GenomeNet

Database: UniProt
Entry: Q84WW6
LinkDB: Q84WW6
Original site: Q84WW6 
ID   ASHH1_ARATH             Reviewed;         492 AA.
AC   Q84WW6; C0SV35; Q945S9; Q9SRE2;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   10-APR-2019, entry version 108.
DE   RecName: Full=Histone-lysine N-methyltransferase ASHH1 {ECO:0000305};
DE            EC=2.1.1.43 {ECO:0000305};
DE   AltName: Full=ASH1 homolog 1;
DE   AltName: Full=Protein SET DOMAIN GROUP 26;
GN   Name=ASHH1; Synonyms=SDG26, SET26; OrderedLocusNames=At1g76710;
GN   ORFNames=F28O16.8;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana
RT   reference genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N.,
RA   Takiguchi Y., Takagi M.;
RT   "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 83-311, AND NOMENCLATURE.
RX   PubMed=11691919; DOI=10.1093/nar/29.21.4319;
RA   Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K.,
RA   Assalkhou R., Schulz I., Reuter G., Aalen R.B.;
RT   "The Arabidopsis thaliana genome contains at least 29 active genes
RT   encoding SET domain proteins that can be assigned to four
RT   evolutionarily conserved classes.";
RL   Nucleic Acids Res. 29:4319-4333(2001).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=cv. Columbia;
RX   PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA   Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A.,
RA   Andreasson E., Rathjen J.P., Peck S.C.;
RT   "Phosphoproteomic analysis of nuclei-enriched fractions from
RT   Arabidopsis thaliana.";
RL   J. Proteomics 72:439-451(2009).
RN   [7]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=25409787; DOI=10.1111/tpj.12729;
RA   Berr A., Shafiq S., Pinon V., Dong A., Shen W.;
RT   "The trxG family histone methyltransferase SET DOMAIN GROUP 26
RT   promotes flowering via a distinctive genetic pathway.";
RL   Plant J. 81:316-328(2015).
CC   -!- FUNCTION: Histone methyltransferase involved in regulation of
CC       flowering time. Required for the expression of the SOC1/AGL20
CC       gene. Required for histone H3 trimethylation on 'Lys-4' (H3K4me3)
CC       at the SOC1 locus. Prevents trimethylation on 'Lys-27' (H3K27me3)
CC       at the same locus. {ECO:0000269|PubMed:25409787}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-lysyl-[histone] + S-adenosyl-L-methionine = H(+) +
CC         N(6)-methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:10024, Rhea:RHEA-COMP:9845, Rhea:RHEA-COMP:9846,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:61929; EC=2.1.1.43;
CC         Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome,
CC       centromere {ECO:0000250}. Note=Associates with centromeric
CC       constitutive heterochromatin. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Late flowering.
CC       {ECO:0000269|PubMed:25409787}.
CC   -!- SIMILARITY: Belongs to the class V-like SAM-binding
CC       methyltransferase superfamily. Histone-lysine methyltransferase
CC       family. SET2 subfamily. {ECO:0000255|PROSITE-ProRule:PRU00190}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF04434.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AC010718; AAF04434.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE35879.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE35880.1; -; Genomic_DNA.
DR   EMBL; BT001913; AAN71912.1; -; mRNA.
DR   EMBL; AB493538; BAH30376.1; -; mRNA.
DR   EMBL; AF408059; AAL01110.1; -; mRNA.
DR   PIR; E96795; E96795.
DR   RefSeq; NP_177797.2; NM_106321.4.
DR   RefSeq; NP_974158.1; NM_202429.2.
DR   UniGene; At.70058; -.
DR   ProteinModelPortal; Q84WW6; -.
DR   SMR; Q84WW6; -.
DR   BioGrid; 29224; 7.
DR   IntAct; Q84WW6; 4.
DR   STRING; 3702.AT1G76710.2; -.
