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Database: UniProt
Entry: Q8K3J1
LinkDB: Q8K3J1
Original site: Q8K3J1 
ID   NDUS8_MOUSE             Reviewed;         212 AA.
AC   Q8K3J1;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   13-FEB-2019, entry version 138.
DE   RecName: Full=NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial;
DE            EC=1.6.99.3;
DE            EC=7.1.1.2;
DE   AltName: Full=Complex I-23kD;
DE            Short=CI-23kD;
DE   AltName: Full=NADH-ubiquinone oxidoreductase 23 kDa subunit;
DE   Flags: Precursor;
GN   Name=Ndufs8;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Lucas-Teixeira V.A., Marques S., Belo J.A.;
RT   "Genomic organization of the mouse NDUFS8 gene encoding the NADH
RT   dehydrogenase:ubiquinone Fe-S protein 8.";
RL   Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 19-37; 60-90 AND 118-135, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RA   Lubec G., Kang S.U.;
RL   Submitted (APR-2007) to UniProtKB.
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain;
RX   PubMed=16452087; DOI=10.1074/mcp.T500041-MCP200;
RA   Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,
RA   Burlingame A.L.;
RT   "Comprehensive identification of phosphorylation sites in postsynaptic
RT   density preparations.";
RL   Mol. Cell. Proteomics 5:914-922(2006).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung,
RC   Pancreas, Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and
RT   expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory
CC       chain NADH dehydrogenase (Complex I) that is believed to belong to
CC       the minimal assembly required for catalysis. Complex I functions
CC       in the transfer of electrons from NADH to the respiratory chain.
CC       The immediate electron acceptor for the enzyme is believed to be
CC       ubiquinone (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4
CC         H(+)(out) + NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565,
CC         Rhea:RHEA-COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389,
CC         ChEBI:CHEBI:17976, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=7.1.1.2;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=A + H(+) + NADH = AH2 + NAD(+); Xref=Rhea:RHEA:11356,
CC         ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.6.99.3;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000250};
CC       Note=Binds 2 [4Fe-4S] clusters per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Complex I is composed of 45 different subunits This is a
CC       component of the iron-sulfur (IP) fragment of the enzyme.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I 23 kDa subunit family.
CC       {ECO:0000305}.
DR   EMBL; AY096002; AAM34451.1; -; Genomic_DNA.
DR   CCDS; CCDS29402.1; -.
DR   PIR; PC7079; PC7079.
DR   RefSeq; NP_001258372.1; NM_001271443.1.
DR   RefSeq; NP_001258373.1; NM_001271444.1.
DR   RefSeq; NP_659119.2; NM_144870.5.
DR   UniGene; Mm.44227; -.
DR   PDB; 6G2J; EM; 3.30 A; I=1-212.
DR   PDB; 6G72; EM; 3.90 A; I=1-212.
DR   PDBsum; 6G2J; -.
DR   PDBsum; 6G72; -.
DR   ProteinModelPortal; Q8K3J1; -.
DR   SMR; Q8K3J1; -.
DR   BioGrid; 230440; 1.
DR   ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR   CORUM; Q8K3J1; -.
DR   IntAct; Q8K3J1; 4.
DR   MINT; Q8K3J1; -.
DR   STRING; 10090.ENSMUSP00000074600; -.
DR   iPTMnet; Q8K3J1; -.
DR   PhosphoSitePlus; Q8K3J1; -.
DR   SwissPalm; Q8K3J1; -.
DR   REPRODUCTION-2DPAGE; Q8K3J1; -.
DR   EPD; Q8K3J1; -.
DR   jPOST; Q8K3J1; -.
DR   MaxQB; Q8K3J1; -.
DR   PaxDb; Q8K3J1; -.
DR   PRIDE; Q8K3J1; -.
DR   Ensembl; ENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734.
DR   GeneID; 225887; -.
DR   KEGG; mmu:225887; -.
DR   UCSC; uc008fxn.2; mouse.
DR   CTD; 4728; -.
DR   MGI; MGI:2385079; Ndufs8.
DR   eggNOG; KOG3256; Eukaryota.
DR   eggNOG; COG1143; LUCA.
DR   GeneTree; ENSGT00390000003049; -.
DR   HOGENOM; HOG000228289; -.
DR   HOVERGEN; HBG006547; -.
DR   InParanoid; Q8K3J1; -.
DR   KO; K03941; -.
DR   OMA; LCGLCIE; -.
DR   OrthoDB; 1283957at2759; -.
DR   PhylomeDB; Q8K3J1; -.
DR   TreeFam; TF105610; -.
