ID AP5M_ARATH Reviewed; 613 AA.
AC Q8W0Z6; Q9SKT8;
DT 13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 24-JAN-2024, entry version 122.
DE RecName: Full=AP-5 complex subunit mu;
DE AltName: Full=Adaptor protein complex AP-5 subunit mu;
DE AltName: Full=Adaptor protein-5 mu-adaptin;
DE AltName: Full=Adaptor-related protein complex 5 subunit mu;
DE AltName: Full=Mu5-adaptin;
GN Name=AP5M; OrderedLocusNames=At2g20790; ORFNames=F5H14.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP REVIEW.
RX PubMed=22022230; DOI=10.1371/journal.pbio.1001170;
RA Hirst J., Barlow L.D., Francisco G.C., Sahlender D.A., Seaman M.N.,
RA Dacks J.B., Robinson M.S.;
RT "The fifth adaptor protein complex.";
RL PLoS Biol. 9:E1001170-E1001170(2011).
CC -!- SUBUNIT: Probably part of the adaptor protein complex 5 (AP-5).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}; Cytoplasmic side
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8W0Z6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8W0Z6-2; Sequence=VSP_053359;
CC -!- SIMILARITY: Belongs to the adaptor complexes medium subunit family.
CC {ECO:0000305}.
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DR EMBL; AC006234; AAD20916.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07071.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07073.1; -; Genomic_DNA.
DR EMBL; AF462850; AAL58937.1; -; mRNA.
DR EMBL; AY090282; AAL90943.1; -; mRNA.
DR PIR; D84593; D84593.
DR RefSeq; NP_850004.1; NM_179673.3. [Q8W0Z6-1]
DR RefSeq; NP_973495.1; NM_201766.2. [Q8W0Z6-2]
DR AlphaFoldDB; Q8W0Z6; -.
DR BioGRID; 1961; 2.
DR IntAct; Q8W0Z6; 2.
DR STRING; 3702.Q8W0Z6; -.
DR iPTMnet; Q8W0Z6; -.
DR PaxDb; 3702-AT2G20790-1; -.
DR ProteomicsDB; 246664; -. [Q8W0Z6-1]
DR EnsemblPlants; AT2G20790.1; AT2G20790.1; AT2G20790. [Q8W0Z6-1]
DR EnsemblPlants; AT2G20790.2; AT2G20790.2; AT2G20790. [Q8W0Z6-2]
DR GeneID; 816608; -.
DR Gramene; AT2G20790.1; AT2G20790.1; AT2G20790. [Q8W0Z6-1]
DR Gramene; AT2G20790.2; AT2G20790.2; AT2G20790. [Q8W0Z6-2]
DR KEGG; ath:AT2G20790; -.
DR Araport; AT2G20790; -.
DR TAIR; AT2G20790; -.
DR eggNOG; KOG0937; Eukaryota.
DR InParanoid; Q8W0Z6; -.
DR OMA; EYILWNT; -.
DR OrthoDB; 677575at2759; -.
DR PhylomeDB; Q8W0Z6; -.
DR PRO; PR:Q8W0Z6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8W0Z6; baseline and differential.
DR Genevisible; Q8W0Z6; AT.
DR GO; GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR CDD; cd09256; AP_MuD_MHD; 1.
DR Gene3D; 2.60.40.1170; Mu homology domain, subdomain B; 1.
DR InterPro; IPR036168; AP2_Mu_C_sf.
DR InterPro; IPR039591; AP5M1.
DR InterPro; IPR018240; Clathrin_mu_CS.
DR InterPro; IPR028565; MHD.
DR PANTHER; PTHR16082; AP-5 COMPLEX SUBUNIT MU-1; 1.
DR PANTHER; PTHR16082:SF2; AP-5 COMPLEX SUBUNIT MU-1; 1.
DR Pfam; PF00928; Adap_comp_sub; 1.
DR SUPFAM; SSF49447; Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor; 1.
DR PROSITE; PS00991; CLAT_ADAPTOR_M_2; 1.
DR PROSITE; PS51072; MHD; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasmic vesicle; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..613
FT /note="AP-5 complex subunit mu"
FT /id="PRO_0000424265"
FT DOMAIN 309..563
FT /note="MHD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00404"
FT REGION 501..522
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..138
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_053359"
SQ SEQUENCE 613 AA; 66705 MW; BD001DA99C54FEBC CRC64;
MPSGCSIRAL WIINNQDTVV FSRRFPVVEK QWCSAYKTEN ENTGLDLPRL PTDQQISDSF
TRRKRREGST RGYGIRVAQS TKGSDSWVDD PITRHIISLC LTEEDDDDDD ERNILWPIAL
HTKALYSILV LPLVEPKEMK DYVKLCRRSD CGPAVGEDLS LSSLLLNISS ITGAFMVAHA
FGDIISGDTV EPEVVVSVSP SVGGLFDSLT GSIGISSRAK PVAAPVASSN PSGAAITGAT
ASDAPKAGSR LLDRDLLRNF IATAMPFGTP LDLSLSNISA MKANGFSSAD PPPQELKQPA
WKPYLYKGKQ RLLFTIHETV SAAMYDRDEI PDNVSVAGQI NCRAELEGLP DVSFPLAGLS
TAHIEAISFH PCAQVPAHGI DKQNIVFQPP LGNFVLMRYQ AGCGLGPPVK GFYQLSMVSE
DEGAFLFKVH LMEGYKAPLS MEFCTITMPF PRRRIVAFDG TPSAGTVLTT EHSVEWRILG
SGRSLSGKSL EATFPGTIKF SPLQSRRKGD GDDEESEDES AENVVNVEDF LVQKMNKDLP
AAELEEPFCW QAYDYAKVSF KIVGASVSRM SIDTKSVNIY PTTKSPVEFS AQVTSGDYIL
WNTLGKAPSA AVV
//
