ID   Q74JI3_LACJO            Unreviewed;       559 AA.
AC   Q74JI3;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 1.
DT   27-MAR-2024, entry version 121.
DE   SubName: Full=Acetolactate synthase {ECO:0000313|EMBL:AAS08946.1};
GN   OrderedLocusNames=LJ_1124 {ECO:0000313|EMBL:AAS08946.1};
OS   Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=257314 {ECO:0000313|EMBL:AAS08946.1, ECO:0000313|Proteomes:UP000000581};
RN   [1] {ECO:0000313|EMBL:AAS08946.1, ECO:0000313|Proteomes:UP000000581}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CNCM I-1225 / La1 / NCC 533
RC   {ECO:0000313|Proteomes:UP000000581};
RX   PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA   Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA   Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA   Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT   "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT   johnsonii NCC 533.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
CC   -!- SIMILARITY: Belongs to the TPP enzyme family.
CC       {ECO:0000256|ARBA:ARBA00007812, ECO:0000256|RuleBase:RU362132}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE017198; AAS08946.1; -; Genomic_DNA.
DR   RefSeq; WP_011161963.1; NC_005362.1.
DR   AlphaFoldDB; Q74JI3; -.
DR   GeneID; 83570439; -.
DR   KEGG; ljo:LJ_1124; -.
DR   PATRIC; fig|257314.6.peg.989; -.
DR   eggNOG; COG0028; Bacteria.
DR   HOGENOM; CLU_013748_3_2_9; -.
DR   Proteomes; UP000000581; Chromosome.
DR   GO; GO:0003984; F:acetolactate synthase activity; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR   GO; GO:0034077; P:butanediol metabolic process; IEA:InterPro.
DR   CDD; cd07035; TPP_PYR_POX_like; 1.
DR   Gene3D; 3.40.50.970; -; 2.
DR   Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR   InterPro; IPR012782; Acetolactate_synth_catblc.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR   InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR   InterPro; IPR000399; TPP-bd_CS.
DR   InterPro; IPR045229; TPP_enz.
DR   InterPro; IPR011766; TPP_enzyme_TPP-bd.
DR   NCBIfam; TIGR02418; acolac_catab; 1.
DR   PANTHER; PTHR18968:SF129; ACETOLACTATE SYNTHASE; 1.
DR   PANTHER; PTHR18968; THIAMINE PYROPHOSPHATE ENZYMES; 1.
DR   Pfam; PF02775; TPP_enzyme_C; 1.
DR   Pfam; PF00205; TPP_enzyme_M; 1.
DR   Pfam; PF02776; TPP_enzyme_N; 1.
DR   SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR   SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR   PROSITE; PS00187; TPP_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Thiamine pyrophosphate {ECO:0000256|RuleBase:RU362132}.
FT   DOMAIN          8..122
FT                   /note="Thiamine pyrophosphate enzyme N-terminal TPP-
FT                   binding"
FT                   /evidence="ECO:0000259|Pfam:PF02776"
FT   DOMAIN          201..329
FT                   /note="Thiamine pyrophosphate enzyme central"
FT                   /evidence="ECO:0000259|Pfam:PF00205"
FT   DOMAIN          393..539
FT                   /note="Thiamine pyrophosphate enzyme TPP-binding"
FT                   /evidence="ECO:0000259|Pfam:PF02775"
SQ   SEQUENCE   559 AA;  61683 MW;  B209199ED4909D11 CRC64;
     MAEEKYYGAN AIVDSLINHD VKFVFGIPGA KIDRVFELLE HSKDPRTPKF VLTRHEQNAA
     FMAAGVGRIT GKPGVVLTTS GPGASNLATG LVTATAEGDP VLAISGQVQR TDLLRLTHQS
     MKNAALFAPI TKYSAEVQEP ENISEVIANA YQEAEASKQG ASFVSVPQDV TDAEVKTSVI
     APLEPPKLGP ASPIESTLLS QRIKAAKLPV LLLGMRASDP ETTRAIRRLI AETHLPVVET
     FQGAGIIPRE LEDDFFGRVG LFRNQPGDQL LKKSDLVIAI GYDPIEYEPR NWNSDRSANI
     VVIDSMRAEI DKNYQPEREL VGDISQTLDF LLPYMKGYSM PNESKEYLDS LRKTLQKRDT
     PPAVTKDQVK SHPLSIIQAL QDRVTDDMTV TVDVGSFYIW MARHFRSYEP RHLLFSNGMQ
     TLGVALPWAV AAALVRPNTK IVSVSGDGGF MFSSQDLETA VRLGLNIVHI IWNDGNYDMV
     KFQEELKYGD SAAVKFGPVD FVKYAESFGA KGYRVEKAAD LDRILDKAFK EDGPTVVDIP
     VDYSFNQELG KTILEDQLH
//