GenomeNet

Database: UniProt
Entry: Q71TF8
LinkDB: Q71TF8
Original site: Q71TF8 
ID   DARB_BPP1               Reviewed;        2255 AA.
AC   Q71TF8;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   18-SEP-2019, entry version 73.
DE   RecName: Full=Defense against restriction protein B {ECO:0000303|PubMed:3029954};
DE            EC=3.6.4.- {ECO:0000305};
DE   AltName: Full=DarB {ECO:0000312|EMBL:AAQ14093.1};
GN   Name=darB {ECO:0000312|EMBL:AAQ14093.1};
OS   Escherichia phage P1 (Bacteriophage P1).
OC   Viruses; Caudovirales; Myoviridae; Punavirus.
OX   NCBI_TaxID=10678 {ECO:0000312|Proteomes:UP000008091};
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Mod1902::IS5 c1.100 rev dmt {ECO:0000312|EMBL:AAQ14093.1}, and
RC   Mod749::IS5 c1.100 mutant {ECO:0000312|EMBL:AAQ13985.1};
RX   PubMed=15489417; DOI=10.1128/jb.186.21.7032-7068.2004;
RA   Lobocka M.B., Rose D.J., Plunkett G. III, Rusin M., Samojedny A.,
RA   Lehnherr H., Yarmolinsky M.B., Blattner F.R.;
RT   "Genome of bacteriophage P1.";
RL   J. Bacteriol. 186:7032-7068(2004).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=3029954; DOI=10.1016/0042-6822(87)90324-2;
RA   Iida S., Streiff M.B., Bickle T.A., Arber W.;
RT   "Two DNA antirestriction systems of bacteriophage P1, darA, and darB:
RT   characterization of darA- phages.";
RL   Virology 157:156-166(1987).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=28509398; DOI=10.1111/mmi.13705;
RA   Piya D., Vara L., Russell W.K., Young R., Gill J.J.;
RT   "The multicomponent antirestriction system of phage P1 is linked to
RT   capsid morphogenesis.";
RL   Mol. Microbiol. 105:399-412(2017).
CC   -!- FUNCTION: Capsid internal protein that is probably ejected along
CC       with the viral DNA and prevents degradation of viral DNA by the
CC       host EcoB and EcoK restriction-modification antiviral defense
CC       systems. {ECO:0000269|PubMed:28509398,
CC       ECO:0000269|PubMed:3029954}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:28509398,
CC       ECO:0000269|PubMed:3029954}. Note=Internal capsid protein. DarA is
CC       required for DarB incorporation into the virions.
CC       {ECO:0000269|PubMed:28509398}.
CC   -!- MISCELLANEOUS: Phage P1 Dar antirestriction system is composed of
CC       Hdf, DarA, DdrA, DdrB, DarB and Ulx. Hdf and DarA are incorporated
CC       first in the procapsid in a co-dependent manner, followed by DarB,
CC       DdrB and Ulx. {ECO:0000269|PubMed:28509398}.
CC   -!- SIMILARITY: Belongs to the helicase family. {ECO:0000305}.
DR   EMBL; AF234172; AAQ13985.1; -; Genomic_DNA.
DR   EMBL; AF234173; AAQ14093.1; -; Genomic_DNA.
DR   RefSeq; YP_006479.1; NC_005856.1.
DR   GeneID; 2777481; -.
DR   KEGG; vg:2777481; -.
DR   Proteomes; UP000001577; Genome.
DR   Proteomes; UP000008091; Genome.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006306; P:DNA methylation; IEA:InterPro.
DR   GO; GO:0099018; P:restriction-modification system evasion by virus; IMP:UniProtKB.
DR   Gene3D; 3.40.50.10810; -; 2.
DR   InterPro; IPR003356; DNA_methylase_A-5.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF02384; N6_Mtase; 1.
DR   Pfam; PF00176; SNF2_N; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Coiled coil; Complete proteome; Helicase;
KW   Host-virus interaction; Hydrolase; Nucleotide-binding;
KW   Reference proteome; Restriction-modification system evasion by virus;
KW   Virion.
FT   CHAIN         1   2255       Defense against restriction protein B.
FT                                /FTId=PRO_0000433213.
FT   DOMAIN      841   1126       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN     1383   1568       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     854    861       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   COILED     1198   1234       {ECO:0000255}.
FT   COILED     1617   1654       {ECO:0000255}.
