ID Q89R92_BRADU Unreviewed; 516 AA.
AC Q89R92;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 89.
DE RecName: Full=diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244};
GN OrderedLocusNames=blr2880 {ECO:0000313|EMBL:BAC48145.1};
OS Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS NBRC 14792 / USDA 110).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=224911 {ECO:0000313|EMBL:BAC48145.1, ECO:0000313|Proteomes:UP000002526};
RN [1] {ECO:0000313|Proteomes:UP000002526}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110
RC {ECO:0000313|Proteomes:UP000002526};
RX PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587}.
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DR EMBL; BA000040; BAC48145.1; -; Genomic_DNA.
DR RefSeq; NP_769520.1; NC_004463.1.
DR RefSeq; WP_011085664.1; NZ_CP011360.1.
DR AlphaFoldDB; Q89R92; -.
DR STRING; 224911.AAV28_11415; -.
DR EnsemblBacteria; BAC48145; BAC48145; BAC48145.
DR GeneID; 64022632; -.
DR KEGG; bja:blr2880; -.
DR PATRIC; fig|224911.44.peg.2504; -.
DR eggNOG; COG1020; Bacteria.
DR HOGENOM; CLU_024186_4_2_5; -.
DR InParanoid; Q89R92; -.
DR OrthoDB; 7440981at2; -.
DR PhylomeDB; Q89R92; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000002526; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008374; F:O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0001666; P:response to hypoxia; IBA:GO_Central.
DR GO; GO:0071731; P:response to nitric oxide; IBA:GO_Central.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IBA:GO_Central.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR NCBIfam; TIGR02946; acyl_WS_DGAT; 1.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000002526};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..308
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 349..500
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 516 AA; 56064 MW; 0488407A70FCAB6A CRC64;
MADGKKLSSL DASFLYLETP EMPMHVGSMA IFRLPDDYRG DFFEDFKAMI VSRLHIAPIL
KARLEKAPLD IDHPSWVEDD QFDIDRHIFR ASLPQPRDRA TLERIVGWMH AKLLNRARPL
WEFYVFEGMK DNEVGLYSKM HHAAIDGGAG AALTNMIYDI SPIPRKVDPP AAGAKPGQEP
RDIAANLLDS YQQLFSQPLD ASAAAKNLQL PRTGKSDIGS ILFDNAMYQI ESAVRFAGNI
PTVLKSVSDV LGKVADPKSR ESLASMVSPP TMLNKTISSE RSFAGVSIPL SRAKALAKQA
GGKLNDVVLA LASGVVRRYL QQYGTLPAKS LTAAVPISLR EEGNTEANNQ VFGMICAIAT
DIDDPKARLE AIIAQSTKSK EMSHPLRALM PQVSNISMLG APIMVQILAL LYSRSNLSDV
LPPAANITVS NVPGPRQTLY AAGAELLHIF PVSISTHGQA LNITVQSYRD QLDFGFIVGA
NIIPHVQVMC DMLPEEFAAL EAAYAPPAAD IKGAAE
//