DR   iPTMnet; Q84WW6; -.
DR   PaxDb; Q84WW6; -.
DR   EnsemblPlants; AT1G76710.1; AT1G76710.1; AT1G76710.
DR   EnsemblPlants; AT1G76710.2; AT1G76710.2; AT1G76710.
DR   GeneID; 844005; -.
DR   Gramene; AT1G76710.1; AT1G76710.1; AT1G76710.
DR   Gramene; AT1G76710.2; AT1G76710.2; AT1G76710.
DR   KEGG; ath:AT1G76710; -.
DR   Araport; AT1G76710; -.
DR   TAIR; locus:2030131; AT1G76710.
DR   eggNOG; KOG4442; Eukaryota.
DR   eggNOG; COG2940; LUCA.
DR   HOGENOM; HOG000034097; -.
DR   InParanoid; Q84WW6; -.
DR   OrthoDB; 875190at2759; -.
DR   PhylomeDB; Q84WW6; -.
DR   PRO; PR:Q84WW6; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q84WW6; baseline and differential.
DR   Genevisible; Q84WW6; AT.
DR   GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR   GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0046975; F:histone methyltransferase activity (H3-K36 specific); IBA:GO_Central.
DR   GO; GO:0006281; P:DNA repair; IMP:TAIR.
DR   GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR   GO; GO:0016571; P:histone methylation; IDA:TAIR.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0010224; P:response to UV-B; IEP:TAIR.
DR   GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IMP:TAIR.
DR   InterPro; IPR006560; AWS_dom.
DR   InterPro; IPR003616; Post-SET_dom.
DR   InterPro; IPR001214; SET_dom.
DR   Pfam; PF17907; AWS; 1.
DR   Pfam; PF00856; SET; 1.
DR   SMART; SM00570; AWS; 1.
DR   SMART; SM00508; PostSET; 1.
DR   SMART; SM00317; SET; 1.
DR   PROSITE; PS51215; AWS; 1.
DR   PROSITE; PS50868; POST_SET; 1.
DR   PROSITE; PS50280; SET; 1.
PE   1: Evidence at protein level;
KW   Centromere; Chromatin regulator; Chromosome; Complete proteome;
KW   Flowering; Methyltransferase; Nucleus; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    492       Histone-lysine N-methyltransferase ASHH1.
FT                                /FTId=PRO_0000233370.
FT   DOMAIN       36     87       AWS. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00562}.
FT   DOMAIN       84    206       SET. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00190}.
FT   DOMAIN      213    229       Post-SET. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00155}.
SQ   SEQUENCE   492 AA;  55285 MW;  87795D3EAAB01C5D CRC64;
     MQFSCDPDQE GDELPQYEHI YQNDFSYRKH KKQKEEDISI CECKFDFGDP DSACGERCLN
     VITNTECTPG YCPCGVYCKN QKFQKCEYAK TKLIKCEGRG WGLVALEEIK AGQFIMEYCG
     EVISWKEAKK RAQTYETHGV KDAYIISLNA SEAIDATKKG SLARFINHSC RPNCETRKWN
     VLGEVRVGIF AKESISPRTE LAYDYNFEWY GGAKVRCLCG AVACSGFLGA KSRGFQEDTY
     VWEDGDDRYS VDKIPVYDSA EDELTSEPSK NGESNTNEEK EKDISTENHL ESTALNIQQQ
     SDSTPTPMEE DVVTETVKTE TSEDMKLLSQ NSQEDSSPKT AIVSRVHGNI SKIKSESLPK
     KRGRPFSGGK TKNVAQKHVD IANVVQLLAT KEAQDEVLKY EEVKKEAAVR LSSLYDEIRP
     AIEEHERDSQ DSVATSVAEK WIQASCNKLK AEFDLYSSVI KNIASTPIKP QDTKTKVAEA
     GNEDHIKLLE AK
//
DBGET integrated database retrieval system