GenomeNet

Database: UniProt
Entry: Q6PFE7
LinkDB: Q6PFE7
Original site: Q6PFE7 
ID   TEFF1_MOUSE             Reviewed;         372 AA.
AC   Q6PFE7; A2AJN3; Q8BRP7; Q8C536; Q9JJS1;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   10-APR-2019, entry version 116.
DE   RecName: Full=Tomoregulin-1;
DE            Short=TR-1;
DE   AltName: Full=M7365;
DE   AltName: Full=Transmembrane protein with EGF-like and one follistatin-like domain;
DE   Flags: Precursor;
GN   Name=Tmeff1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S.,
RA   She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W.,
RA   Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T.,
RA   Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J.,
RA   Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z.,
RA   Lindblad-Toh K., Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of
RT   the mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 28-372 (ISOFORM 2), TISSUE SPECIFICITY,
RP   AND DEVELOPMENTAL STAGE.
RC   TISSUE=Brain;
RX   PubMed=11025219; DOI=10.1016/S0925-4773(00)00426-3;
RA   Eib D.W., Holling T.M., Zwijsen A., Dewulf N., de Groot E.,
RA   van den Eijnden-van Raaij A.J.M., Huylebroeck D., Martens G.J.M.;
RT   "Expression of the follistatin/EGF-containing transmembrane protein
RT   M7365 (tomoregulin-1) during mouse development.";
RL   Mech. Dev. 97:167-171(2000).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 118-372 (ISOFORM 2), AND
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 125-372 (ISOFORM 3).
RC   STRAIN=C57BL/6J; TISSUE=Brain cortex, and Spinal cord;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [5]
RP   TISSUE SPECIFICITY.
RX   PubMed=11165370; DOI=10.1016/S0169-328X(00)00257-6;
RA   Kanemoto N., Horie M., Omori K., Nishino N., Kondo M., Noguchi K.,
RA   Tanigami A.;
RT   "Expression of TMEFF1 mRNA in the mouse central nervous system:
RT   precise examination and comparative studies of TMEFF1 and TMEFF2.";
RL   Brain Res. Mol. Brain Res. 86:48-55(2001).
RN   [6]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=11404089; DOI=10.1016/S0925-4773(01)00362-8;
RA   Morais da Silva S., Gates P.B., Eib D.W., Martens G.J.M.,
RA   Brockes J.P.;
RT   "The expression pattern of tomoregulin-1 in urodele limb regeneration
RT   and mouse limb development.";
RL   Mech. Dev. 104:125-128(2001).
CC   -!- FUNCTION: May inhibit NODAL and BMP signaling during neural
CC       patterning. {ECO:0000250}.
CC   -!- SUBUNIT: May interact with ST14. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass
CC       type I membrane protein {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q6PFE7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6PFE7-2; Sequence=VSP_024960;
CC       Name=3;
CC         IsoId=Q6PFE7-3; Sequence=VSP_024960, VSP_024961;
CC         Note=No experimental confirmation available.;
CC   -!- TISSUE SPECIFICITY: Expressed in brain, neurointermediate lobe,
CC       pars distalis, pancreas, ovary and testis.
CC       {ECO:0000269|PubMed:11025219, ECO:0000269|PubMed:11165370}.
CC   -!- DEVELOPMENTAL STAGE: At 8.5 dpc, highly expressed in the first
CC       branchial arch, somites, splanchnic mesoderm and ventral foregut
CC       epithelium. At 9.5 dpc, highly expressed in motor neurons and
CC       superficial neurons from the neural tube, and in the dorsal part
CC       of diencephalon and mesencephalon. At 11.5 dpc and 12.5 dpc,
CC       expressed in limbs. At 15.5 dpc, highly expressed in brain and
CC       spinal cord. {ECO:0000269|PubMed:11025219,
CC       ECO:0000269|PubMed:11404089}.
CC   -!- SIMILARITY: Belongs to the tomoregulin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB90827.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; AL807771; CAM15043.1; -; Genomic_DNA.
DR   EMBL; AL772151; CAM15043.1; JOINED; Genomic_DNA.
DR   EMBL; AL807771; CAM15044.1; -; Genomic_DNA.
DR   EMBL; AL772151; CAM15044.1; JOINED; Genomic_DNA.
DR   EMBL; AL772151; CAM20710.1; -; Genomic_DNA.
DR   EMBL; AL807771; CAM20710.1; JOINED; Genomic_DNA.
DR   EMBL; AL772151; CAM20711.1; -; Genomic_DNA.
DR   EMBL; AL807771; CAM20711.1; JOINED; Genomic_DNA.
DR   EMBL; BC057598; AAH57598.1; -; mRNA.
DR   EMBL; AJ400622; CAB90827.1; ALT_INIT; mRNA.
DR   EMBL; AK043792; BAC31655.1; -; mRNA.
DR   EMBL; AK079633; BAC37709.1; -; mRNA.
DR   CCDS; CCDS18168.1; -. [Q6PFE7-1]
DR   RefSeq; NP_067411.1; NM_021436.2. [Q6PFE7-1]
DR   RefSeq; XP_006537899.1; XM_006537836.1.
DR   RefSeq; XP_006537900.1; XM_006537837.1. [Q6PFE7-3]
DR   UniGene; Mm.422686; -.
DR   ProteinModelPortal; Q6PFE7; -.
DR   SMR; Q6PFE7; -.
DR   STRING; 10090.ENSMUSP00000030032; -.
DR   MEROPS; I01.974; -.
