ID Q7VNI3_HAEDU Unreviewed; 1158 AA.
AC Q7VNI3;
DT 01-OCT-2003, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2003, sequence version 1.
DT 27-MAR-2024, entry version 125.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=dnaE {ECO:0000313|EMBL:AAP95487.1};
GN OrderedLocusNames=HD_0551 {ECO:0000313|EMBL:AAP95487.1};
OS Haemophilus ducreyi (strain 35000HP / ATCC 700724).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=233412 {ECO:0000313|EMBL:AAP95487.1, ECO:0000313|Proteomes:UP000001022};
RN [1] {ECO:0000313|Proteomes:UP000001022}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=35000HP / ATCC 700724 {ECO:0000313|Proteomes:UP000001022};
RA Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L.,
RA Nguyen D., Wang J., Forst C., Hood L.;
RT "The complete genome sequence of Haemophilus ducreyi.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; AE017143; AAP95487.1; -; Genomic_DNA.
DR RefSeq; WP_010944540.1; NC_002940.2.
DR AlphaFoldDB; Q7VNI3; -.
DR STRING; 233412.HD_0551; -.
DR KEGG; hdu:HD_0551; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_0_0_6; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000001022; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR048472; DNA_pol_IIIA_C.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF20914; DNA_pol_IIIA_C; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000001022};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1158 AA; 129602 MW; B75D10DDFDA1FED6 CRC64;
MTQPRFVHLK VHSDFSMING LAKVKALVKT AAAKQMAAMA LTDFSNFCGL VKFYGESLTA
GLKPIIGADI YVRSDPTSED IFELTLLAKN NQGYHNITVL LSRAYQQGYV EFPIVEKAWL
AELNEGIIVL SGGRSGDVGK ALLKENEQEA ATLVAFYQQY FADHYYLSLC RTGRSDEERY
LQQAVQFAKK YQLPLVAVND VVFLKEDDFD AHEIRVAIHD SYTLDDPKRP QKYSPQQYFR
SEEEMCALFA DLPQALMNSI QIAMRCNVTI RLGEYFLPNF PTGELSTEDF LIKKSQEGLE
ERLAFLFPDA ETRKNKRSVY DERLQVELDV INQMGFPGYF LIVMEFIQWS KDNDIPVGPG
RGSGAGSLVA YALKITDLDP LQFDLLFERF LNPERISMPD FDVDFCMDGR DHVIEHVADT
YGRQAVSQII TFGTMAAKAV IRDVGRVLGH PYNFVDRISK LIPADPGMTL AKAFEAEPKL
PELYNADEEV KDLIDMARKL EGVTRNAGKH AGGVVIAPTA ITDFSPLYCD SEGLHPVTHF
DKNDVEYAGL VKFDFLGLRT LTIIKWALEM INQRLTREGK PPIRIENIPL DDKPSFKLLL
AAKTTAVFQL ESRGMKDLIS RLKPDCFEDI IALVALFRPG PLESGMVQNF IDRKHGIEEV
SYPDAQYQHE CLKPILEPTY GIIVYQEQVM QIAQELAGYT LGSADLLRRA MGKKKPEEMA
AQRDIFEKGA IEKGIDGQLA IKIFDLVEKF AGYGFNKSHS AAYALVSYQT LWLKAHYPAE
FMAAVMTSEM DNTDKIVGLY DECINMGLTV VPPDVNSGKH RFSVNEKGQI VYGLGAIKGV
GEGPIEAILE ARNKGGIFKD LFDLTARVDL KKINRRTFES LIMSGAFDKL GPHRAALMKN
LEDALKASDQ HSKMEALGQS DMFGVLTETP EQVQKAYANT PKWSEQAVLE GERTTLGLYL
SGHPIGRFLK ELAHYAPTRL NELQPTRRGQ MVTVAGIIVA SRIAVTKRGS RLGIATLEDR
AGKIDITLFS EALENYGDLL VKDNIIIATG SVQSDDFNGG LKMSVREIAN LDEIRSRYAK
SLALAISQQQ LTPQFVKALR EIIEPYKEGT LPLHFYYQSP EGRALLRGGV EWRVTPKDEM
LDKLAVLLGE NAVELEFE
//
