ID Q74AF7_GEOSL Unreviewed; 473 AA.
AC Q74AF7;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 95.
DE SubName: Full=Methyl viologen-reducing hydrogenase, large subunit {ECO:0000313|EMBL:AAR35792.1};
GN Name=mvhL {ECO:0000313|EMBL:AAR35792.1};
GN OrderedLocusNames=GSU2419 {ECO:0000313|EMBL:AAR35792.1};
OS Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA).
OC Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales;
OC Geobacteraceae; Geobacter.
OX NCBI_TaxID=243231 {ECO:0000313|EMBL:AAR35792.1, ECO:0000313|Proteomes:UP000000577};
RN [1] {ECO:0000313|EMBL:AAR35792.1, ECO:0000313|Proteomes:UP000000577}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51573 / DSM 12127 / PCA
RC {ECO:0000313|Proteomes:UP000000577};
RX PubMed=14671304; DOI=10.1126/science.1088727;
RA Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.,
RA Heidelberg J.F., Wu D., Wu M., Ward N., Beanan M.J., Dodson R.J.,
RA Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.,
RA Kolonay J.F., Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M.,
RA Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.,
RA Khouri H., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R.,
RA Fraser C.M.;
RT "Genome of Geobacter sulfurreducens: metal reduction in subsurface
RT environments.";
RL Science 302:1967-1969(2003).
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
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DR EMBL; AE017180; AAR35792.1; -; Genomic_DNA.
DR RefSeq; NP_953465.1; NC_002939.5.
DR RefSeq; WP_010943056.1; NC_002939.5.
DR AlphaFoldDB; Q74AF7; -.
DR SMR; Q74AF7; -.
DR STRING; 243231.GSU2419; -.
DR EnsemblBacteria; AAR35792; AAR35792; GSU2419.
DR KEGG; gsu:GSU2419; -.
DR PATRIC; fig|243231.5.peg.2447; -.
DR eggNOG; COG3259; Bacteria.
DR HOGENOM; CLU_044556_0_0_7; -.
DR InParanoid; Q74AF7; -.
DR OrthoDB; 9761717at2; -.
DR Proteomes; UP000000577; Chromosome.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR43600; COENZYME F420 HYDROGENASE, SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43600:SF2; CYTOSOLIC NIFE-HYDROGENASE, ALPHA SUBUNIT; 1.
DR Pfam; PF00374; NiFeSe_Hases; 2.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|PIRSR:PIRSR601501-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000000577}.
FT BINDING 43
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 62
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 65
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 65
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 397
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 443
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 446
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 449
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 473 AA; 51321 MW; 920CDB75CE9AED74 CRC64;
MNKTITIDPV TRIEGHARVF IDLDDTGALT AAGLVVNELR GFEKILTGMD ADRMPLVTAR
ICGVCPTAHH LAATNALNNA AGVEPPPAAK LLRELMYMGH VIHSHCLSLF VLQGPDLVLG
LDADPAVRNV VGVVQAAPDI AKKALQCRSI GQKINELVGG RGTHPVTSVA GGIAFVLDAD
RERQLNAWIN EARDLVLELA PVVKQLLMKM LDTHPAMLQQ WVAPAWSVGT VKADGTLALV
NGEIRAVDEV GALKARFPSA DYDSYMSESA VEFSYMKQVS FVDGDNRHDY RVGPLARLNV
SERYGTPLAD REREEFIRTV GRPCHANALQ PWARLIELLY CTERAGEIMA DPAIHGETRV
PVTFSGGRGV GHVEAPRGTL IHDYEIDENG IVRAANLIVA TQQNYALINA MIAQAATSHV
INRPDDQALL NAVEFGIRCY DPCLSCATHA LGAMPLEIVV NRAAKPRVIR RNC
//
