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Database: UniProt
Entry: Q8E5T9
LinkDB: Q8E5T9
Original site: Q8E5T9 
ID   ADDA_STRA3              Reviewed;        1207 AA.
AC   Q8E5T9;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   10-OCT-2018, entry version 95.
DE   RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE   AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   Name=addA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   OrderedLocusNames=gbs0891;
OS   Streptococcus agalactiae serotype III (strain NEM316).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=211110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEM316;
RX   PubMed=12354221; DOI=10.1046/j.1365-2958.2002.03126.x;
RA   Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L.,
RA   Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P.,
RA   Kunst F.;
RT   "Genome sequence of Streptococcus agalactiae, a pathogen causing
RT   invasive neonatal disease.";
RL   Mol. Microbiol. 45:1499-1513(2002).
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA
CC       helicase and an ATP-dependent, dual-direction single-stranded
CC       exonuclease. Recognizes the chi site generating a DNA molecule
CC       suitable for the initiation of homologous recombination. The AddA
CC       nuclease domain is required for chi fragment generation; this
CC       subunit has the helicase and 3' -> 5' nuclease activities.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC       Rule:MF_01451}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
DR   EMBL; AL766847; CAD46535.1; -; Genomic_DNA.
DR   RefSeq; WP_000143226.1; NC_004368.1.
DR   ProteinModelPortal; Q8E5T9; -.
DR   SMR; Q8E5T9; -.
DR   EnsemblBacteria; CAD46535; CAD46535; CAD46535.
DR   KEGG; san:gbs0891; -.
DR   eggNOG; ENOG4107R2I; Bacteria.
DR   eggNOG; COG1074; LUCA.
DR   HOGENOM; HOG000285114; -.
DR   KO; K16898; -.
DR   OMA; KQSIYRW; -.
DR   Proteomes; UP000000823; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.320.10; -; 1.
DR   HAMAP; MF_01451; AddA; 1.
DR   InterPro; IPR014152; DNA_helicase_suAddA.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR011604; Exonuc_phg/RecB_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   PANTHER; PTHR11070:SF14; PTHR11070:SF14; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 3.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding;
KW   Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding.
FT   CHAIN         1   1207       ATP-dependent helicase/nuclease subunit
FT                                A.
FT                                /FTId=PRO_0000379327.
FT   DOMAIN       26    482       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   DOMAIN      510    794       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   NP_BIND      47     54       ATP. {ECO:0000255|HAMAP-Rule:MF_01451}.
SQ   SEQUENCE   1207 AA;  139387 MW;  7CC9A91D88356907 CRC64;
     MTFKPFLNPE DIAVIQTEEK NSDKKQKRTP EQIEAIYTFG NNVLVSASAG SGKTFVMVER
     ILDKLLRGVP IDSLFISTFT VKAAGELKER LEKKINESLK SAESDDLKQF LTQQLVGIQT
     ADIGTMDAFT QKIVNQYGYT LGISPIFRIL QDKNEQDVIK NEVYADLFSD YMTGKNAASF
     IKLVKNFSGN RKDSKAFREM VYKVYAFSQS TDNPKRWMQT VFLKGAQTYT DFEAIPDQEV
     SSLLNVMQTT ANQLRDLTDQ EDYKQLTAKG VPTANYKKHL KIIENLVHWS QDFNLLYGKK
     GLANLARDIT NVIPSGNDVT VAGVKYPIFK QLHNRIVGLK HLEVIFKYQG ESLFLLELLQ
     SFVLDFSEQY LQEKIQENAF EFSDIAHFAI QILEENHDIR QLYQDKYHEV MVDEYQDNNH
     TQERMLELLS NGHNRFMVGD IKQSIYRFRQ ADPQIFNDKY KAYQDNPSQG KLIILKENFR
     SQSEVLDSTN SVFTHLMDEE VGDILYDESH QLKAGSPRQQ ERHPNNKTQV LLLDTDEDDI
     NDSDSQQYDI SPAEAKLVAK EIIRLHKEEN VPFQDITLLV SSRTRNDGIL QTFDRYGIPL
     VTDGGEQNYL KSVEVMVMLD TLRSIDNPLN DYALVALLRS PMFGFNEDDL TRIAIQDVKM
     AFYHKVKLSY HKEGHHSDLI TPELSSKIDH FMKTFQTWRD FAKWHSLYDL IWKIYNDRFY
     YDYVGALPKA EQRQANLYAL ALRANQFEKT GFKGLSRFIR MIDKVLENEN DLADVEVALP
     QNAVNLMTIH KSKGLEFKYV FILNIDKKFS MVDITSPLIL SRNQGIGIKY VADMRHELEE
     ELLPAVKVSM ETLPYQLNKR ELRLATLSEQ MRLLYVAMTR AEKKLYLVGK ASQTKWADHY
     DLVSENNHLP LASRETFVTF QDWLLAVHET YKKQELFYDI NFVSLEELTD HHIGMVNPSL
     PFNPDNKAEN RQSEDIVRAI SVLESVEQIN QTYKAAIELP TVRTPSQVKK FYEPILDIEG
     VDVMETITKT SVDFKLPDFS TSKKQDPAAL GSAVHELMQR IEMSSHVKME DIQKALTEVN
     AETSVKAAIQ IEKINYFFQE TSLGKYIQEE VEHLHREAPF AMLKEDPESG EKFVVRGIID
     GYLLLENRII LFDYKTDKFV NPLELKERYQ GQMALYAEAL KKSYEIEKID KYLILLGGKQ
     LEVVKMD
//
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