ID PRMB_BRADU Reviewed; 319 AA.
AC Q89DG5;
DT 14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=Ribosomal protein uL3 glutamine methyltransferase {ECO:0000255|HAMAP-Rule:MF_02125};
DE Short=uL3 MTase {ECO:0000255|HAMAP-Rule:MF_02125};
DE EC=2.1.1.298 {ECO:0000255|HAMAP-Rule:MF_02125};
DE AltName: Full=N5-glutamine methyltransferase PrmB {ECO:0000255|HAMAP-Rule:MF_02125};
GN Name=prmB {ECO:0000255|HAMAP-Rule:MF_02125}; OrderedLocusNames=blr7474;
OS Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS NBRC 14792 / USDA 110).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=224911;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
CC -!- FUNCTION: Methylates large ribosomal subunit protein uL3 on a specific
CC glutamine residue. {ECO:0000255|HAMAP-Rule:MF_02125}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-glutaminyl-[ribosomal protein uL3] + S-adenosyl-L-methionine
CC = H(+) + N(5)-methyl-L-glutaminyl-[ribosomal protein uL3] + S-
CC adenosyl-L-homocysteine; Xref=Rhea:RHEA:45020, Rhea:RHEA-COMP:11063,
CC Rhea:RHEA-COMP:11064, ChEBI:CHEBI:15378, ChEBI:CHEBI:30011,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61891;
CC EC=2.1.1.298; Evidence={ECO:0000255|HAMAP-Rule:MF_02125};
CC -!- SIMILARITY: Belongs to the protein N5-glutamine methyltransferase
CC family. PrmB subfamily. {ECO:0000255|HAMAP-Rule:MF_02125}.
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DR EMBL; BA000040; BAC52739.1; -; Genomic_DNA.
DR RefSeq; NP_774114.1; NC_004463.1.
DR RefSeq; WP_011090207.1; NZ_CP011360.1.
DR AlphaFoldDB; Q89DG5; -.
DR SMR; Q89DG5; -.
DR STRING; 224911.AAV28_35055; -.
DR EnsemblBacteria; BAC52739; BAC52739; BAC52739.
DR GeneID; 64027231; -.
DR KEGG; bja:blr7474; -.
DR PATRIC; fig|224911.44.peg.7573; -.
DR eggNOG; COG2890; Bacteria.
DR HOGENOM; CLU_018398_5_1_5; -.
DR InParanoid; Q89DG5; -.
DR OrthoDB; 9800643at2; -.
DR PhylomeDB; Q89DG5; -.
DR Proteomes; UP000002526; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0036009; F:protein-glutamine N-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR HAMAP; MF_02125; L3_methyltr_PrmB; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004556; HemK-like.
DR InterPro; IPR017127; Ribosome_uL3_MTase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR007848; Small_mtfrase_dom.
DR NCBIfam; TIGR00536; hemK_fam; 1.
DR NCBIfam; TIGR03533; L3_gln_methyl; 1.
DR PANTHER; PTHR47806; 50S RIBOSOMAL PROTEIN L3 GLUTAMINE METHYLTRANSFERASE; 1.
DR PANTHER; PTHR47806:SF1; 50S RIBOSOMAL PROTEIN L3 GLUTAMINE METHYLTRANSFERASE; 1.
DR Pfam; PF05175; MTS; 1.
DR PIRSF; PIRSF037167; Mtase_YfcB_prd; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..319
FT /note="Ribosomal protein uL3 glutamine methyltransferase"
FT /id="PRO_0000414177"
SQ SEQUENCE 319 AA; 34701 MW; 66AABE7B98EA3CE7 CRC64;
MARASKKTAR GRAAPKLAKV GRGELVTLID YVRYSVSRFN EAKLAFAHGT TDPVAEAAFL
VCEALHLHPD QFEAFAHARV TAAEGKTLLD LIHQRVTTRK PAAYLVNKIY MRGLPFYVDE
RVIVPRSYIG ELLDSHFGGD GEAGSLIDHP TAVERVLDLC TGSGCLAILA AYHFPNATVD
AVDISKGALE VAARNVGEHG LDERVTLHRG DLFAPLGDNR YDLIITNPPY VDAEGMAALP
PECRAEPKLA FDGGVDGLDV VRRILRDAPE HLTPDGGLIC EIGRGRELVD EAFPELPLLW
LDTEDSEGEV FWIAAADLD
//