FT   MOTIF      1052   1055       DEAH box. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
SQ   SEQUENCE   2255 AA;  251543 MW;  383BAA4D9D28341F CRC64;
     MNKLSMGVFR CSSVSEILKY IRAITSHRAP IKYGVEKVEG KSYDRLRREA NQKAIDLLNS
     LVDGATLTDE QRQILAGYTG EGGIGGSVSE YYTPKPIAEG VWEIMKLYGA DVGNTLEPSA
     GTGVFNETKP VGTVMTATEI SSVSGRINQL LHPEDSVQIS PFEQLAVSTP NDSFDHVVGN
     VPFGGRDNTR NIDKPYAEET DMGSYFMLRM LDKIKPGGFM CVIVPPSIVS GSNMKRLRLR
     LSRKAEFLGA HRLPTGTFDA NGTSTVVDVV LMRKHPAEMA EKIPLVHEST LESANVLWPT
     FISGKWFEKD GRRFVHGTQE KGFQGRIEVR ADGQIDNQAL KAKLIHCFES RIDWYLLDMA
     EPSPTADVVD EGEMRLINGV WQKYAGGRWI ESDAGKELKI DVASYGADSW EALQRNLTTT
     EGRLGMTFTQ MANVRDKYTT SISDDMVQLV DWINSQPEKY RERLYRGAMI GRMLIEYQDM
     KAAGHSAEQI EQQRLSLVSR LQAEIDRFGN PGRGPIAKLS GSGARAWFAF RGAIKLDGTI
     SDELTGKLVT HDSSASYDST SYQDTLRYLY SDLTRDPIQL DDFRLAFTGE LPASDDELLN
     LLASTPGIAV SPYGGIVPFA RATSGDINEI VAPKQEFLAT LTDGPVKNNV LNQLAAIEEK
     RIKTPAENIR FKLNSRWFDR SVILEFLQEN GYPDLRYVQS VQLEGDEMVS DTYHGGDGLF
     VGHRYGVVQR KDKETGEIRY EWDRKSGENA TGFPAQLEKY LNGARIGGKD SATANGYREQ
     MALLEDQFNK WIKTHDRYDE LVAKYNDVFN SNIPYEHSGD PLGLKGLSGK RQPFDYQNSE
     VRRLSEDGRG ILGFGTGLGK TTTALALEAF NYENGRSTRT AYVVPKSVLE NWYYEAKEFL
     SEEAFSNYLF VGLDVLMDGD QIRQVPVLDE NGKPVLGTDG TPVMRDALKL ADEATITARM
     NAIPHSNYRA VVFTKEQYAR IPLRDDTVDE HAQDMLYDFV AAGRVASAMD SDSHRKEAAR
     RRVLSEYSDT GTEKAEKYPY FEDMGFDSVI ADEGHNYRNS YKNGREASQL AYLPTSAVAQ
     SARDMAIKNA YLMKKNGGRG PVLLTATPVV NTPIDAYNML SHVLPKEYWQ KMGIYGPDDF
     VKFFGKTRLE TVQKISGEVE EKMALVGFEN LDALRGIFHR WTTLKTAEDV KDTVEIPELD
     EHQQDAPLTE EQLAAYEELR QQAEAAAKAN NGVTTSVNED GVIEHEKARP IFSIIRDMDR
     VCTDMDLYYR RITYRFLPEY ADAVQQLADS LPKQATSEDD DSDDSITQQS QYSLIDKGEF
     IQLQVPEAFE QEVNKRLARF GIDEQTVTHP VTPKYAKLIA TLKEFFPEGK QIIFTDEKTQ
     HQKLKRIICN ALNLEPSKVG ILNAQTVAEA GKTGKKLKAV KPPKELPDEP TDAQIAKYNE
     QMALYDAYIA QQNEMSLGGL EKIAADFQEG RTPIIICNKK AEVGINLHRG TTDIHHLTLP
     WTPASIAQRN GRGARVGSNR ASVRVHYYCG KGSFDEYRLK TLKRKAGWIS DILRSDKSEM
     ENADANDMIE MQMYTAKDDG ERLAMMQVQM DKAKAAQRAR QKEQATIDLQ NYIKAQHAAG
     EDVEVLTAEL ERSKAELEKT TAEVAKFKQA VMAKAADNAD WKARWGSVHH TDRMLLAQYR
     ASLKSAIQRK ANISQAISPY EKLLNRTQKA ATDIKRLRPL VEDAINKGIL DVDPDLVNHA
     SEFLVIGDRS WRVGQYYDCA GDIVRIKSLD FDSQRADVEI IFTFKGTKSG NWDVKTLDKQ
     VDVTPDEDAV MQKISGGVSI AGINDIISCD DFYRFQQRGM IKITDSYGVQ TTESGYSIDF
     VGTYTDPLKH AVYPDRRDGA LKSSIAKWVL GMMSEGNNRQ VRLAEVFLTE LFGSNYGDVI
     ASYGDTLSPE AIQEKIADAI ARMPEKTSQG ATRNGDSELE VTNAIFGTHE FRASDYEITT
     AQFGTIGIYS NKAEIKQAMD AASARIAAER EANLNHAVAA LTQSWVTAIR EAATTGKITP
     AIADVVNDGS KFMDAYKMDA VQLPSAYGQL SYRMTYNLVS MFSDLAILGL VDLNEVTPEL
     LSMRKNHVEI LQRINTVLAG RTDEEKQADA DRINLALGNI TEEEIAARNE KQEELSSIQG
     DATSIAQSLG LNYRVSTADL KMMYAPKFAA GEVFGLQEAS GMKGVLFRAK DAIKTKFGAR
     WLPAKAKNSD FPGNWWIIET KHNVADVLAV IQQYA
//
DBGET integrated database retrieval system