ID ECFA2_CALS4 Reviewed; 286 AA.
AC Q8R7Y5;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 128.
DE RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE Short=ECF transporter A component EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE EC=3.6.3.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN Name=ecfA2 {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO2;
GN OrderedLocusNames=TTE2260;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Bacillota; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC transporter provides the energy necessary to transport a number of
CC different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR EMBL; AE008691; AAM25404.1; -; Genomic_DNA.
DR RefSeq; WP_011026307.1; NZ_JANUCV010000001.1.
DR PDB; 4MKI; X-ray; 2.30 A; A/B=1-286.
DR PDBsum; 4MKI; -.
DR AlphaFoldDB; Q8R7Y5; -.
DR SMR; Q8R7Y5; -.
DR STRING; 273068.TTE2260; -.
DR KEGG; tte:TTE2260; -.
DR eggNOG; COG1122; Bacteria.
DR HOGENOM; CLU_000604_1_22_9; -.
DR OrthoDB; 9814634at2; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR InterPro; IPR030946; EcfA2.
DR InterPro; IPR030947; EcfA_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR04520; ECF_ATPase_1; 1.
DR NCBIfam; TIGR04521; ECF_ATPase_2; 1.
DR PANTHER; PTHR43553:SF1; ABC TRANSPORTER I FAMILY MEMBER 11, CHLOROPLASTIC; 1.
DR PANTHER; PTHR43553; HEAVY METAL TRANSPORTER; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51246; CBIO; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell membrane; Hydrolase; Membrane;
KW Nucleotide-binding; Reference proteome; Transport.
FT CHAIN 1..286
FT /note="Energy-coupling factor transporter ATP-binding
FT protein EcfA2"
FT /id="PRO_0000092117"
FT DOMAIN 3..244
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT BINDING 40..47
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT STRAND 3..12
FT /evidence="ECO:0007829|PDB:4MKI"
FT STRAND 20..30
FT /evidence="ECO:0007829|PDB:4MKI"
FT STRAND 35..40
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 46..53
FT /evidence="ECO:0007829|PDB:4MKI"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 78..82
FT /evidence="ECO:0007829|PDB:4MKI"
FT STRAND 85..88
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 92..94
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 101..111
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 116..129
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 141..143
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 146..158
FT /evidence="ECO:0007829|PDB:4MKI"
FT STRAND 163..169
FT /evidence="ECO:0007829|PDB:4MKI"
FT TURN 170..173
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 176..193
FT /evidence="ECO:0007829|PDB:4MKI"
FT STRAND 196..200
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 204..210
FT /evidence="ECO:0007829|PDB:4MKI"
FT STRAND 212..218
FT /evidence="ECO:0007829|PDB:4MKI"
FT STRAND 221..226
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 228..232
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 235..240
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 247..257
FT /evidence="ECO:0007829|PDB:4MKI"
FT HELIX 269..282
FT /evidence="ECO:0007829|PDB:4MKI"
SQ SEQUENCE 286 AA; 32136 MW; 414C88AC1B5D706E CRC64;
MPIKVENVSF IYNEGTPYAT VALKDINFSI DDEEFVGIIG HTGSGKSTLI QQLNGLLKPS
KGKIYINGID ITDKKVSLKD IRKQVGLVFQ YPEYQLFEET VFKDIAFGPS NLGLSEEEVK
ERVYEAMEIV GISKELADKS PFELSGGQKR RVAIAGILAM RPKILILDEP TAGLDPKGKQ
EILNKIKEIH DKYKMITILV SHNMEDIARI ADKIIVMNRG KIELIGTPRE VFREAERLEK
IGLSVPQITS LARELRKRGV PIPPDVLTIE EAKEHILRYL RGTKNV
//
