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Database: UniProt
Entry: Q7N8V1
LinkDB: Q7N8V1
Original site: Q7N8V1 
ID   RAPA_PHOLL              Reviewed;         970 AA.
AC   Q7N8V1;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   16-OCT-2019, entry version 102.
DE   RecName: Full=RNA polymerase-associated protein RapA {ECO:0000255|HAMAP-Rule:MF_01821};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_01821};
DE   AltName: Full=ATP-dependent helicase HepA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   Name=rapA {ECO:0000255|HAMAP-Rule:MF_01821}; Synonyms=hepA;
GN   OrderedLocusNames=plu0615;
OS   Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565
OS   / TT01).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=243265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15139 / CIP 105565 / TT01;
RX   PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S.,
RA   Medigue C., Lanois A., Powell K., Siguier P., Vincent R., Wingate V.,
RA   Zouine M., Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- FUNCTION: Transcription regulator that activates transcription by
CC       stimulating RNA polymerase (RNAP) recycling in case of stress
CC       conditions such as supercoiled DNA or high salt concentrations.
CC       Probably acts by releasing the RNAP, when it is trapped or
CC       immobilized on tightly supercoiled DNA. Does not activate
CC       transcription on linear DNA. Probably not involved in DNA repair.
CC       {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SUBUNIT: Interacts with the RNAP. Has a higher affinity for the
CC       core RNAP than for the holoenzyme. Its ATPase activity is
CC       stimulated by binding to RNAP. {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. RapA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01821}.
DR   EMBL; BX571861; CAE12910.1; -; Genomic_DNA.
DR   RefSeq; WP_011144992.1; NC_005126.1.
DR   STRING; 243265.plu0615; -.
DR   PRIDE; Q7N8V1; -.
DR   EnsemblBacteria; CAE12910; CAE12910; plu0615.
DR   KEGG; plu:plu0615; -.
DR   eggNOG; ENOG4105BZK; Bacteria.
DR   eggNOG; COG0553; LUCA.
DR   HOGENOM; HOG000218482; -.
DR   KO; K03580; -.
DR   OMA; MSILERD; -.
DR   OrthoDB; 291634at2; -.
DR   BioCyc; PLUM243265:PLU_RS03030-MONOMER; -.
DR   Proteomes; UP000002514; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   HAMAP; MF_01821; Helicase_RapA; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR023949; Helicase_RapA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022737; RapA_C.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   InterPro; IPR040765; Tudor_1_RapA.
DR   InterPro; IPR040766; Tudor_2_RapA.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF12137; RapA_C; 1.
DR   Pfam; PF00176; SNF2_N; 1.
DR   Pfam; PF18339; Tudor_1_RapA; 1.
DR   Pfam; PF18337; Tudor_RapA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; Complete proteome; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    970       RNA polymerase-associated protein RapA.
FT                                /FTId=PRO_0000207179.
FT   DOMAIN      164    334       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   DOMAIN      490    663       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   NP_BIND     177    184       ATP. {ECO:0000255|HAMAP-Rule:MF_01821}.
FT   MOTIF       280    283       DEAH box.
SQ   SEQUENCE   970 AA;  111060 MW;  9D9FF7622B67FB71 CRC64;
     MPFTLGQRWI SDTESELGLG TVVALDARMV TLLFPASGEN RLYARNDSPI TRVMFNSGDV
     VTSHEGWQLK VDEIQQENGL LIYTGTRLDT LEENISLREV FLDSKLTFNK PQDRLFAGQI
     DRMDRFALRF RARKYQSEQF KLAESGLRGI RASLIPHQLH IANEVGKRYA PRVLLADEVG
     LGKTIEAGMI IHQQLLAGRA ERVLVIVPDS LQHQWLVEML RRFNLRFALF DDSRYIEARH
     DSDNPFETEQ LVLCSLDFAR RNKQRFEHML EASWDLMVVD EAHHLVWSED APSREYQVVE
     QLAEQIPSVL LLTATPEQLG QESHFARLRL LDPNRFHDYQ TFIDEQQKYR PVADAVTLLL
     SGEQLNNNQL NLMGELISEQ NIEPLLKAAN SNSGESETAR RELISMLMDR HGTSRILFRN
     TRNGVKGFPH RELHQIKLPL PSQYQTAIKV SDIMGAKKSL EARAWDMLYP EQIYQEFEGE
     NATWWNFDPR VEWLLGFLMA NRHEKVLVIC AKATTALQLE QVLREREGIR GAVFHEGLSL
     IERDRAAAYF ASEEEGAQVL LCSEIGSEGR NFQFANQLVM FDLPFNPDLL EQRIGRLDRI
     GQSRDIQISV PYLENTAQAV LIRWYHEGLD AFEHTCPTGR TIYDHYYEAL LNYLAKPNNQ
     DQEKFGEFID QCRQQHEQLK QQLEQGRDRL LEMNSNGGEH GLRLAEKISE YDNDTELVNF
     SLNLFDIVGI NQEDRNDNMI VLTPSDHMLV PDFPGLPKDG CTITFDREQS LSREDAQFIS
     WEHPIIRNGL DLILSGDTGS CAVSLLKNKA LPVGTLLVEL IYVVEAQAPK RLQLTRFLPP
     TPLRILMDLK GNNLADQVEF ESFNRQLNAV NRHTASKLVN AVQKEVHAIL QQAEGLVETQ
     AKSLIEQAEQ EADENLAAEL SRLEALKTVN PNIRDDELEA IEANRQQLLL NLNQASWRLD
     AIRLVVVTHQ
//
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