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Database: UniProt
Entry: Q7XD96
LinkDB: Q7XD96
Original site: Q7XD96 
ID   DCL3B_ORYSJ             Reviewed;        1637 AA.
AC   Q7XD96; Q0IWV3; Q8W367;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   16-OCT-2019, entry version 105.
DE   RecName: Full=Endoribonuclease Dicer homolog 3b;
DE   AltName: Full=Dicer-like protein 3b;
DE            Short=OsDCL3b;
DE            EC=3.1.26.-;
GN   Name=DCL3B; OrderedLocusNames=Os10g0485600, LOC_Os10g34430;
GN   ORFNames=OSJNBa0029C15.23;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12791992; DOI=10.1126/science.1083523;
RA   Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K.,
RA   Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S.,
RA   Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H.,
RA   Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D.,
RA   Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L.,
RA   De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S.,
RA   Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B.,
RA   Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A.,
RA   Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z.,
RA   Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S.,
RA   Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T.,
RA   Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G.,
RA   Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T.,
RA   Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H.,
RA   Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N.,
RA   Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA   Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S.,
RA   Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT   "In-depth view of structure, activity, and evolution of rice
RT   chromosome 10.";
RL   Science 300:1566-1569(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18826656; DOI=10.1186/1471-2164-9-451;
RA   Kapoor M., Arora R., Lama T., Nijhawan A., Khurana J.P., Tyagi A.K.,
RA   Kapoor S.;
RT   "Genome-wide identification, organization and phylogenetic analysis of
RT   dicer-like, argonaute and RNA-dependent RNA polymerase gene families
RT   and their expression analysis during reproductive development and
RT   stress in rice.";
RL   BMC Genomics 9:451-451(2008).
CC   -!- FUNCTION: Probably involved in the RNA silencing pathway. May
CC       cleave double-stranded RNA to produce short 21-24 nucleotides (nt)
CC       RNAs which target the selective destruction of complementary RNAs
CC       (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- SUBUNIT: May interact with ARGONAUTE1 or PINHEAD through their
CC       common PAZ domains. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the helicase family. Dicer subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00657}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL59041.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAP54346.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF26812.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AC087182; AAL59041.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DP000086; AAP54346.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008216; BAF26812.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014966; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   STRING; 4530.OS10T0485600-00; -.
DR   PaxDb; Q7XD96; -.
DR   PRIDE; Q7XD96; -.
DR   EnsemblPlants; Os10t0485600-01; Os10t0485600-01; Os10g0485600.
DR   Gramene; Os10t0485600-01; Os10t0485600-01; Os10g0485600.
DR   eggNOG; ENOG410INVH; Eukaryota.
DR   eggNOG; KOG0701; Eukaryota.
DR   eggNOG; COG0571; LUCA.
DR   eggNOG; COG1111; LUCA.
DR   HOGENOM; HOG000030250; -.
DR   InParanoid; Q7XD96; -.
DR   Proteomes; UP000059680; Chromosome 10.
DR   Genevisible; Q7XD96; OS.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   CDD; cd00593; RIBOc; 2.
DR   Gene3D; 1.10.1520.10; -; 1.
DR   Gene3D; 3.30.160.380; -; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR038248; Dicer_dimer_sf.
DR   InterPro; IPR005034; Dicer_dimerisation_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003100; PAZ_dom.
DR   InterPro; IPR036085; PAZ_dom_sf.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF03368; Dicer_dimer; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF02170; PAZ; 1.
DR   Pfam; PF00636; Ribonuclease_3; 2.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00949; PAZ; 1.
DR   SMART; SM00535; RIBOc; 2.
DR   SUPFAM; SSF101690; SSF101690; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF69065; SSF69065; 2.
DR   PROSITE; PS51327; DICER_DSRBF; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50821; PAZ; 1.
DR   PROSITE; PS00517; RNASE_3_1; 1.
DR   PROSITE; PS50142; RNASE_3_2; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Endonuclease; Helicase; Hydrolase;
KW   Magnesium; Manganese; Metal-binding; Nuclease; Nucleotide-binding;
KW   Nucleus; Reference proteome; Repeat; RNA-binding;
KW   RNA-mediated gene silencing.
FT   CHAIN         1   1637       Endoribonuclease Dicer homolog 3b.
FT                                /FTId=PRO_0000378420.
FT   DOMAIN       46    222       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      404    556       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   DOMAIN      581    671       Dicer dsRNA-binding fold.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00657}.
FT   DOMAIN      867   1006       PAZ. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00142}.
FT   DOMAIN     1031   1200       RNase III 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00177}.
FT   DOMAIN     1241   1389       RNase III 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00177}.
FT   DOMAIN     1412   1481       DRBM 1.
