GenomeNet

Database: UniProt
Entry: Q7SEL0
LinkDB: Q7SEL0
Original site: Q7SEL0 
ID   PRP28_NEUCR             Reviewed;         728 AA.
AC   Q7SEL0;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   31-JUL-2019, entry version 105.
DE   RecName: Full=Pre-mRNA-splicing ATP-dependent RNA helicase prp-28;
DE            EC=3.6.4.13;
GN   Name=prp-28; ORFNames=NCU02803;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM
OS   1257 / FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae;
OC   Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A.,
RA   Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L.,
RA   Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C.,
RA   Marcotte E., Greenberg D., Roy A., Foley K., Naylor J.,
RA   Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M.,
RA   Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S.,
RA   Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C.,
RA   Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M.,
RA   Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved in mRNA splicing.
CC       May destabilize the U1/5'-splice site duplex to permit an
CC       effective competition for the 5'-splice site by the U6 snRNA,
CC       resulting in the switch between U1 and U6 at the 5'-splice site.
CC       May also act to unwind the U4/U6 base-pairing interaction in the
CC       U4/U6/U5 snRNP, facilitating the first covalent step of splicing
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Component of the U5 snRNP complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus
CC       {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX23/PRP28
CC       subfamily. {ECO:0000305}.
DR   EMBL; CM002236; EAA35223.1; -; Genomic_DNA.
DR   RefSeq; XP_964459.1; XM_959366.2.
DR   SMR; Q7SEL0; -.
DR   PRIDE; Q7SEL0; -.
DR   EnsemblFungi; EAA35223; EAA35223; NCU02803.
DR   GeneID; 3880623; -.
DR   KEGG; ncr:NCU02803; -.
DR   EuPathDB; FungiDB:NCU02803; -.
DR   HOGENOM; HOG000268796; -.
DR   InParanoid; Q7SEL0; -.
DR   KO; K12858; -.
DR   OMA; TEKKFNF; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase;
KW   mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN         1    728       Pre-mRNA-splicing ATP-dependent RNA
FT                                helicase prp-28.
FT                                /FTId=PRO_0000232376.
FT   DOMAIN      324    527       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      538    701       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     337    344       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       293    321       Q motif.
FT   MOTIF       450    453       DEAD box.
SQ   SEQUENCE   728 AA;  82612 MW;  60AFF9AAA19E8B27 CRC64;
     MSTTSRREPP DLAALLRKKK EEEAAAAKPR FIPKKERERL EAEKKAKEEE ERKRKEEAKP
     QPNGTNHNGN RMDGIQSHHN HNPQRNIPTG PKAMRYDDDR GPNGMSNGRD YRDNRDNRDN
     RDRNQRGAKR GAPNDDEEKR AKMERNDEAE LRARYMGPVV NQSTFSAKKK RRRTAANKFN
     FDWDADDDTS RPFDPIYAER QEPLVRLGGY EMTEEMVMRK AEAIRRGDPE TGEERARQYL
     EQHRRIKEME QRKNLGKHWS EKKLEDMKER DWRIFKENFG IATKGGAIPN PMRSWEESTL
     PRRLLDIVKN VGYDEPTPIQ RAAIPIALQA RDLIGVAVTG SGKTAAFLLP LLVYISELPP
     LTEYNKNDGP YALILAPTRE LVQQIESEAK KFATPLGFTV VSIVGGHSLE EQAFALRNGA
     EIIVATPGRL VDCLERRLLV FSQCCYTIMD EADRMIDQGF EEPLTKILDA MPVTNEKPDT
     DDAENPQLMS RYVDGKDRYR QTMMYTATMP PIVERIAKKY LRRPAIVTIG NAGEAVDTVE
     QRVEFVSGED KRKKRLQEIL NSGQFKPPII VFVNIKRNCD MVARDIKGMG YSAVTLHGSK
     TQEQREAALA SLRNGQTDIL VATDLAGRGI DVPDVSLVVN FNMATNIESY THRIGRTGRA
     GKSGVAITFL GPEDNDVLYD LRQIISKSSI SKVPDELRRH EAAQNKPQKG QKKLEESNGY
     SGKGGSWN
//
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