ID Q8R9Q4_CALS4 Unreviewed; 507 AA.
AC Q8R9Q4;
DT 01-JUN-2002, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 107.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN Name=HsdM {ECO:0000313|EMBL:AAM24756.1};
GN OrderedLocusNames=TTE1547 {ECO:0000313|EMBL:AAM24756.1};
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Bacillota; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068 {ECO:0000313|EMBL:AAM24756.1, ECO:0000313|Proteomes:UP000000555};
RN [1] {ECO:0000313|EMBL:AAM24756.1, ECO:0000313|Proteomes:UP000000555}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4
RC {ECO:0000313|Proteomes:UP000000555};
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
RN [2] {ECO:0007829|PDB:5YBB}
RP X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) IN COMPLEX WITH
RP S-ADENOSYL-L-METHIONINE.
RX PubMed=28973912; DOI=10.1073/pnas.1711754114;
RA Liu Y.P., Tang Q., Zhang J.Z., Tian L.F., Gao P., Yan X.X.;
RT "Structural basis underlying complex assembly and conformational transition
RT of the type I R-M system.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:11151-11156(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
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DR EMBL; AE008691; AAM24756.1; -; Genomic_DNA.
DR RefSeq; WP_011025792.1; NZ_JANUCV010000001.1.
DR PDB; 5YBB; X-ray; 3.20 A; A/B/C/E=1-507.
DR PDBsum; 5YBB; -.
DR AlphaFoldDB; Q8R9Q4; -.
DR SMR; Q8R9Q4; -.
DR STRING; 273068.TTE1547; -.
DR REBASE; 6055; M.TteMI.
DR KEGG; tte:TTE1547; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_018284_4_0_9; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF4; TYPE I RESTRICTION ENZYME ECOKI METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:5YBB}; Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AAM24756.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000555};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0007829|PDB:5YBB}; Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 10..135
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 149..455
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 473..504
FT /evidence="ECO:0000256|SAM:Coils"
FT BINDING 173
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:5YBB"
FT BINDING 202
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:5YBB"
FT BINDING 202
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:5YBB"
FT BINDING 233
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:5YBB"
FT BINDING 233
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:5YBB"
FT BINDING 260
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:5YBB"
FT BINDING 260
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:5YBB"
FT BINDING 261
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:5YBB"
FT BINDING 261
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:5YBB"
SQ SEQUENCE 507 AA; 58495 MW; ECEBD3610551EF95 CRC64;
MNGPQTRESL ANEIWRACDI MRRDNNCTGI MEYVEHLAWL LFLRFLDAQE EEWEAQAQIA
GRPYTPIIDS EYRWRHWATK DWPADELLAF VHGRLIPYLR SLGGDPLRET IRSLFSERNV
IVCASGYNLK DVIQIVNEIN FHSQDDIFTV SQVYEELLRR LGNENRLAGE FYTPRPVVRF
VVELVDPQIG EAVYDPACGT CGFLVEAYLW MKQKERTIED HRILQERTFF GQEKKPVPAF
LGLVNMMLHG VTVPRVMRRN TLEENIRNVS ERFDVVVTNP PFGGTEGRHI QQNFPIQSNA
TELLFLQHIM KKLKPRDGAR CGMVVPEGTL FRGGAFAEVK RDLLEQFNLH TVVSLPPGTF
APYSDVKTAL IFFERPGPTK EIWYYELPLP EGLKKFSKGN PIQDEHFEEA RKLWRGWDAY
RKGLGPVEAC LSERSWIVPV EEVKKRGYDL TARNPNRSGG EELPSPVEIV AGLLEKEREI
LSIMEELSEL LENEKGNSSR KNDEEEE
//
