ID Q8I3Z9_PLAF7 Unreviewed; 839 AA.
AC Q8I3Z9;
DT 01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2003, sequence version 1.
DT 27-MAR-2024, entry version 144.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
GN ORFNames=PF3D7_0510500 {ECO:0000313|EMBL:CAD51470.1};
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329 {ECO:0000313|EMBL:CAD51470.1, ECO:0000313|Proteomes:UP000001450};
RN [1] {ECO:0000313|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate 3D7 {ECO:0000313|Proteomes:UP000001450};
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.,
RA Eisen J.A., Rutherford K., Salzberg S.L., Craig A., Kyes S., Chan M.S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2] {ECO:0000313|EMBL:CAD51470.1, ECO:0000313|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate 3D7 {ECO:0000313|Proteomes:UP000001450};
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C., Harris B., Harris D.,
RA Mungall K., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corto C., Cronin A., Davies R.,
RA Davies P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James D.,
RA Johnson D., Kerhornou A., Knight A., Kontfortov B., Keyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., McLean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M-A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IB topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00006645}.
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DR EMBL; AL844504; CAD51470.1; -; Genomic_DNA.
DR PIR; S54174; S54174.
DR RefSeq; XP_001351663.1; XM_001351627.1.
DR AlphaFoldDB; Q8I3Z9; -.
DR SMR; Q8I3Z9; -.
DR STRING; 36329.Q8I3Z9; -.
DR PaxDb; 5833-PFE0520c; -.
DR EnsemblProtists; CAD51470; CAD51470; PF3D7_0510500.
DR GeneID; 812833; -.
DR KEGG; pfa:PF3D7_0510500; -.
DR VEuPathDB; PlasmoDB:PF3D7_0510500; -.
DR HOGENOM; CLU_009193_1_2_1; -.
DR InParanoid; Q8I3Z9; -.
DR OMA; HRWKEVK; -.
DR OrthoDB; 10940at2759; -.
DR PhylomeDB; Q8I3Z9; -.
DR Reactome; R-PFA-4615885; SUMOylation of DNA replication proteins.
DR Proteomes; UP000001450; Chromosome 5.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; ISS:GeneDB.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0006265; P:DNA topological change; ISS:GeneDB.
DR CDD; cd03490; Topoisomer_IB_N_1; 1.
DR Gene3D; 1.10.132.10; -; 1.
DR Gene3D; 2.170.11.10; DNA Topoisomerase I, domain 2; 1.
DR Gene3D; 1.10.10.41; Yeast DNA topoisomerase - domain 1; 1.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013034; DNA_topo_DNA_db_N_dom1.
DR InterPro; IPR013030; DNA_topo_DNA_db_N_dom2.
DR InterPro; IPR001631; TopoI.
DR InterPro; IPR018521; TopoI_AS.
DR InterPro; IPR025834; TopoI_C_dom.
DR InterPro; IPR014711; TopoI_cat_a-hlx-sub_euk.
DR InterPro; IPR014727; TopoI_cat_a/b-sub_euk.
DR InterPro; IPR013500; TopoI_cat_euk.
DR InterPro; IPR008336; TopoI_DNA-bd_euk.
DR InterPro; IPR036202; TopoI_DNA-bd_euk_N_sf.
DR InterPro; IPR013499; TopoI_euk.
DR PANTHER; PTHR10290:SF23; DNA TOPOISOMERASE 1; 1.
DR PANTHER; PTHR10290; DNA TOPOISOMERASE I; 1.
DR Pfam; PF14370; Topo_C_assoc; 1.
DR Pfam; PF01028; Topoisom_I; 1.
DR Pfam; PF02919; Topoisom_I_N; 1.
DR PRINTS; PR00416; EUTPISMRASEI.
DR SMART; SM00435; TOPEUc; 1.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR SUPFAM; SSF56741; Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment; 1.
DR PROSITE; PS00176; TOPOISOMERASE_I_EUK; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:CAD51470.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001450};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 308..812
FT /note="DNA topoisomerase I eukaryotic-type"
FT /evidence="ECO:0000259|SMART:SM00435"
FT REGION 27..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 703..730
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 41..106
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 839 AA; 98110 MW; CFAE7D19575B59B6 CRC64;
MQSMEINDNN SIKNESTSDD DILINKIKQN LGNNKSCNSR SSKKESIKKQ KSNSELGIKK
NTKKSLGIKK EEEKKKQISK RKSNELKEKN NLKEGKKKYV EKKSRTVKDE TKLTNVIKKE
TQNNKKPKKL LKKSEENFEP INRWWEKIDD QTDIQWNYLE HRGLIFSPPY VQHHVPIFYK
SIKIELNAKS EELATYWCSA IGSDYCTKEK FILNFFKTFI NSLENDNIIK QENETKLKKG
DISNFKFIDF MPIKDHLLKL REEKLNKTKE EKEEEKKMRM EKELPYTYAL VDWIREKISS
NKAEPPGLFR GRGEHPKQGL LKKRIFPEDV VINISKDAPV PRLYDNMCGH NWGDIYHDNK
VTWLAYYKDS INDQIKYTFL SAQSKFKGYK DLMKYENARK LKSCVHKIRE DYKNKMKNKN
IIDKQLGTAV YLIDFLALRV GGEKDIDEEA DTVGCCSLRV EHISFAHDIP FKSVDSKEQK
TNDEKVNKIP LPTNLESISS EDCYITLDFL GKDSIRYFNT VKIDKQAYIN IIIFCKNKNR
DEGVFDQITC SKLNEYLKEI MPTLSAKVFR TYNASITLDQ QLKRIKEVYG KTTYSLYSGE
TELHKSKKRK SSHLTSDTNI LSDASDSTIN DVNNEYDENG INKKLSYATT VGKENDVDDK
NSPIEVDVSN INELINFYNN ANREVAILCN HQRSIPKQHD TTMSKIKKQI ELYNEDIKEY
KKYLQHLKKN SDKKFIFVSK VSTLDGTLRP NKVKENMKEE SCKKKLITLI KKVELLNNQM
KVRDDNKTIA LGTSKINYMD PRITVAFCKK FEIPIEKVFN RSLRLKFPWA MFATKNFTF
//
