ID Q8ET57_OCEIH Unreviewed; 721 AA.
AC Q8ET57;
DT 01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2003, sequence version 1.
DT 27-MAR-2024, entry version 107.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN OrderedLocusNames=OB0405 {ECO:0000313|EMBL:BAC12361.1};
OS Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC
OS 3954 / HTE831).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Oceanobacillus.
OX NCBI_TaxID=221109 {ECO:0000313|EMBL:BAC12361.1, ECO:0000313|Proteomes:UP000000822};
RN [1] {ECO:0000313|EMBL:BAC12361.1, ECO:0000313|Proteomes:UP000000822}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831
RC {ECO:0000313|Proteomes:UP000000822};
RX PubMed=12235376; DOI=10.1093/nar/gkf526;
RA Takami H., Takaki Y., Uchiyama I.;
RT "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge
RT and its unexpected adaptive capabilities to extreme environments.";
RL Nucleic Acids Res. 30:3927-3935(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; BA000028; BAC12361.1; -; Genomic_DNA.
DR RefSeq; WP_011064811.1; NC_004193.1.
DR AlphaFoldDB; Q8ET57; -.
DR SMR; Q8ET57; -.
DR STRING; 221109.gene:10732608; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR KEGG; oih:OB0405; -.
DR eggNOG; COG1523; Bacteria.
DR HOGENOM; CLU_004744_4_1_9; -.
DR OrthoDB; 9761875at2; -.
DR PhylomeDB; Q8ET57; -.
DR Proteomes; UP000000822; Chromosome.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Glycosidase {ECO:0000313|EMBL:BAC12361.1};
KW Hydrolase {ECO:0000313|EMBL:BAC12361.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000822}.
FT DOMAIN 239..606
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 721 AA; 82838 MW; FF6A977A42DEC36A CRC64;
MTDSIIAWYD DVHRLIIEHP SSNFLNKPNT PVRITERSTN THYYNANINH RDEKEIIVEL
DNPLPIGRDL VLVWNELEIP IYTRKITRTK WFEDNFFTNR KLGAFCNNKS TSFSIWSPVA
TAVHLQIKNN FYLLDRNQDG SWSISLDGDF HRTTYIYQIT INDFTQEVID PYAKSVTANS
TEGVIIDLNR TNALNHQRPN KITKFQDAII YELHLRDLST HENSGINNKG NYLGLTESNT
VTNLGYSTGR CYINELGITH VQLLPVNDFA RVDDLHPNNS YNWGYDPLFF QAPEGSYSSN
PTNPLSRINE LKSCIDSCHE DNLSVILDVV YNHVFQIDHS SFEKMVPGYF FRYHDNGELS
NGTGVGNDFA SEKLMARKFI LDTIDYFLNE YNVDGFRFDL MGAIDVTTMQ EIQKRCQKEK
EFILLLGEGW DLPTALPANQ KSTPNQAHEL TGIAFFNDFF RDTLKGNLFA ADSKGYVNGE
GMHVEHLPHL VTGCVLSKYG IPFAENPGQI VNYVECHDNH TLWDRLSITN SHFTVDERKK
THQLATAITI LSQGIPFLHA GQEWYRTKHG DENSYQSGDN INQLNWSQRE TEEESIAFVR
TLIELRKRYP VFRLPSVEDI NNYVHILPTI HPVFGYTLLD NDADITIYIN PTNTCYDLQL
PSSANWKVAV SNEFIKRNEQ VVIGEQTTLH PYEILVLVKQ RSHQKAKNHA IKSYVSQEKG
Q
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