ID Q8BM07_MOUSE Unreviewed; 364 AA.
AC Q8BM07;
DT 01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2003, sequence version 1.
DT 27-MAR-2024, entry version 148.
DE SubName: Full=Sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 {ECO:0000313|Ensembl:ENSMUSP00000141130.2};
GN Name=Spock1 {ECO:0000313|Ensembl:ENSMUSP00000141130.2,
GN ECO:0000313|MGI:MGI:105371};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|EMBL:BAC30225.1};
RN [1] {ECO:0000313|EMBL:BAC30225.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC30225.1};
RC TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30225.1};
RX PubMed=10349636; DOI=10.1016/S0076-6879(99)03004-9;
RA Carninci P., Hayashizaki Y.;
RT "High-efficiency full-length cDNA cloning.";
RL Methods Enzymol. 303:19-44(1999).
RN [2] {ECO:0000313|EMBL:BAC30225.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC30225.1};
RC TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30225.1};
RX PubMed=11042159; DOI=10.1101/gr.145100;
RA Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M.,
RA Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.;
RT "Normalization and subtraction of cap-trapper-selected cDNAs to prepare
RT full-length cDNA libraries for rapid discovery of new genes.";
RL Genome Res. 10:1617-1630(2000).
RN [3] {ECO:0000313|EMBL:BAC30225.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC30225.1};
RC TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30225.1};
RX PubMed=11076861; DOI=10.1101/gr.152600;
RA Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P.,
RA Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N.,
RA Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A., Yamamoto R.,
RA Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S.,
RA Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y.,
RA Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y.,
RA Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.;
RT "RIKEN integrated sequence analysis (RISA) system--384-format sequencing
RT pipeline with 384 multicapillary sequencer.";
RL Genome Res. 10:1757-1771(2000).
RN [4] {ECO:0000313|EMBL:BAC30225.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC30225.1};
RC TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30225.1};
RX PubMed=11217851; DOI=10.1038/35055500;
RG The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium;
RT "Functional annotation of a full-length mouse cDNA collection.";
RL Nature 409:685-690(2001).
RN [5] {ECO:0000313|EMBL:BAC30225.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC30225.1};
RC TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30225.1};
RA Adachi J., Aizawa K., Akimura T., Arakawa T., Bono H., Carninci P.,
RA Fukuda S., Furuno M., Hanagaki T., Hara A., Hashizume W., Hayashida K.,
RA Hayatsu N., Hiramoto K., Hiraoka T., Hirozane T., Hori F., Imotani K.,
RA Ishii Y., Itoh M., Kagawa I., Kasukawa T., Katoh H., Kawai J., Kojima Y.,
RA Kondo S., Konno H., Kouda M., Koya S., Kurihara C., Matsuyama T.,
RA Miyazaki A., Murata M., Nakamura M., Nishi K., Nomura K., Numazaki R.,
RA Ohno M., Ohsato N., Okazaki Y., Saito R., Saitoh H., Sakai C., Sakai K.,
RA Sakazume N., Sano H., Sasaki D., Shibata K., Shinagawa A., Shiraki T.,
RA Sogabe Y., Tagami M., Tagawa A., Takahashi F., Takaku-Akahira S.,
RA Takeda Y., Tanaka T., Tomaru A., Toya T., Yasunishi A., Muramatsu M.,
RA Hayashizaki Y.;
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:BAC30225.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC30225.1};
RC TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30225.1};
RX PubMed=12466851; DOI=10.1038/nature01266;
RG The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team;
RT "Analysis of the mouse transcriptome based on functional annotation of
RT 60,770 full-length cDNAs.";
RL Nature 420:563-573(2002).
RN [7] {ECO:0000313|EMBL:BAC30225.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC30225.1};
RC TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30225.1};
RG The FANTOM Consortium;
RG Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group);
RT "The Transcriptional Landscape of the Mammalian Genome.";
RL Science 309:1559-1563(2005).
RN [8] {ECO:0000313|EMBL:BAC30225.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC30225.1};
RC TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30225.1};
RX PubMed=16141073; DOI=10.1126/science.1112009;
RG RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium;
RT "Antisense Transcription in the Mammalian Transcriptome.";
RL Science 309:1564-1566(2005).
RN [9] {ECO:0000313|Ensembl:ENSMUSP00000141130.2, ECO:0000313|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000141130.2,
RC ECO:0000313|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [10] {ECO:0000313|Ensembl:ENSMUSP00000141130.2}
RP IDENTIFICATION.
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000141130.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR EMBL; AK039058; BAC30225.1; -; mRNA.
DR RefSeq; NP_001159936.1; NM_001166464.1.
DR RefSeq; NP_001159937.1; NM_001166465.1.
DR ProteomicsDB; 336408; -.
DR Antibodypedia; 26517; 141 antibodies from 23 providers.
DR DNASU; 20745; -.
DR Ensembl; ENSMUST00000187852.7; ENSMUSP00000141130.2; ENSMUSG00000056222.16.
DR GeneID; 20745; -.
DR UCSC; uc007qtc.2; mouse.
DR AGR; MGI:105371; -.
DR CTD; 6695; -.
DR MGI; MGI:105371; Spock1.
DR VEuPathDB; HostDB:ENSMUSG00000056222; -.
DR GeneTree; ENSGT00940000158371; -.
DR OrthoDB; 4174283at2759; -.
DR BioGRID-ORCS; 20745; 0 hits in 78 CRISPR screens.
DR ChiTaRS; Spock1; mouse.
DR Proteomes; UP000000589; Chromosome 13.
DR Bgee; ENSMUSG00000056222; Expressed in medial dorsal nucleus of thalamus and 171 other cell types or tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00104; KAZAL_FS; 1.
DR Gene3D; 3.30.60.30; -; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR019577; SPARC/Testican_Ca-bd-dom.
DR PANTHER; PTHR13866; SPARC OSTEONECTIN; 1.
DR PANTHER; PTHR13866:SF17; TESTICAN-1; 1.
DR Pfam; PF07648; Kazal_2; 1.
DR Pfam; PF10591; SPARC_Ca_bdg; 1.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF100895; Kazal-type serine protease inhibitors; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 1: Evidence at protein level;
KW Proteomics identification {ECO:0007829|MaxQB:Q8BM07,
KW ECO:0007829|PeptideAtlas:Q8BM07};
KW Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..364
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5015099014"
FT DOMAIN 130..181
FT /note="Kazal-like"
FT /evidence="ECO:0000259|PROSITE:PS51465"
SQ SEQUENCE 364 AA; 40995 MW; 7CDDB6B12E569347 CRC64;
MPAIAVLAAA AAAWCFLQVD SRHLDALAGG AALNNANFLD NDQWLSTVSQ YDRDKYWNRF
RDDDYFRNWN PNKPFDQALD PSKDPCLKVK CSPHKVCVTQ DYQTALCVSR KHLLPRQKKG
NVAHKHWLGP SNLVKCKPCP VAQSAMVCGS DGHTYTSKCK LEFHACSTGK SLNSLCDGPC
PCLPEPEPLK PKAEKSACTD KELRNLASRL KDWFGALHED ANRVIKPTSS DPAQGRFDTS
ILPICKDSLG WMFNKLDMNY DLLLDHSEIN AIYLDKYEPC IKPLFNSCDS FKDGKLSNNE
WCYCFQKPAG LPCQNEMNRI QKLSKGKSLL GTTLENGTVP TRLSVHQFLL SLWRSSWVLP
IHRQ
//
