ID Q883R2_PSESM Unreviewed; 375 AA.
AC Q883R2;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 123.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369};
GN Name=prpC {ECO:0000313|EMBL:AAO55802.1};
GN OrderedLocusNames=PSPTO_2288 {ECO:0000313|EMBL:AAO55802.1};
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283 {ECO:0000313|EMBL:AAO55802.1, ECO:0000313|Proteomes:UP000002515};
RN [1] {ECO:0000313|EMBL:AAO55802.1, ECO:0000313|Proteomes:UP000002515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000 {ECO:0000313|Proteomes:UP000002515};
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., Deboy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S., Brinkac L., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.,
RA Zafar N., Zhou L., Liu J., Yuan Q., Khouri H., Fedorova N., Tran B.,
RA Russell D., Berry K., Utterback T., Van Aken S.E., Feldblyum T.V.,
RA D'Ascenzo M., Deng W.L., Ramos A.R., Alfano J.R., Cartinhour S.,
RA Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B.,
RA Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU003406}.
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DR EMBL; AE016853; AAO55802.1; -; Genomic_DNA.
DR RefSeq; NP_792107.1; NC_004578.1.
DR RefSeq; WP_011103932.1; NC_004578.1.
DR AlphaFoldDB; Q883R2; -.
DR STRING; 223283.PSPTO_2288; -.
DR GeneID; 1183939; -.
DR KEGG; pst:PSPTO_2288; -.
DR PATRIC; fig|223283.9.peg.2323; -.
DR eggNOG; COG0372; Bacteria.
DR HOGENOM; CLU_025068_2_1_6; -.
DR OrthoDB; 9800864at2; -.
DR PhylomeDB; Q883R2; -.
DR UniPathway; UPA00223; UER00717.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0036440; F:citrate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016833; F:oxo-acid-lyase activity; ISS:JCVI.
DR GO; GO:0006113; P:fermentation; ISS:JCVI.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06108; Ec2MCS_like; 1.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR011278; 2-MeCitrate/Citrate_synth_II.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR NCBIfam; TIGR01800; cit_synth_II; 1.
DR PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR11739:SF25; CITRATE SYNTHASE-RELATED PROTEIN DDB_G0287281; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000002515};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369}.
FT ACT_SITE 261
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 312
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 375 AA; 41525 MW; 69D1A6314F9E6C33 CRC64;
MAEAKVLSGA GLRGQVAGQT ALSTVGMAGA GLTYRGYDVR ELAAEAQFEE VAYLLLYGEL
PTQSQLAAYL GKLKGLRDLP QALKEVLERI PADTHPMDVM RTGASMLGTL EPELSFDQQR
DATDRLLAAF PAIMCYWYRF SHDGQRIDCT TDESSIGGHF LHLLLDKTPS ELHRKVMDVS
LILYAEHEFN ASTFTARVCA STLSDLYSCV TAAIGTLRGP LHGGANEAAM EMIQRFASAD
EATQGTLGML ERKEKIMGFG HAIYKESDPR NEVIKGWSKK LADEAGDSVL FPVSEAIDKV
MWEQKKLFPN ADFYHASAYH FMGIPTKLFT PIFVCSRLTG WAAHVFEQRA NNRIIRPSAE
YIGVEQRSFV PIEQR
//
