GenomeNet

Database: UniProt
Entry: Q8E0N5
LinkDB: Q8E0N5
Original site: Q8E0N5 
ID   LDHD_STRA5              Reviewed;         330 AA.
AC   Q8E0N5;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   05-DEC-2018, entry version 97.
DE   RecName: Full=D-lactate dehydrogenase;
DE            Short=D-LDH;
DE            EC=1.1.1.28;
DE   AltName: Full=D-specific 2-hydroxyacid dehydrogenase;
GN   Name=ldhD; Synonyms=ldhA; OrderedLocusNames=SAG0695;
OS   Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=208435;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-611 / 2603 V/R;
RX   PubMed=12200547; DOI=10.1073/pnas.182380799;
RA   Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S.N.,
RA   Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D.,
RA   Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Lewis M.R.,
RA   Radune D., Fedorova N.B., Scanlan D., Khouri H.M., Mulligan S.,
RA   Carty H.A., Cline R.T., Van Aken S.E., Gill J., Scarselli M., Mora M.,
RA   Iacobini E.T., Brettoni C., Galli G., Mariani M., Vegni F., Maione D.,
RA   Rinaudo D., Rappuoli R., Telford J.L., Kasper D.L., Grandi G.,
RA   Fraser C.M.;
RT   "Complete genome sequence and comparative genomic analysis of an
RT   emerging human pathogen, serotype V Streptococcus agalactiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:12391-12396(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(R)-lactate + NAD(+) = H(+) + NADH + pyruvate;
CC         Xref=Rhea:RHEA:16369, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16004, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.1.1.28;
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family. {ECO:0000305}.
DR   EMBL; AE009948; AAM99582.1; -; Genomic_DNA.
DR   RefSeq; NP_687710.1; NC_004116.1.
DR   RefSeq; WP_000770070.1; NC_004116.1.
DR   ProteinModelPortal; Q8E0N5; -.
DR   SMR; Q8E0N5; -.
DR   EnsemblBacteria; AAM99582; AAM99582; SAG0695.
DR   GeneID; 1013499; -.
DR   KEGG; sag:SAG0695; -.
DR   PATRIC; fig|208435.3.peg.701; -.
DR   HOGENOM; HOG000136695; -.
DR   KO; K03778; -.
DR   OMA; NAETHHM; -.
DR   BioCyc; SAGA208435:G1FZP-787-MONOMER; -.
DR   Proteomes; UP000000821; Chromosome.
DR   GO; GO:0008720; F:D-lactate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   InterPro; IPR006139; D-isomer_2_OHA_DH_cat_dom.
DR   InterPro; IPR029753; D-isomer_DH_CS.
DR   InterPro; IPR029752; D-isomer_DH_CS1.
DR   InterPro; IPR006140; D-isomer_DH_NAD-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN         1    330       D-lactate dehydrogenase.
FT                                /FTId=PRO_0000075969.
FT   NP_BIND     155    156       NAD. {ECO:0000250|UniProtKB:P30901}.
FT   NP_BIND     206    207       NAD. {ECO:0000250|UniProtKB:P30901}.
FT   NP_BIND     233    235       NAD. {ECO:0000250|UniProtKB:P30901}.
FT   ACT_SITE    235    235       {ECO:0000250|UniProtKB:P26297}.
FT   ACT_SITE    264    264       {ECO:0000250|UniProtKB:P26297}.
FT   ACT_SITE    296    296       Proton donor.
FT                                {ECO:0000250|UniProtKB:P26297}.
FT   BINDING     175    175       NAD. {ECO:0000250|UniProtKB:P26297}.
FT   BINDING     212    212       NAD. {ECO:0000250|UniProtKB:P30901}.
FT   BINDING     259    259       NAD. {ECO:0000250|UniProtKB:P30901}.
SQ   SEQUENCE   330 AA;  36560 MW;  1F1589D42E3C9BF5 CRC64;
     MKLKVFNVRE EEATLAQDWA NRNHVELSMS EGPLTLETVN EVEGFDGIAN AQIEPLDDAI
     YPLLKEMGIK QIAQRSAGVD MYNLELAKQH GIIISNVPSY SPESIAEFTV TIALNLIRKV
     ELIRANVREQ NFSWTLPIRG RVLGNMTVAI IGTGRIGLAT AKIFKGFGCR VIGYDIYHNP
     MADGILEYVN SVEEAVEEAD LVSLHMPPTA ENTHLFNLDM FKQFKKGAIL MNMARGALVE
     TKDLLEALDQ GLLEGAGIDT YEFEGPYIPK NCQGQDISDK DFLRLINHPK VIYTPHAAYY
     TDEAVKNLVE GALNACVEVI ETGTTTTKVN
//
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