313|Ensembl:ENSDARP00000019805}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000019805};
RG Ensembl;
RL Submitted (FEB-2012) to UniProtKB.
RN [7] {ECO:0000313|Ensembl:ENSDARP00000019805, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000019805};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [8] {ECO:0000313|RefSeq:NP_997784.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=24683187;
RA Briolat V., Jouneau L., Carvalho R., Palha N., Langevin C., Herbomel P.,
RA Schwartz O., Spaink H.P., Levraud J.P., Boudinot P.;
RT "Contrasted innate responses to two viruses in zebrafish: insights into the
RT ancestral repertoire of vertebrate IFN-stimulated genes.";
RL J. Immunol. 192:4328-4341(2014).
RN [9] {ECO:0000313|RefSeq:NP_997784.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26469318;
RA Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M.,
RA Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D.,
RA Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E.,
RA Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M.,
RA Reith W., Hertzano R.;
RT "RFX transcription factors are essential for hearing in mice.";
RL Nat. Commun. 6:8549-8549(2015).
RN [10] {ECO:0000313|RefSeq:NP_997784.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27189481;
RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I.,
RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y.,
RA Bobe J.;
RT "Gene evolution and gene expression after whole genome duplication in fish:
RT the PhyloFish database.";
RL BMC Genomics 17:368-368(2016).
RN [11] {ECO:0000313|RefSeq:NP_997784.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141, ECO:0000256|RuleBase:RU361218}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141,
CC ECO:0000256|RuleBase:RU361218}. Secreted, extracellular exosome
CC {ECO:0000256|ARBA:ARBA00004550}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family.
CC {ECO:0000256|ARBA:ARBA00006840, ECO:0000256|RuleBase:RU361218}.
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DR EMBL; CU633973; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC059691; AAH59691.1; -; mRNA.
DR EMBL; BC067597; AAH67597.1; -; mRNA.
DR RefSeq; NP_997784.1; NM_212619.1.
DR Ensembl; ENSDART00000019571; ENSDARP00000019805; ENSDARG00000005842.
DR Ensembl; ENSDART00000019571.7; ENSDARP00000019805.4; ENSDARG00000005842.8.
DR GeneID; 322455; -.
DR KEGG; dre:322455; -.
DR AGR; ZFIN:ZDB-GENE-030131-1175; -.
DR CTD; 322455; -.
DR ZFIN; ZDB-GENE-030131-1175; cd9a.
DR OrthoDB; 2964111at2759; -.
DR TreeFam; TF352895; -.
DR Proteomes; UP000000437; Chromosome 18.
DR Bgee; ENSDARG00000005842; Expressed in liver and 31 other cell types or tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR CDD; cd03152; CD9_LEL; 1.
DR Gene3D; 1.10.1450.10; Tetraspanin; 1.
DR InterPro; IPR042055; CD9_LEL.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR018503; Tetraspanin_CS.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; TETRASPANIN; 1.
DR PANTHER; PTHR19282:SF546; TETRASPANIN; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR PRINTS; PR00259; TMFOUR.
DR SUPFAM; SSF48652; Tetraspanin; 1.
DR PROSITE; PS00421; TM4_1; 1.
PE 1: Evidence at protein level;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Fertilization {ECO:0000256|ARBA:ARBA00023279};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU361218};
KW Palmitate {ECO:0000256|ARBA:ARBA00023139};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q6NWG7};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Transmembrane {ECO:0000256|RuleBase:RU361218};
KW Transmembrane helix {ECO:0000256|RuleBase:RU361218}.
FT TRANSMEM 12..37
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU361218"
FT TRANSMEM 57..80
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU361218"
FT TRANSMEM 87..109
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU361218"
FT TRANSMEM 194..220
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU361218"
SQ SEQUENCE 227 AA; 25117 MW; 26E5BC25F4E638B8 CRC64;
MGVEGCPKCI KYSMFLLNSV FWIAGTAVLA VGLWLRFDPK TKSLFEGENS PYVFYTGVYI
LIAAGALMMV VGFFGCCGAI QESPCMLGLF FFFLLVIFAV EVAAGIWGFS NQTKVTEDIT
TFYRQTYDTY QQSKQEALKK TLRLFQHGLN CCGPSGNMQE SLDETCPKKE GLDNLIIKSC
PDAIDEVFNS KLHIIGGVGI ATGVIMIFGM IFSMMLCCAI RKTREIV
//
