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Database: UniProt
Entry: Q70KF0
LinkDB: Q70KF0
Original site: Q70KF0 
ID   DOIAD_MICEC             Reviewed;         340 AA.
AC   Q70KF0;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   05-DEC-2018, entry version 69.
DE   RecName: Full=2-deoxy-scyllo-inosamine dehydrogenase;
DE            Short=DOIA dehydrogenase;
DE            EC=1.1.1.329;
GN   Name=gacH; Synonyms=genE, gntP;
OS   Micromonospora echinospora (Micromonospora purpurea).
OC   Bacteria; Actinobacteria; Micromonosporales; Micromonosporaceae;
OC   Micromonospora.
OX   NCBI_TaxID=1877;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15835 / DSM 43036 / BCRC 11561 / JCM 3074 / NBRC 12575 /
RC   NCIMB 12882 / NRRL 2953;
RX   PubMed=15376556; DOI=10.7164/antibiotics.57.436;
RA   Unwin J., Standage S., Alexander D., Hosted T. Jr., Horan A.C.,
RA   Wellington E.M.;
RT   "Gene cluster in Micromonospora echinospora ATCC15835 for the
RT   biosynthesis of the gentamicin C complex.";
RL   J. Antibiot. 57:436-445(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15835 / DSM 43036 / BCRC 11561 / JCM 3074 / NBRC 12575 /
RC   NCIMB 12882 / NRRL 2953;
RX   PubMed=15359126;
RA   Kharel M.K., Basnet D.B., Lee H.C., Liou K., Moon Y.H., Kim J.-J.,
RA   Woo J.S., Sohng J.K.;
RT   "Molecular cloning and characterization of a 2-deoxystreptamine
RT   biosynthetic gene cluster in gentamicin-producing Micromonospora
RT   echinospora ATCC15835.";
RL   Mol. Cells 18:71-78(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15835 / DSM 43036 / BCRC 11561 / JCM 3074 / NBRC 12575 /
RC   NCIMB 12882 / NRRL 2953;
RA   Aboshanab K.M.A., Schmidt-Beissner H., Wehmeier U.F., Welzel K.,
RA   Vente A., Piepersberg W.;
RT   "Cloning and sequencing of the gentamicin biosynthetic gene cluster
RT   from Micromonospora echinospora DSM 43036.";
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine
CC       (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo-
CC       inosose (amino-DOI). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-deoxy-scyllo-inosamine + NADP(+) = 3-amino-2,3-dideoxy-
CC         scyllo-inosose + H(+) + NADPH; Xref=Rhea:RHEA:33879,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:65002, ChEBI:CHEBI:65003; EC=1.1.1.329;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-deoxy-scyllo-inosamine + NAD(+) = 3-amino-2,3-dideoxy-
CC         scyllo-inosose + H(+) + NADH; Xref=Rhea:RHEA:33883,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:65002, ChEBI:CHEBI:65003; EC=1.1.1.329;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; 2-deoxystreptamine
CC       biosynthesis; 2-deoxystreptamine from D-glucose 6-phosphate: step
CC       3/4.
CC   -!- PATHWAY: Antibiotic biosynthesis; gentamicin biosynthesis.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. DOIA dehydrogenase subfamily. {ECO:0000305}.
DR   EMBL; AY524043; AAR98562.1; -; Genomic_DNA.
DR   EMBL; AJ575934; CAF34032.1; -; Genomic_DNA.
DR   EMBL; AJ628149; CAF31445.1; -; Genomic_DNA.
DR   ProteinModelPortal; Q70KF0; -.
DR   SMR; Q70KF0; -.
DR   UniPathway; UPA00907; UER00923.
DR   UniPathway; UPA00967; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR013149; ADH_C.
DR   InterPro; IPR013154; ADH_N.
DR   InterPro; IPR029752; D-isomer_DH_CS1.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Metal-binding; NAD; NADP; Oxidoreductase; Zinc.
FT   CHAIN         1    340       2-deoxy-scyllo-inosamine dehydrogenase.
FT                                /FTId=PRO_0000234042.
FT   METAL        41     41       Zinc 1; catalytic. {ECO:0000250}.
FT   METAL        63     63       Zinc 1; catalytic. {ECO:0000250}.
FT   METAL        92     92       Zinc 2. {ECO:0000250}.
FT   METAL        95     95       Zinc 2. {ECO:0000250}.
FT   METAL        98     98       Zinc 2. {ECO:0000250}.
FT   METAL       106    106       Zinc 2. {ECO:0000250}.
FT   METAL       147    147       Zinc 1; catalytic. {ECO:0000250}.
SQ   SEQUENCE   340 AA;  36023 MW;  63200F3BE27F3D09 CRC64;
     MGGRMRALQF HGGHKAQIID TPRPEAPPGW AVVKVHYCCL CGSDLWLYRG KWHGNRYPIV
     PGHEWAGVVD SAPEGYESWV GRPVTGDLIV GCQGCGPCRD GLPVMCENLI EIGFTVDGGC
     AGYVAVPITN LYLLPEGMDL AAASQTEPLA VALHAVDRIN LRPAERVAVL GAGGIGQLIL
     QSARATGATV TLATDLVAER RKIAEESGAA AAVHPSELPE LTSYADKVDV VFEASGDPES
     VVRALDLVRP GGRVCLVGYQ VGAEHALETA RLPLSYASLV GVMGPGGKYR EAVDLLANGA
     IDTQPILTDI VTLDDYAPAI DRAINRTDGT VRVVFDLRNE
//
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