ID GAPN_SCEVA Reviewed; 233 AA.
AC Q8VXQ7;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 22-FEB-2023, entry version 67.
DE RecName: Full=NADP-dependent glyceraldehyde-3-phosphate dehydrogenase;
DE EC=1.2.1.9;
DE AltName: Full=Glyceraldehyde-3-phosphate dehydrogenase [NADP(+)];
DE Short=GAPDHN;
DE AltName: Full=Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase;
DE AltName: Full=Triosephosphate dehydrogenase;
DE Flags: Fragment;
GN Name=GapN;
OS Scenedesmus vacuolatus (Green alga) (Coelastrella vacuolata).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Sphaeropleales; Scenedesmaceae; Scenedesmus.
OX NCBI_TaxID=77546;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC STRAIN=SAG 211-8b;
RX PubMed=15830207; DOI=10.1007/s00425-005-1501-0;
RA Valverde F., Ortega J.M., Losada M., Serrano A.;
RT "Sugar-mediated transcriptional regulation of the Gap gene system and
RT concerted photosystem II functional modulation in the microalga Scenedesmus
RT vacuolatus.";
RL Planta 221:937-952(2005).
CC -!- FUNCTION: Important as a means of generating NADPH for biosynthetic
CC reactions.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glyceraldehyde 3-phosphate + H2O + NADP(+) = (2R)-3-
CC phosphoglycerate + 2 H(+) + NADPH; Xref=Rhea:RHEA:14669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58272, ChEBI:CHEBI:58349, ChEBI:CHEBI:59776; EC=1.2.1.9;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- INDUCTION: Induced by sugar addition. {ECO:0000269|PubMed:15830207}.
CC -!- MISCELLANEOUS: Algae contain three forms of GAPDH: two cytosolic forms
CC which participate in glycolysis and one chloroplastic form which
CC participates in photosynthesis. These three forms are encoded by
CC distinct genes.
CC -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; AJ252211; CAC81014.1; -; mRNA.
DR AlphaFoldDB; Q8VXQ7; -.
DR SMR; Q8VXQ7; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008886; F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity; IEA:UniProtKB-EC.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016163; Ald_DH_C.
DR InterPro; IPR016160; Ald_DH_CS_CYS.
DR InterPro; IPR029510; Ald_DH_CS_GLU.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH_dom.
DR PANTHER; PTHR42991; ALDEHYDE DEHYDROGENASE; 1.
DR PANTHER; PTHR42991:SF1; ALDEHYDE DEHYDROGENASE; 1.
DR Pfam; PF00171; Aldedh; 1.
DR SUPFAM; SSF53720; ALDH-like; 1.
DR PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; NADP; Oxidoreductase.
FT CHAIN <1..>233
FT /note="NADP-dependent glyceraldehyde-3-phosphate
FT dehydrogenase"
FT /id="PRO_0000291764"
FT ACT_SITE 102
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10007,
FT ECO:0000255|PROSITE-ProRule:PRU10008"
FT ACT_SITE 136
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10007,
FT ECO:0000255|PROSITE-ProRule:PRU10008"
FT BINDING 7..8
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 30
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 33
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 83..87
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 135..137
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 180
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT BINDING 229
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT SITE 7
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
FT NON_TER 1
FT NON_TER 233
SQ SEQUENCE 233 AA; 24436 MW; F198D939BB96FD69 CRC64;
LAIPPFNYPV NLAVSKLAPA LMAGNTVVLK PPSQGVVAGI HMIKCFQAAG LPAGTVNLVT
GKGSEIGDFL TTHPAVNCIS FTGGDTGIAI SRKAGMVPLQ MELGGKDACI VCSDADLDLA
ATHIIKGGFS YSGQRCTAVK VVLVMQDIAD ELVRKVHAGV QKLKVGRPED NADITAVVSE
GSANFIQGLV EDAKAKGATF LTDWKREGNL LWPVLLDNVT ADMRIAWEEP FGP
//
