ID Q8G6U7_BIFLO Unreviewed; 508 AA.
AC Q8G6U7;
DT 01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2003, sequence version 1.
DT 27-MAR-2024, entry version 113.
DE SubName: Full=Sucrose phosphorylase {ECO:0000313|EMBL:AAN24362.1};
GN Name=spl {ECO:0000313|EMBL:AAN24362.1};
GN OrderedLocusNames=BL0536 {ECO:0000313|EMBL:AAN24362.1};
OS Bifidobacterium longum (strain NCC 2705).
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=206672 {ECO:0000313|EMBL:AAN24362.1, ECO:0000313|Proteomes:UP000000439};
RN [1] {ECO:0000313|EMBL:AAN24362.1, ECO:0000313|Proteomes:UP000000439}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCC 2705 {ECO:0000313|Proteomes:UP000000439};
RX PubMed=12381787; DOI=10.1073/pnas.212527599;
RA Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G.,
RA Zwahlen M.C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.;
RT "The genome sequence of Bifidobacterium longum reflects its adaptation to
RT the human gastrointestinal tract.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. Sucrose
CC phosphorylase subfamily. {ECO:0000256|ARBA:ARBA00008452}.
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DR EMBL; AE014295; AAN24362.1; -; Genomic_DNA.
DR RefSeq; NP_695726.1; NC_004307.2.
DR RefSeq; WP_011068532.1; NC_004307.2.
DR AlphaFoldDB; Q8G6U7; -.
DR STRING; 206672.BL0536; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR EnsemblBacteria; AAN24362; AAN24362; BL0536.
DR KEGG; blo:BL0536; -.
DR PATRIC; fig|206672.9.peg.1276; -.
DR HOGENOM; CLU_021358_1_0_11; -.
DR OrthoDB; 9805159at2; -.
DR PhylomeDB; Q8G6U7; -.
DR Proteomes; UP000000439; Chromosome.
DR GO; GO:0004645; F:1,4-alpha-oligoglucan phosphorylase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11355; AmyAc_Sucrose_phosphorylase; 1.
DR Gene3D; 2.20.220.10; alpha-Amylases; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR InterPro; IPR015325; Suc_Porlyase_C.
DR InterPro; IPR016377; Sucrose_GGa_phosphorylase-rel.
DR InterPro; IPR022527; Sucrose_phospho.
DR NCBIfam; TIGR03852; sucrose_gtfA; 1.
DR PANTHER; PTHR38784; SUCROSE PHOSPHORYLASE; 1.
DR PANTHER; PTHR38784:SF1; SUCROSE PHOSPHORYLASE; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF09244; Suc_Porlyase_C; 1.
DR PIRSF; PIRSF003059; Sucrose_phosphorylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Reference proteome {ECO:0000313|Proteomes:UP000000439};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..430
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 192
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-1"
FT ACT_SITE 232
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-1"
FT BINDING 50
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 88
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 190..192
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 232
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 289..290
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 342..345
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 399
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
SQ SEQUENCE 508 AA; 56416 MW; DEF4ED8760410970 CRC64;
MKNKVQLITY ADRLGDGTLS SMTDILRTRF DGVYDGVHIL PFFTPFDGAD AGFDPIDHTK
VDERLGSWDD VAELSKTHNI MVDAIVNHMS WESKQFQDVL EKGEESEYYP MFLTMSSVFP
NGATEEDLAG IYRPRPGLPF THYKFAGKTR LVWVSFTPQQ VDIDTDSDEG WEYLMSIFDQ
MAASHVSYIR LDAVGYGAKE ASTSCFMTPK TFKLISRLRE EGVKRGLEIL IEVHSYYKKQ
VEIASKVDRV YDFALPPLLL HSLFTGHVEP VAHWTEIRPN NAVTVLDTHD GIGVIDIGSD
QLDRSLKGLV PDEDVDNLVN TIHANTHGES QAATGAAASN LDLYQVNSTY YSALGCNDQH
YLAARAVQFF LPGVPQVYYV GALAGRNDME LLRRTNNGRD INRHYYSTAE IDENLERPVV
KALNALAKFR NELSAFDGEF SYEVDGDTSI TFRWTAADGA STAALTFEPG RGLGTDNATP
VASLAWSDAA GDHETHDLLA NPPIADID
//
