ID Q8TPB3_METAC Unreviewed; 752 AA.
AC Q8TPB3;
DT 01-JUN-2002, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE SubName: Full=Glycogen debranching enzyme {ECO:0000313|EMBL:AAM05403.1};
GN Name=treX {ECO:0000313|EMBL:AAM05403.1};
GN OrderedLocusNames=MA_2000 {ECO:0000313|EMBL:AAM05403.1};
OS Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS C2A).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=188937 {ECO:0000313|EMBL:AAM05403.1, ECO:0000313|Proteomes:UP000002487};
RN [1] {ECO:0000313|EMBL:AAM05403.1, ECO:0000313|Proteomes:UP000002487}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A
RC {ECO:0000313|Proteomes:UP000002487};
RX PubMed=11932238; DOI=10.1101/gr.223902;
RA Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA Cann I., Graham D.E., Grahame D.A., Guss A., Hedderich R., Ingram-Smith C.,
RA Kuettner C.H., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B.,
RA Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H.,
RA de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L.,
RA Paulsen I., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H.,
RA Lander E., Metcalf W.W., Birren B.;
RT "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT physiological diversity.";
RL Genome Res. 12:532-542(2002).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR EMBL; AE010299; AAM05403.1; -; Genomic_DNA.
DR RefSeq; WP_011021995.1; NC_003552.1.
DR AlphaFoldDB; Q8TPB3; -.
DR STRING; 188937.MA_2000; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR EnsemblBacteria; AAM05403; AAM05403; MA_2000.
DR GeneID; 1473889; -.
DR KEGG; mac:MA_2000; -.
DR HOGENOM; CLU_011725_1_1_2; -.
DR InParanoid; Q8TPB3; -.
DR PhylomeDB; Q8TPB3; -.
DR Proteomes; UP000002487; Chromosome.
DR GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR011837; Glycogen_debranch_GlgX.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR NCBIfam; TIGR02100; glgX_debranch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000002487}.
FT DOMAIN 226..631
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 752 AA; 85598 MW; C2A122008CC35641 CRC64;
MKTRSYKIEK GYPHPPGATC DEEGVNFSIY SEHADYVELL LFDKHDDLEP VLVLSTNRVE
ELTRPGKVES LVEASKEIGT SKEIGTSKEI KTSQKIETAE TPGIALNKTF HFWHVYIRGL
KPGVHYAYRI GGPFDPSGGY RFDGGKVLID PYSRGNNKTL WDREKASVPG DNLSTSMRSV
VIDPSRYRWE NNSHITAEKV YEMPGKWEDL FGKSRLQELN ETIIYELHVG GFTRSPSSGV
KTPGTFSGII EKIPYLKELG ITAVELMPVF DFDDTISPEG KRHYWGYDPI CFFAPHSGYC
KNPERGDHTK EFRDMVKALH KAGIEVILDV VFNHTAEGDH LGPVFSFKGI DNSIYYHLEP
DRQYYSNYSG CGNTVNCNHP ISQKLIVDSL KYWTEEMHVD GFRFDQGSIL SLDTDGKVMK
YPPVIWQIEL DDSLGYIKVI AEAWDAAALN QVGYFPGPRW AEWNGYYRDE IRRFVRGDPG
LVGRVASRIA GSPDLYQSES RLPINSVNFV TCHDGFTLND LVSYNYKHNE ANGENNRDGT
DNNLSWNCGV EGETEDPEIE ALRERQIKNF ASILLLSIGV PMISMGDEVR RTQKGNNNAY
CQDNGTGWFD WNLVGRNRDM FRFWKLMIDF RKRHTTILRP RYFTGKENER GLKDISWYGC
KLNSPGWDDP YARALSFTMG EPGDEEDIHV MMNMYWEPLE FEIPGLRERN WHRAVDTFLP
PPQDIAGAGE EVQVNGSSCT VQGRSVVVLI SK
//