DR   iPTMnet; Q6PFE7; -.
DR   PhosphoSitePlus; Q6PFE7; -.
DR   PaxDb; Q6PFE7; -.
DR   PeptideAtlas; Q6PFE7; -.
DR   PRIDE; Q6PFE7; -.
DR   Ensembl; ENSMUST00000030032; ENSMUSP00000030032; ENSMUSG00000028347. [Q6PFE7-1]
DR   Ensembl; ENSMUST00000123476; ENSMUSP00000115841; ENSMUSG00000028347. [Q6PFE7-1]
DR   GeneID; 230157; -.
DR   KEGG; mmu:230157; -.
DR   UCSC; uc008svk.1; mouse. [Q6PFE7-1]
DR   UCSC; uc008svl.1; mouse. [Q6PFE7-2]
DR   CTD; 8577; -.
DR   MGI; MGI:1926810; Tmeff1.
DR   eggNOG; ENOG410KCWX; Eukaryota.
DR   eggNOG; ENOG410XQG4; LUCA.
DR   GeneTree; ENSGT00940000160714; -.
DR   HOGENOM; HOG000154541; -.
DR   HOVERGEN; HBG053816; -.
DR   InParanoid; Q6PFE7; -.
DR   OrthoDB; 773030at2759; -.
DR   PhylomeDB; Q6PFE7; -.
DR   TreeFam; TF330868; -.
DR   PRO; PR:Q6PFE7; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   Bgee; ENSMUSG00000028347; Expressed in 290 organ(s), highest expression level in embryo.
DR   ExpressionAtlas; Q6PFE7; baseline and differential.
DR   Genevisible; Q6PFE7; MM.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0007275; P:multicellular organism development; IEA:UniProtKB-KW.
DR   InterPro; IPR013032; EGF-like_CS.
DR   InterPro; IPR000742; EGF-like_dom.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   Pfam; PF07648; Kazal_2; 2.
DR   SMART; SM00280; KAZAL; 2.
DR   SUPFAM; SSF100895; SSF100895; 2.
DR   PROSITE; PS00022; EGF_1; 1.
DR   PROSITE; PS01186; EGF_2; 1.
DR   PROSITE; PS50026; EGF_3; 2.
DR   PROSITE; PS51465; KAZAL_2; 2.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Complete proteome;
KW   Developmental protein; Disulfide bond; EGF-like domain; Glycoprotein;
KW   Membrane; Reference proteome; Repeat; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL        1     35       {ECO:0000255}.
FT   CHAIN        36    372       Tomoregulin-1.
FT                                /FTId=PRO_0000286057.
FT   TOPO_DOM     36    322       Extracellular. {ECO:0000255}.
FT   TRANSMEM    323    343       Helical. {ECO:0000255}.
FT   TOPO_DOM    344    372       Cytoplasmic. {ECO:0000255}.
FT   DOMAIN       85    137       Kazal-like 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00798}.
FT   DOMAIN      176    229       Kazal-like 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00798}.
FT   DOMAIN      263    303       EGF-like. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00076}.
FT   COMPBIAS     42     45       Poly-Gly.
FT   CARBOHYD     55     55       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   DISULFID     91    121       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   DISULFID     95    114       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   DISULFID    103    135       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   DISULFID    182    213       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   DISULFID    186    206       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   DISULFID    195    227       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   DISULFID    267    280       {ECO:0000250}.
FT   DISULFID    275    291       {ECO:0000250}.
FT   DISULFID    293    302       {ECO:0000250}.
FT   VAR_SEQ     146    146       G -> GA (in isoform 2 and isoform 3).
FT                                {ECO:0000303|PubMed:11025219,
FT                                ECO:0000303|PubMed:16141072}.
FT                                /FTId=VSP_024960.
FT   VAR_SEQ     251    252       Missing (in isoform 3).
FT                                {ECO:0000303|PubMed:16141072}.
FT                                /FTId=VSP_024961.
FT   CONFLICT    235    235       L -> S (in Ref. 4; CAB90827).
FT                                {ECO:0000305}.
FT   CONFLICT    241    241       D -> P (in Ref. 4; CAB90827).
FT                                {ECO:0000305}.
FT   CONFLICT    244    244       Q -> L (in Ref. 4; CAB90827).
FT                                {ECO:0000305}.
FT   CONFLICT    370    370       R -> K (in Ref. 4; CAB90827).
FT                                {ECO:0000305}.
SQ   SEQUENCE   372 AA;  40114 MW;  BB3DA6710B78A530 CRC64;
     MGAQAPLRLP AAPPLAVCGY TSVLLLFAFC LPGSRASNQP AGGGGDCPGG RGKSNCSELN
     LRESDIRVCD ESSCKYGGVC KEDGDGLKCA CQFQCHTNYI PVCGSNGDTY QNECFLRRAA
     CKHQKDITVV ARGPCYSDNG SGSGEGEEEG SGAGAHRKHS KCGPCKYKAE CDEDAENVGC
     VCNIDCSGYS FNPVCASDGS SYNNPCFVRE ASCIKQEQID IRHLGHCTDT DDVSLLGKKD
     DGLQYRPDVK DAGDEREDVY IGSHMPCPEN LNGYCIHGKC EFIYSTQKAS CRCESGYTGQ
     HCEKTDFSIL YVVPSRQKLT HVLIAAIIGA VQIAIIVAIV MCITRKCPKN NRGRRQKQNL
     GHFTSDTSSR MV
//
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