DR   Reactome; R-MMU-611105; Respiratory electron transport.
DR   Reactome; R-MMU-6799198; Complex I biogenesis.
DR   PRO; PR:Q8K3J1; -.
DR   Proteomes; UP000000589; Chromosome 19.
DR   Bgee; ENSMUSG00000059734; Expressed in 271 organ(s), highest expression level in brown adipose tissue.
DR   ExpressionAtlas; Q8K3J1; baseline and differential.
DR   Genevisible; Q8K3J1; MM.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISO:MGI.
DR   GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:Ensembl.
DR   GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IBA:GO_Central.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISO:MGI.
DR   GO; GO:0006979; P:response to oxidative stress; ISO:MGI.
DR   HAMAP; MF_01351; NDH1_NuoI; 1.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR010226; NADH_quinone_OxRdtase_chainI.
DR   PANTHER; PTHR10849; PTHR10849; 1.
DR   Pfam; PF12838; Fer4_7; 1.
DR   TIGRFAMs; TIGR01971; NuoI; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
PE   1: Evidence at protein level;
KW   3D-structure; 4Fe-4S; Complete proteome; Direct protein sequencing;
KW   Electron transport; Iron; Iron-sulfur; Metal-binding; Mitochondrion;
KW   NAD; Oxidoreductase; Reference proteome; Repeat; Respiratory chain;
KW   Transit peptide; Translocase; Transport; Ubiquinone.
FT   TRANSIT       1     34       Mitochondrion. {ECO:0000250}.
FT   CHAIN        35    212       NADH dehydrogenase [ubiquinone] iron-
FT                                sulfur protein 8, mitochondrial.
FT                                /FTId=PRO_0000020014.
FT   DOMAIN      104    133       4Fe-4S ferredoxin-type 1.
FT   DOMAIN      143    172       4Fe-4S ferredoxin-type 2.
FT   METAL       113    113       Iron-sulfur 1 (4Fe-4S). {ECO:0000250}.
FT   METAL       116    116       Iron-sulfur 1 (4Fe-4S). {ECO:0000250}.
FT   METAL       119    119       Iron-sulfur 1 (4Fe-4S). {ECO:0000250}.
FT   METAL       123    123       Iron-sulfur 2 (4Fe-4S). {ECO:0000250}.
FT   METAL       152    152       Iron-sulfur 2 (4Fe-4S). {ECO:0000250}.
FT   METAL       155    155       Iron-sulfur 2 (4Fe-4S). {ECO:0000250}.
FT   METAL       158    158       Iron-sulfur 2 (4Fe-4S). {ECO:0000250}.
FT   METAL       162    162       Iron-sulfur 1 (4Fe-4S). {ECO:0000250}.
FT   STRAND       36     40       {ECO:0000244|PDB:6G2J}.
FT   HELIX        51     57       {ECO:0000244|PDB:6G2J}.
FT   TURN         58     63       {ECO:0000244|PDB:6G2J}.
FT   HELIX        64     77       {ECO:0000244|PDB:6G2J}.
FT   TURN         86     88       {ECO:0000244|PDB:6G2J}.
FT   STRAND      100    103       {ECO:0000244|PDB:6G2J}.
FT   STRAND      107    109       {ECO:0000244|PDB:6G2J}.
FT   TURN        118    120       {ECO:0000244|PDB:6G2J}.
FT   STRAND      132    134       {ECO:0000244|PDB:6G2J}.
FT   STRAND      136    138       {ECO:0000244|PDB:6G2J}.
FT   STRAND      140    143       {ECO:0000244|PDB:6G2J}.
FT   TURN        157    161       {ECO:0000244|PDB:6G2J}.
FT   STRAND      167    169       {ECO:0000244|PDB:6G2J}.
FT   STRAND      177    180       {ECO:0000244|PDB:6G2J}.
FT   HELIX       181    183       {ECO:0000244|PDB:6G2J}.
FT   HELIX       188    194       {ECO:0000244|PDB:6G2J}.
FT   TURN        195    197       {ECO:0000244|PDB:6G2J}.
FT   HELIX       198    206       {ECO:0000244|PDB:6G2J}.
FT   TURN        207    211       {ECO:0000244|PDB:6G2J}.
SQ   SEQUENCE   212 AA;  24038 MW;  1E06E024EA7829CD CRC64;
     MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI
     LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE HALRRYPSGE ERCIACKLCE
     AICPAQAITI EAEPRADGSR RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFSTETH
     EELLYNKEKL LNNGDKWEAE IAANIQADYL YR
//
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