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Database: UniProt
Entry: Q8EJ93
LinkDB: Q8EJ93
Original site: Q8EJ93 
ID   RAPA_SHEON              Reviewed;         968 AA.
AC   Q8EJ93;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   31-JUL-2019, entry version 102.
DE   RecName: Full=RNA polymerase-associated protein RapA {ECO:0000255|HAMAP-Rule:MF_01821};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_01821};
DE   AltName: Full=ATP-dependent helicase HepA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   Name=rapA {ECO:0000255|HAMAP-Rule:MF_01821}; Synonyms=hepA;
GN   OrderedLocusNames=SO_0575;
OS   Shewanella oneidensis (strain MR-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=211586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-1;
RX   PubMed=12368813; DOI=10.1038/nbt749;
RA   Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C.,
RA   Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A.,
RA   Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J.,
RA   Brinkac L.M., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S.,
RA   Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A.,
RA   White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M.,
RA   Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J.,
RA   Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O.,
RA   Venter J.C., Nealson K.H., Fraser C.M.;
RT   "Genome sequence of the dissimilatory metal ion-reducing bacterium
RT   Shewanella oneidensis.";
RL   Nat. Biotechnol. 20:1118-1123(2002).
CC   -!- FUNCTION: Transcription regulator that activates transcription by
CC       stimulating RNA polymerase (RNAP) recycling in case of stress
CC       conditions such as supercoiled DNA or high salt concentrations.
CC       Probably acts by releasing the RNAP, when it is trapped or
CC       immobilized on tightly supercoiled DNA. Does not activate
CC       transcription on linear DNA. Probably not involved in DNA repair.
CC       {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SUBUNIT: Interacts with the RNAP. Has a higher affinity for the
CC       core RNAP than for the holoenzyme. Its ATPase activity is
CC       stimulated by binding to RNAP. {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. RapA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01821}.
DR   EMBL; AE014299; AAN53655.1; -; Genomic_DNA.
DR   RefSeq; NP_716210.1; NC_004347.2.
DR   RefSeq; WP_011070913.1; NC_004347.2.
DR   STRING; 211586.SO_0575; -.
DR   PaxDb; Q8EJ93; -.
DR   EnsemblBacteria; AAN53655; AAN53655; SO_0575.
DR   GeneID; 1168443; -.
DR   KEGG; son:SO_0575; -.
DR   PATRIC; fig|211586.12.peg.555; -.
DR   eggNOG; ENOG4105BZK; Bacteria.
DR   eggNOG; COG0553; LUCA.
DR   HOGENOM; HOG000218482; -.
DR   KO; K03580; -.
DR   OMA; MSILERD; -.
DR   OrthoDB; 291634at2; -.
DR   PhylomeDB; Q8EJ93; -.
DR   BioCyc; SONE211586:G1GMP-545-MONOMER; -.
DR   Proteomes; UP000008186; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   HAMAP; MF_01821; Helicase_RapA; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR023949; Helicase_RapA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022737; RapA_C.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   InterPro; IPR040765; Tudor_1_RapA.
DR   InterPro; IPR040766; Tudor_2_RapA.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF12137; RapA_C; 1.
DR   Pfam; PF00176; SNF2_N; 1.
DR   Pfam; PF18339; Tudor_1_RapA; 1.
DR   Pfam; PF18337; Tudor_RapA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; Complete proteome; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    968       RNA polymerase-associated protein RapA.
FT                                /FTId=PRO_0000207186.
FT   DOMAIN      163    332       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   DOMAIN      491    655       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   NP_BIND     176    183       ATP. {ECO:0000255|HAMAP-Rule:MF_01821}.
FT   MOTIF       278    281       DEAH box.
SQ   SEQUENCE   968 AA;  109262 MW;  543F47B925E5D713 CRC64;
     MPFALGQRWI SDTESELGLG TVVQVEGRMV TVLFPATGEN RMFSRNEAPL TRVIYNPGDT
     VESHEGWSLS VEELTEKDDL VVYHGIHSET GEKVSLRETL LNHNIRFNKP QDRLFAGQID
     RLDRFGIRYQ CQLLRHQLAT SDLLGLQGPR VGLIPHQMWI AHEVGRRYAP RVLLADEVGL
     GKTIEAGLII HQQLLTCRAE RVLIIVPDTL RHQWLVEMLR RFNLRFSVFD EDRCVEAFAD
     HDNPFYTEQL VICSLELLRK KKRLDQALDA DWDLLVVDEA HHLEWTEEAP SRAYQVVEAL
     SEVVPGVLLL TATPDQLGHE SHFARLRLLD PDRFYDYDAF LAEENSYKDV AIAAEALAGE
     SKLSDYAINS LTELLSEKDI APSIRLIQAE GIDSELQQAA RSELLQELLD RHGTGRVLYR
     NSRASVKGFP KRIFNAYPHA MPEQYLTAAR VNDMMGGRKS LEAKAAQALS PEKLYQEFED
     NSASWWKFDP RVDWLIEFLK SHRSKKVLII ASGADTALSL EEALRTREGI QATVFHEGMS
     IIERDKAGAY FAQEEGGAQA LICSEIGSEG RNFQFASHLV LFDLPLNPDL LEQRIGRLDR
     IGQKNDIQIH LPYLQDTAQE RLLQWYHQGL NAFELTCPGG HILFSEFAEE LLNVLVGGDE
     DELTNLLNHT QSRYKELKHA MEQGRDKLLE INSHGGDKAK AIVERLAQSD QDTKLIGSVI
     RLWDIIGVDQ EDKGENSIIL RPSEHMMFPT YPGLHEDGVT VTFDRDTALS RDDIALITQE
     HPLVQTGLDL ITGSDTGTTS VAILKNKALP AGTLFLELIY MADASAPKSS QLYRYLPPTP
     IRILLDKNGN DLSAKVDYTS FDKQLSAVNR HIGSKLVTAS QPILHPLFAK GEEYAQVAVN
     ELVAQAREKM TSQLTGELER LESLKAVNPN IREEELEYLR NQMQELTTYL DASQLQLDAI
     RMVLVSHV
//
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