ID Q74N74_NANEQ Unreviewed; 676 AA.
AC Q74N74;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN OrderedLocusNames=NEQ068 {ECO:0000313|EMBL:AAR38923.1};
OS Nanoarchaeum equitans (strain Kin4-M).
OC Archaea; Nanoarchaeota; Candidatus Nanoarchaeia; Nanoarchaeales;
OC Nanoarchaeaceae; Nanoarchaeum.
OX NCBI_TaxID=228908 {ECO:0000313|EMBL:AAR38923.1, ECO:0000313|Proteomes:UP000000578};
RN [1] {ECO:0000313|EMBL:AAR38923.1, ECO:0000313|Proteomes:UP000000578}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Kin4-M {ECO:0000313|EMBL:AAR38923.1,
RC ECO:0000313|Proteomes:UP000000578};
RX PubMed=14566062; DOI=10.1073/pnas.1735403100;
RA Waters E., Hohn M.J., Ahel I., Graham D.E., Adams M.D., Barnstead M.,
RA Beeson K.Y., Bibbs L., Bolanos R., Keller M., Kretz K., Lin X., Mathur E.,
RA Ni J., Podar M., Richardson T., Sutton G.G., Simon M., Soll D.,
RA Stetter K.O., Short J.M., Noordewier M.;
RT "The genome of Nanoarchaeum equitans: insights into early archaeal
RT evolution and derived parasitism.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:12984-12988(2003).
RN [2] {ECO:0007829|PDB:5OXW, ECO:0007829|PDB:5OXX}
RP X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 572-676.
RX PubMed=29754955; DOI=10.1016/j.chembiol.2018.04.008;
RA Gordo V., Aparicio D., Perez-Luque R., Benito A., Vilanova M., Uson I.,
RA Fita I., Ribo M.;
RT "Structural Insights into Subunits Assembly and the Oxyester Splicing
RT Mechanism of Neq pol Split Intein.";
RL Cell Chem. Biol. 25:871-879.e2(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755}.
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DR EMBL; AE017199; AAR38923.1; -; Genomic_DNA.
DR PDB; 5OXW; X-ray; 2.61 A; A/B/C/D=579-676.
DR PDB; 5OXX; X-ray; 1.74 A; A=572-676.
DR PDB; 5OXZ; X-ray; 1.20 A; A=572-676.
DR PDBsum; 5OXW; -.
DR PDBsum; 5OXX; -.
DR PDBsum; 5OXZ; -.
DR AlphaFoldDB; Q74N74; -.
DR SMR; Q74N74; -.
DR STRING; 228908.NEQ068; -.
DR EnsemblBacteria; AAR38923; AAR38923; NEQ068.
DR KEGG; neq:NEQ068; -.
DR PATRIC; fig|228908.8.peg.69; -.
DR HOGENOM; CLU_000203_6_0_2; -.
DR BioCyc; NEQU228908:GJB6-75-MONOMER; -.
DR Proteomes; UP000000578; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR Gene3D; 3.30.342.10; DNA Polymerase, chain B, domain 1; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10322; DNA POLYMERASE CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR10322:SF23; DNA POLYMERASE DELTA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:5OXW, ECO:0007829|PDB:5OXX};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000000578};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 76..310
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 381..586
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT COILED 491..518
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 676 AA; 79864 MW; DAC580CEE616863D CRC64;
MLHQLPTMVV EEKAVKEEEG YSVLKCYWIN IENTPLDEVI LIGKDENNRA CEVIIPYKWY
FYFEGDIKDL EEFANNKKIK IEYTKEQKKY IEKPKDVYKV YVLHKHYPIL KEFIKEKGYK
KYETDINVYR KFLIDKGIEP FEWFEVEGKI LLSTSNKVRI KAQSIKRLYE KTKPSVLAFD
IEVYSEAFPN PEKDKIISIA LYGDNYEGVI SYKGEPTIKV NTEYELIEKF VEIIESLKPD
IIVTYNGDNF DIDFLVKRAS LYNIRLPIKL VNKKEPTYNF RESAHVDLYK TITTIYKTQL
STQTYSLNEV AKEILGEEKI YDYENMLYDW AIGNYNKVFE YNLKDAELTY KLFKYYENDL
LELARLVNQP LFDVSRFSYS NIVEWYLIKK SRKYNEIVPN KPKMEEVERR KLNTYAGAFV
YEPKPGLYEN LAVLDFASLY PSIILEHNVS PGTIYCEHDD CKQNGVEAII NNEKKYVWFC
KKVKGFIPTV LEHLYTKRLE LKRKLKELDR DSEEYKIINA KQAVLKIIIN ATYGYMGFPN
ARWYCIDCAA AVAAWGRKYI NYILKRAEEE GFKVIYGDSI MDTEIEVIEN GIKKKEKLSD
LFNKYYAGFQ IGEKHYAFPP DLYVYDGERW VKVYSIIKHE TETDLYEING ITLSANHLVL
SKGNWVKAKE YENKNN
//
