ID Q89LU8_BRADU Unreviewed; 394 AA.
AC Q89LU8;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE SubName: Full=Cystathionine beta lyase {ECO:0000313|EMBL:BAC49710.1};
GN Name=metC {ECO:0000313|EMBL:BAC49710.1};
OS Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS NBRC 14792 / USDA 110).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=224911 {ECO:0000313|EMBL:BAC49710.1, ECO:0000313|Proteomes:UP000002526};
RN [1] {ECO:0000313|Proteomes:UP000002526}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110
RC {ECO:0000313|Proteomes:UP000002526};
RX PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L,L-cystathionine = L-homocysteine + NH4(+) + pyruvate;
CC Xref=Rhea:RHEA:13965, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:58161, ChEBI:CHEBI:58199;
CC Evidence={ECO:0000256|ARBA:ARBA00001535};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|RuleBase:RU362118};
CC -!- SIMILARITY: Belongs to the trans-sulfuration enzymes family.
CC {ECO:0000256|ARBA:ARBA00009077, ECO:0000256|RuleBase:RU362118}.
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DR EMBL; BA000040; BAC49710.1; -; Genomic_DNA.
DR RefSeq; NP_771085.1; NC_004463.1.
DR RefSeq; WP_011087217.1; NZ_CP011360.1.
DR AlphaFoldDB; Q89LU8; -.
DR STRING; 224911.AAV28_19385; -.
DR EnsemblBacteria; BAC49710; BAC49710; BAC49710.
DR GeneID; 64024190; -.
DR KEGG; bja:bll4445; -.
DR PATRIC; fig|224911.44.peg.4214; -.
DR eggNOG; COG0626; Bacteria.
DR HOGENOM; CLU_018986_5_1_5; -.
DR InParanoid; Q89LU8; -.
DR OrthoDB; 9790858at2; -.
DR PhylomeDB; Q89LU8; -.
DR Proteomes; UP000002526; Chromosome.
DR GO; GO:0004121; F:cystathionine beta-lyase activity; IBA:GO_Central.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0019450; P:L-cysteine catabolic process to pyruvate; IBA:GO_Central.
DR GO; GO:0019346; P:transsulfuration; IEA:InterPro.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR InterPro; IPR000277; Cys/Met-Metab_PyrdxlP-dep_enz.
DR InterPro; IPR006233; Cys_b_lyase_bac.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR NCBIfam; TIGR01324; cysta_beta_ly_B; 1.
DR PANTHER; PTHR43500; CYSTATHIONINE BETA-LYASE-RELATED; 1.
DR PANTHER; PTHR43500:SF1; CYSTATHIONINE BETA-LYASE-RELATED; 1.
DR Pfam; PF01053; Cys_Met_Meta_PP; 1.
DR PIRSF; PIRSF001434; CGS; 1.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR PROSITE; PS00868; CYS_MET_METAB_PP; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000313|EMBL:BAC49710.1};
KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898,
KW ECO:0000256|PIRSR:PIRSR001434-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000002526}.
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 210
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|PIRSR:PIRSR001434-2"
SQ SEQUENCE 394 AA; 42667 MW; FC9EFE8C5FA5986E CRC64;
MDSSHPSQQQ AETRLVTSGR DTKAQKGFVN PPVFHGSTVL YPTAEDLHAH RGEFTYGRHG
TPTTRAFQDT LMALEGPQCA GVGIVPSGLS AISTTLLSVL KTGDHILVCD NVYRPSRNFC
NGMLARYGVE TTYFDPLIGA GIDKLFKPNT RAVLVEAPGS QSFEMPDIRA IAEVAHARDA
LVIDDNTWAT PLYHRSLEQG VDISMQAATK YIGGHSDIMF GTISANAKAW PQIAEGIRLL
GVCAGPDDVF LALRGLRTLS VRLAQHHRSG LDMARWLASR PEVARVLHPG LETDPGHAIW
KRDFTGASGL FSIVLKPAPQ KAVDTMLNTL KLFGMGFSWG GFESLAIPFD CDGYRTATKW
APGGPTLRLH IGLESTDDLK ADLDRGFAAL KTAM
//
