ID Q7RNG8_PLAYO Unreviewed; 1294 AA.
AC Q7RNG8;
DT 15-DEC-2003, integrated into UniProtKB/TrEMBL.
DT 15-DEC-2003, sequence version 1.
DT 24-JAN-2024, entry version 123.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=PY01849 {ECO:0000313|EMBL:EAA21241.1};
OS Plasmodium yoelii yoelii.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Vinckeia).
OX NCBI_TaxID=73239 {ECO:0000313|EMBL:EAA21241.1, ECO:0000313|Proteomes:UP000008553};
RN [1] {ECO:0000313|EMBL:EAA21241.1, ECO:0000313|Proteomes:UP000008553}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17XNL {ECO:0000313|EMBL:EAA21241.1,
RC ECO:0000313|Proteomes:UP000008553};
RX PubMed=12368865; DOI=10.1038/nature01099;
RA Carlton J.M., Angiuoli S.V., Suh B.B., Kooij T.W., Pertea M., Silva J.C.,
RA Ermolaeva M.D., Allen J.E., Selengut J.D., Koo H.L., Peterson J.D., Pop M.,
RA Kosack D.S., Shumway M.F., Bidwell S.L., Shallom S.J., van Aken S.E.,
RA Riedmuller S.B., Feldblyum T.V., Cho J.K., Quackenbush J., Sedegah M.,
RA Shoaibi A., Cummings L.M., Florens L., Yates J.R., Raine J.D., Sinden R.E.,
RA Harris M.A., Cunningham D.A., Preiser P.R., Bergman L.W., Vaidya A.B.,
RA van Lin L.H., Janse C.J., Waters A.P., Smith H.O., White O.R.,
RA Salzberg S.L., Venter J.C., Fraser C.M., Hoffman S.L., Gardner M.J.,
RA Carucci D.J.;
RT "Genome sequence and comparative analysis of the model rodent malaria
RT parasite Plasmodium yoelii yoelii.";
RL Nature 419:512-519(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAA21241.1}.
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DR EMBL; AABL01000503; EAA21241.1; -; Genomic_DNA.
DR STRING; 73239.Q7RNG8; -.
DR PaxDb; 73239-Q7RNG8; -.
DR EnsemblProtists; EAA21241; EAA21241; EAA21241.
DR VEuPathDB; PlasmoDB:Py17XNL_001303372; -.
DR InParanoid; Q7RNG8; -.
DR OMA; EIIVIHK; -.
DR Proteomes; UP000008553; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000008553}.
FT DOMAIN 102..828
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 884..1073
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT REGION 992..1021
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1294 AA; 153351 MW; 9E4120FD969CDC08 CRC64;
MFLKRIKQKV QFIRKFKTLK SEIASVKIEI KKKENNICTV QNIFCEKKKK KIHTIIEKIG
HEYPRNTHKI NSPKEKKNSE MATKTTFDYV PDNPNIVEEE EKILKYWKNI NAFELSNKLS
KNRKPYIFYD GPPFATGLPH YGHLLAGIIK DCVTRYFYQS GYSVERRFGW DCHGLPVEYE
IEKENNINKK EDILKMGIDV YNEKCRSIVL KYSNEWVNTV ERIGRWIDYK NDYKTMDKDF
MESVWWIFSQ LYKKNYIYKS FKVMPYSCKC NTPISNFELN LNYKDVSDPS IIVGFILLEN
FPTFPECLEI QKEIDASDSP TKILNEKPQD GKKEEIDFFK KYEKLYEPYF SRVKSNELKT
SNFDENKKRE DFSESTSDTS NKVTEIIAWT TTPWTLPSNL ALCVNENLIY LRILNKENNR
IFIFAECRME WVIKELRLKI ENICLLNRFK GKYLNGLKYK PLFNHFYEKF NFKENAYRIL
CDEFVTNTVG TGIVHCAPTY GEDDFRVCEK NKIINPEKSI FIDPLDSNGY FTNEIKEVEN
MYIKDADIVI KKILKEQNRL LSNNMIVHSY PFCWRSDTPL IYRAIPAWFV RVNNYTDKLV
KNNDTTYWIP AHVKEKKFHN WIKDAKDWCI SRNRYWGTPI PIWTDEKMEQ IICIESIKQL
ETLSGVTNIS DLHRHNIDHI TIDNPRGKNY PKLKRIPEVF DCWFESGSMP YAKVHFPFYC
SDIANSEIDI SKVSMDQFNQ IFPADFIAEG LDQTRGWFYT LLVISTLLFD KAPYKNLICN
GLILASDGKK MSKRLKNYPD PIYILNKYGA DSLRLYLINS VAVRAENLKF QEKGVNEIVK
SFILPFYHSF RFFSQEVIRY ETSYKTTFSF SNKYIYDNNN NIMDQWIFSS IQNLIKSVHY
EMKQYKLYNV LPKLLNFIEN LTNWYIRLNR DRMRGVIDEI NCLMSLNTIY KTLYLFTLMM
SPFTPFISEY IYKMLQQVQG FNLVMSSDTH KNVQNGQNVQ NDQNGQNVQN GQNDQNDQND
QNKSVHFHML PEVDDNYTIR YEIIELIEKM KSVIILGRIL REKRKCPNKR PLKSIKILHR
ENKFFENFDK IANYIKDELN ILNIEYSTDV SCLTFSVIPN YKKLGTKLGA NLKNIQNKIK
NMNMEDIKNY EQNKKIIIDN ILLEDDDIII NLKHNLNLEH IEAISENSIT ILMDFTADEQ
LNNMENAREI CNNIQKMRKK LALSQNSPVQ MHIFIQNETF KNNMIKETEY IHKCLRRNLN
IINTVDQMEK LQNKFHQETF TVSGQEVIAI FTQN
//