FT   DOMAIN     1545   1629       DRBM 2.
FT   NP_BIND      59     66       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       169    172       DECH box.
FT   METAL      1214   1214       Magnesium or manganese. {ECO:0000250}.
FT   METAL      1309   1309       Magnesium or manganese. {ECO:0000250}.
FT   METAL      1312   1312       Magnesium or manganese. {ECO:0000250}.
FT   SITE       1305   1305       Important for activity. {ECO:0000250}.
SQ   SEQUENCE   1637 AA;  184725 MW;  811F2AB02659B318 CRC64;
     MADDEAAVLP PPPPLPPPCR PHRQLRPRGS RPTADTTPRT SQLVEVFEAA LRGNTIAVLD
     TGSGKTMVAV MLAREHARRV RAGEAPRRIV VFLAPTVHLV HQQFEVIREY TDLDVMMCSG
     ASRVGEWGAD HWKEEVGRNE IVVMTPQILL DALRHAFLTM SAVSLLIFDE CHRACGSHPY
     ARIMKEFYFG SQWRPDVFGM TASPVATKGA STLHNCEAHI SQLELTLDAK IYIVEDRNEL
     ESFSPPTTIV NKYYDAYMVD FDNLKSKLQI FSDEFDSLLV GLQESPSNKF KDTDNILETS
     RKSLSRYHGK ILYSLNDLGP IITSEVVKIH IESVKPLCDS EDCIFSKASL CLHMSYFKEA
     LSLIEEILPQ GYGELMKSES GSEELTKRGY ISSKVNTLIN IFKSFGSSNE VLCLIFVDRI
     ITAKAVERFM RGIVNFSCFS ISYLTGGSTS KDALSPAVQR FTLDLFRAGK VNLLFTTDVT
     EEGVDVPNCS CVIRFDLPRT VCSYVQSRGR ARRNNSEFIL MIERGNLQQQ EHIFRMIQTG
     YYVKNCALYR HPNALSYDLS IQGMYTYQVQ STGATITADC CVNLIRKYCE KLPKDRYFMP
     KPSFEVTIED GLFKCTLTLP RNAAFQSIVG PLSSSSNLSK QLVSLEACKK LHQLGELNDH
     LVPLTEEPMD TDFTTADEKC ISGPGTTKRK ELHGTTCVLA LSGTWIHDSE NITLNTYRID
     FLCDQEGENY AGFVLLMEPE LDDDVAPSKM DLFLIPNKMV YTTVTPRGKV QLNKKQLGKG
     KLFQEFFFNG IFGRLFHGSR KSGAQRDFIF KKGHEIQWNT ESMYLLLPLR DSSYIQDDLS
     IHWEAIESCA GAVEQLWSSY QGDENVIPVN CIPQKRRGGQ EEIIHLANKS LHCSSIKDSV
     VLSLHTGRIY TVLDLILDTT AEDSFDEMCK GKASPFTSFV DYYHQKYGII IQHPEQPLLL
     LKQSHNAHNL LFSKLKYLDG STGKPLLMEK EQIHARVPPE LLIHLDVTTD ILKSFYLLPS
     VIHRLQSLML ASQLRREIGY NQHIPVTLIL EAITTLRCCE TFSLERLELL GDSVLKYVVG
     CDLFLRYPMK HEGQLSDMRS KAVCNATLHK HGIWRSLQGY VRDNAFDPRR WVAPGQISLR
     PFPCNCGIET AFVPSHRRYI RDDPSFFVGK PCDRGHRWMC SKTISDCVEA LVGAYYVGGG
     IAAALWVMRW FGIDIKCDMK LLQEVKFNAS HLCSLSKIND IEELEAKLKY NFSVKGLLLE
     AITHPSLQEL GVDYCYQRLE FLGDSVLDLL LTRHLYATHT DVDPGELTDL RSALVSNENF
     AQAVVRNNIH SHLQHGSGIL LEQITEYVRS NLECQGKESE FLQHTTCKVP KVLGDIMESI
     AGAVFIDTDF NVDMVWEIFE PLLSPLITPD KLALPPYREL LELCSHIGCF LNSKCTSKGE
     EVIIEMSLQL RDELLVAQGH DRNKKRAKAK AASRILADLK QQGLSIKQCL SKAKQLDIVT
     SDLQFDLTSS GTQLSYSDLN DYHILEGLSS VKKEVVLPLK MEKGGPRSAL FKLCKILQWP
     MPEFEFVEQR FRTPIVMDGA TTTNFNSFVS TITLHIPDAT TITFQGERRT DKKSAQDSAS
     LMMLHKLQEL KICICKT
//
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