ID Q8A6W6_BACTN Unreviewed; 523 AA.
AC Q8A6W6;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
GN OrderedLocusNames=BT_1760 {ECO:0000313|EMBL:AAO76867.1};
OS Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 /
OS CCUG 10774 / NCTC 10582 / VPI-5482 / E50).
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=226186 {ECO:0000313|EMBL:AAO76867.1, ECO:0000313|Proteomes:UP000001414};
RN [1] {ECO:0000313|EMBL:AAO76867.1, ECO:0000313|Proteomes:UP000001414}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC VPI-5482 / E50 {ECO:0000313|Proteomes:UP000001414};
RX PubMed=12663928; DOI=10.1126/science.1080029;
RA Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C.,
RA Hooper L.V., Gordon J.I.;
RT "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis.";
RL Science 299:2074-2076(2003).
RN [2] {ECO:0000313|EMBL:AAO76867.1, ECO:0000313|Proteomes:UP000001414}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC VPI-5482 / E50 {ECO:0000313|Proteomes:UP000001414};
RX PubMed=19321416; DOI=10.1073/pnas.0901529106;
RA Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S.,
RA Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L.,
RA Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C.,
RA Hettich R.L., Gordon J.I.;
RT "Characterizing a model human gut microbiota composed of members of its two
RT dominant bacterial phyla.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009).
RN [3] {ECO:0007829|PDB:6R3R, ECO:0007829|PDB:6R3U}
RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 23-523 IN COMPLEX WITH ZN(2+).
RX PubMed=31186460; DOI=10.1038/s41598-019-44785-0;
RA Ernits K., Eek P., Lukk T., Visnapuu T., Alamae T.;
RT "First crystal structure of an endo-levanase - the BT1760 from a human gut
RT commensal Bacteroides thetaiotaomicron.";
RL Sci. Rep. 9:8443-8443(2019).
RN [4] {ECO:0007829|PDB:7ZNR, ECO:0007829|PDB:7ZNS}
RP X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS).
RX PubMed=37286596; DOI=10.1038/s41586-023-06146-w;
RA White J.B.R., Silale A., Feasey M., Heunis T., Zhu Y., Zheng H.,
RA Gajbhiye A., Firbank S., Basle A., Trost M., Bolam D.N., van den Berg B.,
RA Ranson N.A.;
RT "Outer membrane utilisomes mediate glycan uptake in gut Bacteroidetes.";
RL Nature 618:583-589(2023).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902}.
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DR EMBL; AE015928; AAO76867.1; -; Genomic_DNA.
DR RefSeq; NP_810673.1; NC_004663.1.
DR PDB; 6R3R; X-ray; 1.65 A; A=23-523.
DR PDB; 6R3U; X-ray; 1.90 A; A=23-523.
DR PDB; 7ZNR; X-ray; 2.65 A; A/B=1-523.
DR PDB; 7ZNS; X-ray; 2.30 A; A/B=1-523.
DR PDB; 8A9Y; EM; 3.50 A; E/M=1-523.
DR PDB; 8AA0; EM; 3.20 A; E/M=1-523.
DR PDB; 8AA2; EM; 3.10 A; E/M=1-523.
DR PDBsum; 6R3R; -.
DR PDBsum; 6R3U; -.
DR AlphaFoldDB; Q8A6W6; -.
DR SMR; Q8A6W6; -.
DR STRING; 226186.BT_1760; -.
DR CAZy; GH32; Glycoside Hydrolase Family 32.
DR PaxDb; 226186-BT_1760; -.
DR DNASU; 1075322; -.
DR EnsemblBacteria; AAO76867; AAO76867; BT_1760.
DR KEGG; bth:BT_1760; -.
DR PATRIC; fig|226186.12.peg.1806; -.
DR eggNOG; COG1621; Bacteria.
DR HOGENOM; CLU_042071_0_0_10; -.
DR InParanoid; Q8A6W6; -.
DR OrthoDB; 9759709at2; -.
DR Proteomes; UP000001414; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd08995; GH32_EcAec43-like; 1.
DR InterPro; IPR032507; BT1760-like_C.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43101; BETA-FRUCTOSIDASE; 1.
DR PANTHER; PTHR43101:SF1; BETA-FRUCTOSIDASE; 1.
DR Pfam; PF16346; GH32_BT1760-like_C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:6R3R, ECO:0007829|PDB:6R3U};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:AAO76867.1};
KW Metal-binding {ECO:0007829|PDB:6R3R, ECO:0007829|PDB:6R3U};
KW Reference proteome {ECO:0000313|Proteomes:UP000001414};
KW Signal {ECO:0000256|SAM:SignalP};
KW Zinc {ECO:0007829|PDB:6R3R, ECO:0007829|PDB:6R3U}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..523
FT /note="beta-fructofuranosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004303154"
FT DOMAIN 96..353
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 353..520
FT /note="BT1760-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16346"
FT BINDING 47
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0007829|PDB:6R3R, ECO:0007829|PDB:6R3U"
FT BINDING 405
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0007829|PDB:6R3R, ECO:0007829|PDB:6R3U"
SQ SEQUENCE 523 AA; 59240 MW; 79B2511FBB802C9F CRC64;
MMKNMILPIA FTALIASMTA CSDETDPILT QKNWDGTATY FQSSDEHGFS MYYKPQVGFV
GDPMPFYDPV AKDFKVMYLQ DYRPNPEATY HPIFGVATKD GATYESLGEL ISCGGRDEQD
AAIGTGGTIY NPADKLYYTF YTGNKFKPSS DQNAQVVMVA TSPDFKTWTK NRTFYLKGDT
YGYDKNDFRD PFLFQTEDGV YHMLIATRKN GKGHIAEFTS ADLKEWESAG TFMTMMWDRF
YECPDVFKMG DWWYLIYSEQ ASFMRKVQYF KGRTLEDLKA TTANDAGIWP DNREGMLDSR
AFYAGKTASD GTNRYIWGWC PTRAGNDNGN VGDVEPEWAG NLVAQRLIQH EDGTLTLGVP
DAIDRKYTSA QEVKVMAKDG NMIESGKTYT LGEGASVIFN RLKVHNKISF TVKTASNTDR
FGISFVRGTD SASWYSIHVN ADEGKANFEK DGDDAKYLFD NKFNIPADNE YRVTIYSDQS
VCVTYINDQL SFTNRIYQMQ KNPWSLCCYK GEITVSDVQV STY
//