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Database: UniProt
Entry: Q7DI68
LinkDB: Q7DI68
Original site: Q7DI68 
ID   Q7DI68_ECO57            Unreviewed;       319 AA.
AC   Q7DI68; A0A2P0HM18; A0A4Q2TPI0; Q7DBQ5;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 1.
DT   10-FEB-2021, entry version 133.
DE   RecName: Full=rRNA N-glycosylase {ECO:0000256|ARBA:ARBA00012001};
DE            EC=3.2.2.22 {ECO:0000256|ARBA:ARBA00012001};
GN   Name=stx2 A-subunit {ECO:0000313|EMBL:BAB33306.1};
GN   Synonyms=stx 2 A-subunit {ECO:0000313|EMBL:BAB33308.1}, stx2A
GN   {ECO:0000313|EMBL:BAB34628.1};
GN   ORFNames=ECs_1205 {ECO:0000313|EMBL:BAB34628.1};
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334 {ECO:0000313|EMBL:BAB33306.1};
RN   [1] {ECO:0000313|EMBL:BAB34628.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Sakai {ECO:0000313|EMBL:BAB34628.1};
RX   PubMed=10734605; DOI=10.1266/ggs.74.227;
RA   Makino K., Yokoyama K., Kubota Y., Yutsudo C.H., Kimura S., Kurokawa K.,
RA   Ishii K., Hattori M., Tatsuno I., Abe H., Iida T., Yamamoto K., Onishi M.,
RA   Hayashi T., Yasunaga T., Honda T., Sasakawa C., Shinagawa H.;
RT   "Complete nucleotide sequence of the prophage VT2-Sakai carrying the
RT   verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived
RT   from the Sakai outbreak.";
RL   Genes Genet. Syst. 74:227-239(1999).
RN   [2] {ECO:0000313|EMBL:BAB34628.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Sakai {ECO:0000313|EMBL:BAB34628.1};
RX   PubMed=11111050; DOI=10.1016/S0378-1119(00)00416-9;
RA   Yokoyama K., Makino K., Kubota Y., Watanabe M., Kimura S., Yutsudo C.H.,
RA   Kurokawa K., Ishii K., Hattori M., Tatsuno I., Abe H., Yoh M., Iida T.,
RA   Ohnishi M., Hayashi T., Yasunaga T., Honda T., Sasakawa C., Shinagawa H.;
RT   "Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga
RT   toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain
RT   derived from the Sakai outbreak.";
RL   Gene 258:127-139(2000).
RN   [3] {ECO:0000313|EMBL:BAB34628.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Sakai {ECO:0000313|EMBL:BAB34628.1};
RX   PubMed=11108008;
RA   Ohnishi M., Murata T., Nakayama K., Kuhara S., Hattori M., Kurokawa K.,
RA   Yasunaga T., Yokoyama K., Makino K., Shinagawa H., Hayashi T.;
RT   "Comparative analysis of the whole set of rRNA operons between an
RT   enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia
RT   coli K-12 strain MG1655.";
RL   Syst. Appl. Microbiol. 23:315-324(2000).
RN   [4] {ECO:0000313|EMBL:BAB34628.1, ECO:0000313|Proteomes:UP000000558}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 /
RC   EHEC {ECO:0000313|Proteomes:UP000000558}, and Sakai
RC   {ECO:0000313|EMBL:BAB34628.1};
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [5] {ECO:0000313|EMBL:BAB33306.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=#A-1 {ECO:0000313|EMBL:BAB33306.1}, and #A-2
RC   {ECO:0000313|EMBL:BAB33308.1};
RX   PubMed=11561972; DOI=10.1017/S0950268801005635;
RA   Asakura H., Makino S., Kobori H., Watarai M., Shirahata T., Ikeda T.,
RA   Takeshi K.;
RT   "Phylogenetic diversity and similarity of active sites of Shiga toxin (stx)
RT   in Shiga toxin-producing Escherichia coli (STEC) isolates from humans and
RT   animals.";
RL   Epidemiol. Infect. 127:27-36(2001).
RN   [6] {ECO:0000213|PDB:4M1U}
RP   X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) OF 23-319, AND DISULFIDE BONDS.
RX   PubMed=24225957; DOI=10.1074/jbc.m113.518886;
RA   Jacobson J.M., Yin J., Kitov P.I., Mulvey G., Griener T.P., James M.N.,
RA   Armstrong G., Bundle D.R.;
RT   "The crystal structure of shiga toxin type 2 with bound disaccharide guides
RT   the design of a heterobifunctional toxin inhibitor.";
RL   J. Biol. Chem. 289:885-894(2014).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000256|ARBA:ARBA00000237};
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DR   EMBL; AB048836; BAB33306.1; -; Genomic_DNA.
DR   EMBL; AB048837; BAB33308.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34628.1; -; Genomic_DNA.
DR   RefSeq; NP_309232.1; NC_002695.1.
DR   RefSeq; WP_000649753.1; NZ_SEKU01000005.1.
DR   PDB; 4M1U; X-ray; 1.56 A; A=23-319.
DR   PDBsum; 4M1U; -.
DR   STRING; 155864.EDL933_1383; -.
DR   EnsemblBacteria; AAG55587; AAG55587; Z1464.
DR   EnsemblBacteria; BAB34628; BAB34628; BAB34628.
DR   GeneID; 912807; -.
DR   KEGG; ecs:ECs1205; -.
DR   PATRIC; fig|386585.9.peg.1307; -.
DR   HOGENOM; CLU_870802_0_0_6; -.
DR   Proteomes; UP000000558; Chromosome.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   Gene3D; 4.10.470.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   InterPro; IPR016331; Shiga-like_toxin_subunit_A.
DR   PANTHER; PTHR33453; PTHR33453; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PIRSF; PIRSF001924; Shigella_toxin_subunit_A; 1.
DR   SUPFAM; SSF56371; SSF56371; 1.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0000213|PDB:4M1U};
KW   Disulfide bond {ECO:0000256|PIRSR:PIRSR001924-3};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Protein synthesis inhibitor {ECO:0000256|ARBA:ARBA00023193};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000558};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW   Toxin {ECO:0000256|ARBA:ARBA00022656}.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           23..319
FT                   /note="rRNA N-glycosylase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5014310892"
FT   ACT_SITE        189
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001924-1"
FT   DISULFID        263..282
FT                   /evidence="ECO:0000213|PDB:4M1U,
FT                   ECO:0000256|PIRSR:PIRSR001924-3"
SQ   SEQUENCE   319 AA;  35714 MW;  98F73319ACAE48D6 CRC64;
     MKCILFKWVL CLLLGFSSVS YSREFTIDFS TQQSYVSSLN SIRTEISTPL EHISQGTTSV
     SVINHTPPGS YFAVDIRGLD VYQARFDHLR LIIEQNNLYV AGFVNTATNT FYRFSDFTHI
     SVPGVTTVSM TTDSSYTTLQ RVAALERSGM QISRHSLVSS YLALMEFSGN TMTRDASRAV
     LRFVTVTAEA LRFRQIQREF RQALSETAPV YTMTPGDVDL TLNWGRISNV LPEYRGEDGV
     RVGRISFNNI SAILGTVAVI LNCHHQGARS VRAVNEESQP ECQITGDRPV IKINNTLWES
     NTAAAFLNRK SQFLYTTGK
//
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