ID Q82SP5_NITEU Unreviewed; 392 AA.
AC Q82SP5;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE SubName: Full=Thiolase {ECO:0000313|EMBL:CAD86174.1};
DE EC=2.3.1.9 {ECO:0000313|EMBL:CAD86174.1};
GN Name=bktB {ECO:0000313|EMBL:CAD86174.1};
GN OrderedLocusNames=NE2262 {ECO:0000313|EMBL:CAD86174.1};
OS Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS 14298).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosomonas.
OX NCBI_TaxID=228410 {ECO:0000313|EMBL:CAD86174.1, ECO:0000313|Proteomes:UP000001416};
RN [1] {ECO:0000313|EMBL:CAD86174.1, ECO:0000313|Proteomes:UP000001416}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298
RC {ECO:0000313|Proteomes:UP000001416};
RX PubMed=12700255; DOI=10.1128/JB.185.9.2759-2773.2003;
RA Chain P., Lamerdin J., Larimer F., Regala W., Land M., Hauser L.,
RA Hooper A., Klotz M., Norton J., Sayavedra-Soto L., Arciero D., Hommes N.,
RA Whittaker M., Arp D.;
RT "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT chemolithoautotroph Nitrosomonas europaea.";
RL J. Bacteriol. 185:2759-2773(2003).
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000256|ARBA:ARBA00010982, ECO:0000256|RuleBase:RU003557}.
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DR EMBL; AL954747; CAD86174.1; -; Genomic_DNA.
DR RefSeq; WP_011112753.1; NC_004757.1.
DR AlphaFoldDB; Q82SP5; -.
DR STRING; 228410.NE2262; -.
DR KEGG; neu:NE2262; -.
DR eggNOG; COG0183; Bacteria.
DR HOGENOM; CLU_031026_0_0_4; -.
DR OrthoDB; 6139495at2; -.
DR PhylomeDB; Q82SP5; -.
DR Proteomes; UP000001416; Chromosome.
DR GO; GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IEA:UniProtKB-EC.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR InterPro; IPR020610; Thiolase_AS.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR PANTHER; PTHR18919; ACETYL-COA C-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR18919:SF153; TRIFUNCTIONAL ENZYME SUBUNIT BETA, MITOCHONDRIAL; 1.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00098; THIOLASE_1; 1.
DR PROSITE; PS00737; THIOLASE_2; 1.
DR PROSITE; PS00099; THIOLASE_3; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003557,
KW ECO:0000313|EMBL:CAD86174.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001416};
KW Transferase {ECO:0000256|RuleBase:RU003557, ECO:0000313|EMBL:CAD86174.1}.
FT DOMAIN 4..261
FT /note="Thiolase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00108"
FT DOMAIN 270..391
FT /note="Thiolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02803"
FT ACT_SITE 89
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 348
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 378
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
SQ SEQUENCE 392 AA; 41601 MW; F203D007E21E2AD5 CRC64;
MRNVVVLSGA RTAIGDYGGA LKDLAPATLA AITVREAITR ASVDSDKIGH LVAGHVIPTE
AQDMYLARVA ALEAGLPQSV AALTLNRVCG SGLQAIISAA QAVMLGDTEV AIAAGAESMS
RGGYLLPALR WGQRMQDSTA VDMMVGALTD PFDRVHMGIT AENIAERWSI TREQQDRFAL
ESHRRAIHAV HNGYFREQIV PVEVSTREGV AMFDTDQHPR DNISMETLGK LRPVFREDGT
VTAGNASGIN DGAAALVLME ERAAERNHHQ PMARLVAYGH AGVNPRYMGI GPVPAVKQAL
NRAGLDLAEM DVIESNEAFA VQACAVAREL NFDPDKVNPN GGAVALGHPI GASGCILAIK
AIYELQRING RYALVTLCIG GGQGIAAIFE KL
//
