ID Q82YW3_ENTFA Unreviewed; 470 AA.
AC Q82YW3;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 97.
DE RecName: Full=Citrate lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE Short=Citrase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE EC=2.8.3.10 {ECO:0000256|PIRNR:PIRNR009451};
DE EC=4.1.3.6 {ECO:0000256|PIRNR:PIRNR009451};
DE AltName: Full=Citrate (pro-3S)-lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE AltName: Full=Citrate CoA-transferase subunit {ECO:0000256|PIRNR:PIRNR009451};
GN Name=citF {ECO:0000313|EMBL:AAO82984.1};
GN OrderedLocusNames=EF_3319 {ECO:0000313|EMBL:AAO82984.1};
OS Enterococcus faecalis (strain ATCC 700802 / V583).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=226185 {ECO:0000313|EMBL:AAO82984.1, ECO:0000313|Proteomes:UP000001415};
RN [1] {ECO:0000313|EMBL:AAO82984.1, ECO:0000313|Proteomes:UP000001415}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700802 / V583 {ECO:0000313|Proteomes:UP000001415};
RX PubMed=12663927; DOI=10.1126/science.1080613;
RA Paulsen I., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D.,
RA Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA Dodson R.J., Umayam L., Brinkac L., Beanan M., Daugherty S., DeBoy R.T.,
RA Durkin S., Kolonay J., Madupu R., Nelson W., Vamathevan J., Tran B.,
RA Upton J., Hansen T., Shetty J., Khouri H., Utterback T., Radune D.,
RA Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT faecalis.";
RL Science 299:2071-2074(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + citrate = (3S)-citryl-CoA + acetate;
CC Xref=Rhea:RHEA:19405, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57321; EC=2.8.3.10;
CC Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = acetate + oxaloacetate; Xref=Rhea:RHEA:10760,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089; EC=4.1.3.6;
CC Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR009451}.
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DR EMBL; AE016830; AAO82984.1; -; Genomic_DNA.
DR RefSeq; NP_816914.1; NC_004668.1.
DR AlphaFoldDB; Q82YW3; -.
DR STRING; 226185.EF_3319; -.
DR EnsemblBacteria; AAO82984; AAO82984; EF_3319.
DR KEGG; efa:EF3319; -.
DR PATRIC; fig|226185.9.peg.3091; -.
DR eggNOG; COG3051; Bacteria.
DR HOGENOM; CLU_046521_2_0_9; -.
DR Proteomes; UP000001415; Chromosome.
DR GO; GO:0009346; C:ATP-independent citrate lyase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008815; F:citrate (pro-3S)-lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008814; F:citrate CoA-transferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1080.10; Glutaconate Coenzyme A-transferase; 2.
DR InterPro; IPR006472; Citrate_lyase_asu.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR NCBIfam; TIGR01584; citF; 1.
DR PANTHER; PTHR40596; CITRATE LYASE ALPHA CHAIN; 1.
DR PANTHER; PTHR40596:SF1; CITRATE LYASE ALPHA CHAIN; 1.
DR Pfam; PF04223; CitF; 1.
DR PIRSF; PIRSF009451; Citrt_lyas_alpha; 1.
DR SUPFAM; SSF100950; NagB/RpiA/CoA transferase-like; 2.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR009451};
KW Lyase {ECO:0000256|PIRNR:PIRNR009451, ECO:0000313|EMBL:AAO82984.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001415};
KW Transferase {ECO:0000256|PIRNR:PIRNR009451}.
SQ SEQUENCE 470 AA; 50138 MW; 80B3116774432C93 CRC64;
MKPRETKLLG SIREAIEKTG LKDGMTISFH HHFREGDYVM NLVLAEIAAM GLKNISIAPS
SIANVHEPLI EHIKNGVVTN ITSSGLRDKV GAAISAGIME NPVVIRSHGG RARAVAAGDI
HIDVAFLGAP SSDAYGNANG TKGKATCGSL GYAMVDAKYA DQVVIITDTL VPYPNTPISI
PQTDVDYVVE IDAIGDPDGI AKGATRFTKN PKELLIAEYA AKIITHSPYY KEGFSFQTGT
GGSSLAVTRF MREQMLKDGI KASFALGGIT NAMVELLEEG LVEKIIDVQD FDHPSAISLG
ENANHYEIDA NMYASPLSKG AVINQLDTAI LSALEVDTDF NVNVITGSDG VIRGASGGHS
DTSMACKMSL VIAPLVRGRI PTIVEQVNTV VTPGTSVDVV VTEVGIAINP KRTDLIDCFK
TLDVPQFTLE ELKDKAYNIV GTPEPIKYGD KVVALIEYRD GSLIDVVRNV
//
