ID NCSA_DICDI Reviewed; 186 AA.
AC Q75K28; Q552Q1;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 27-MAR-2024, entry version 101.
DE RecName: Full=Calcium-binding protein NCSA;
DE AltName: Full=Neuronal calcium sensor 1 homolog;
GN Name=ncsA; ORFNames=DDB_G0275931;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CALCIUM-BINDING, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=AX4;
RX PubMed=15606490; DOI=10.1111/j.1440-169x.2004.00761.x;
RA Coukell B., Cameron A., Perusini S., Shim K.;
RT "Disruption of the NCS-1/frequenin-related ncsA gene in Dictyostelium
RT discoideum accelerates development.";
RL Dev. Growth Differ. 46:449-458(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: May prevent cells from entering development prematurely in
CC the presence of environmental nutrients. {ECO:0000269|PubMed:15606490}.
CC -!- DEVELOPMENTAL STAGE: Expression peaks during late aggregation and slug
CC stage. {ECO:0000269|PubMed:15606490}.
CC -!- SIMILARITY: Belongs to the recoverin family. {ECO:0000305}.
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DR EMBL; AY655128; AAT72744.1; -; mRNA.
DR EMBL; AAFI02000013; EAL69716.1; -; Genomic_DNA.
DR RefSeq; XP_643630.1; XM_638538.1.
DR AlphaFoldDB; Q75K28; -.
DR SMR; Q75K28; -.
DR STRING; 44689.Q75K28; -.
DR PaxDb; 44689-DDB0231007; -.
DR EnsemblProtists; EAL69716; EAL69716; DDB_G0275931.
DR GeneID; 8620217; -.
DR KEGG; ddi:DDB_G0275931; -.
DR dictyBase; DDB_G0275931; ncsA.
DR eggNOG; KOG0044; Eukaryota.
DR HOGENOM; CLU_072366_1_0_1; -.
DR InParanoid; Q75K28; -.
DR OMA; EYVFNVF; -.
DR PhylomeDB; Q75K28; -.
DR Reactome; R-DDI-2514859; Inactivation, recovery and regulation of the phototransduction cascade.
DR PRO; PR:Q75K28; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005509; F:calcium ion binding; IDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR CDD; cd00051; EFh; 3.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR028846; Recoverin.
DR PANTHER; PTHR23055; CALCIUM BINDING PROTEINS; 1.
DR PANTHER; PTHR23055:SF195; NEURONAL CALCIUM SENSOR 1; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR PRINTS; PR00450; RECOVERIN.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW Calcium; Developmental protein; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..186
FT /note="Calcium-binding protein NCSA"
FT /id="PRO_0000323768"
FT DOMAIN 40..58
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 66..93
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 94..129
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 142..177
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 107
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 109
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 111
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 113
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 118
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 155
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 157
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 159
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 161
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 166
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 64
FT /note="D -> N (in Ref. 1; AAT72744)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 186 AA; 21336 MW; 6923840C0C3D0E2C CRC64;
MGNQLGTLKK EEVELLQNSS HFDSRELRAL YKQFRKDSPS GTINKQEFKE IMTQMGVGDQ
FLQDLLFNVF DKNKDSTINF QEFVCGLSSI TRGTPEEKIE FAFSLYDIDG NGYITRSEME
SILESMYKLV GTFVTCSGKK FDPHDLIEEF FDSMDDDGDG YISLEEYKRG TLKNPDIIQG
LKLLNQ
//
