ID Q8SF46_ICTPU Unreviewed; 607 AA.
AC Q8SF46;
DT 01-JUN-2002, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 105.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5 {ECO:0000256|ARBA:ARBA00021096, ECO:0000256|RuleBase:RU003404};
DE EC=7.1.1.2 {ECO:0000256|ARBA:ARBA00012944, ECO:0000256|RuleBase:RU003404};
GN Name=ND5 {ECO:0000313|EMBL:AAL93232.1,
GN ECO:0000313|RefSeq:NP_612135.1};
GN ORFNames=KEF82_p03 {ECO:0000313|RefSeq:NP_612135.1};
OS Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OG Mitochondrion {ECO:0000313|EMBL:AAL93232.1,
OG ECO:0000313|RefSeq:NP_612135.1}.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC Ictaluridae; Ictalurus.
OX NCBI_TaxID=7998 {ECO:0000313|EMBL:AAL93232.1};
RN [1] {ECO:0000313|RefSeq:NP_612135.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Norris {ECO:0000313|RefSeq:NP_612135.1};
RG NCBI Genome Project;
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AAL93232.1, ECO:0000313|RefSeq:NP_612135.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Norris {ECO:0000313|EMBL:AAL93232.1,
RC ECO:0000313|RefSeq:NP_612135.1};
RX PubMed=14631650; DOI=10.1080/1042517031000149057;
RA Waldbieser G.C., Bilodeau A.L., Nonneman D.J.;
RT "Complete sequence and characterization of the channel catfish
RT mitochondrial genome.";
RL DNA Seq. 14:265-277(2003).
RN [3] {ECO:0000313|RefSeq:NP_612135.1}
RP IDENTIFICATION.
RC STRAIN=Norris {ECO:0000313|RefSeq:NP_612135.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000256|RuleBase:RU003404}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000766,
CC ECO:0000256|RuleBase:RU003404};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Mitochondrion
CC inner membrane {ECO:0000256|ARBA:ARBA00004448}; Multi-pass membrane
CC protein {ECO:0000256|ARBA:ARBA00004448}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family.
CC {ECO:0000256|RuleBase:RU003404}.
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DR EMBL; AF482987; AAL93232.1; -; Genomic_DNA.
DR RefSeq; NP_612135.1; NC_003489.1.
DR STRING; 7998.ENSIPUP00000000012; -.
DR Ensembl; ENSIPUT00000000033; ENSIPUP00000000012; ENSIPUG00000000033.
DR GeneID; 804879; -.
DR KEGG; ipu:804879; -.
DR CTD; 4540; -.
DR OMA; GVGIMSF; -.
DR OrthoDB; 1463698at2759; -.
DR Proteomes; UP000221080; Mitochondrion MT.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR010934; NADH_DH_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR NCBIfam; TIGR01974; NDH_I_L; 1.
DR PANTHER; PTHR42829; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5; 1.
DR PANTHER; PTHR42829:SF2; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5; 1.
DR Pfam; PF06455; NADH5_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR PRINTS; PR01434; NADHDHGNASE5.
PE 3: Inferred from homology;
KW Electron transport {ECO:0000256|ARBA:ARBA00022982};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU003404};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128,
KW ECO:0000256|RuleBase:RU003404};
KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|RuleBase:RU003404};
KW Respiratory chain {ECO:0000256|ARBA:ARBA00022660};
KW Signal {ECO:0000256|SAM:SignalP};
KW Translocase {ECO:0000256|ARBA:ARBA00022967};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU003404};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU003404};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU003404};
KW Ubiquinone {ECO:0000256|RuleBase:RU003404}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..607
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5013535984"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 119..136
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 174..193
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 213..236
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 248..269
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 275..297
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 371..389
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 409..431
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 452..471
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 491..510
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT TRANSMEM 585..604
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003404"
FT DOMAIN 70..120
FT /note="NADH-Ubiquinone oxidoreductase (complex I) chain 5
FT N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00662"
FT DOMAIN 136..419
FT /note="NADH:quinone oxidoreductase/Mrp antiporter membrane
FT subunit"
FT /evidence="ECO:0000259|Pfam:PF00361"
FT DOMAIN 425..603
FT /note="NADH dehydrogenase subunit 5 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06455"
SQ SEQUENCE 607 AA; 67289 MW; 03A65DE0E83DF907 CRC64;
MADIMTTTLL LTLAILMWPL MTTLSPTPLD QKWALKYVKT AVSTAFFINT IPLIIFLDQG
TESIITTWNW MNIMNFDINI SFKFDHYSLI FTPIALYVTW SILEFASWYM HSDPYLNRFF
KYLLLFLVAM VILVTANNMF QLFIGWEGVG IMSFLLIGWW HGRADANTAA LQAVIYNRVG
DVGLILTIAW IAMNLNSWEI PQIFLLSKDF DMTLPLMGLI LAATGKSAQF GLHPWLPSAM
EGPTPVSALL HSSTMVVAGI FLLIRLHPLM QDNQLALTVC LCLGALTTLF TATCALTQND
IKKIVAFSTS SRLGLMMVTI GLNQPQLAFL HICTHAFFKA MLFLCSGSII HSLNDEQDIR
KMGGLHKLMP FTSSCLIIGS LALTGMPFLT GFFSKDAIIE ALNTSHLNAW ALALTLIATS
FTAVYSLRVI FFVVMGSPRF STLSPINENH PAVIASIERL AWGSIIAGLI ITSNFLPSKA
PVMTMPLSLK IAAMAVTILG VIIALTLATT TSKQIKITSL LTPHNFSNML GFFPSIIHRS
LPKLNLVLGK QATKLDRQWM EMGPKGLHPL HFTLSSMFDN INTNIIKVYL TFYLISTALI
IVLLSTI
//
