ID ASZ1_PANTR Reviewed; 475 AA.
AC Q8WMX6; Q2QLE6;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 27-MAR-2024, entry version 125.
DE RecName: Full=Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1;
DE AltName: Full=Germ cell-specific ankyrin, SAM and basic leucine zipper domain-containing protein;
GN Name=ASZ1; Synonyms=GASZ;
OS Pan troglodytes (Chimpanzee).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pan.
OX NCBI_TaxID=9598;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12040005; DOI=10.1210/mend.16.6.0864;
RA Yan W., Rajkovic A., Viveiros M.M., Burns K.H., Eppig J.J., Matzuk M.M.;
RT "Identification of Gasz, an evolutionarily conserved gene expressed
RT exclusively in germ cells and encoding a protein with four ankyrin repeats,
RT a sterile-alpha motif, and a basic leucine zipper.";
RL Mol. Endocrinol. 16:1168-1184(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12917688; DOI=10.1038/nature01858;
RA Thomas J.W., Touchman J.W., Blakesley R.W., Bouffard G.G.,
RA Beckstrom-Sternberg S.M., Margulies E.H., Blanchette M., Siepel A.C.,
RA Thomas P.J., McDowell J.C., Maskeri B., Hansen N.F., Schwartz M.S.,
RA Weber R.J., Kent W.J., Karolchik D., Bruen T.C., Bevan R., Cutler D.J.,
RA Schwartz S., Elnitski L., Idol J.R., Prasad A.B., Lee-Lin S.-Q.,
RA Maduro V.V.B., Summers T.J., Portnoy M.E., Dietrich N.L., Akhter N.,
RA Ayele K., Benjamin B., Cariaga K., Brinkley C.P., Brooks S.Y., Granite S.,
RA Guan X., Gupta J., Haghighi P., Ho S.-L., Huang M.C., Karlins E.,
RA Laric P.L., Legaspi R., Lim M.J., Maduro Q.L., Masiello C.A.,
RA Mastrian S.D., McCloskey J.C., Pearson R., Stantripop S., Tiongson E.E.,
RA Tran J.T., Tsurgeon C., Vogt J.L., Walker M.A., Wetherby K.D.,
RA Wiggins L.S., Young A.C., Zhang L.-H., Osoegawa K., Zhu B., Zhao B.,
RA Shu C.L., De Jong P.J., Lawrence C.E., Smit A.F., Chakravarti A.,
RA Haussler D., Green P., Miller W., Green E.D.;
RT "Comparative analyses of multi-species sequences from targeted genomic
RT regions.";
RL Nature 424:788-793(2003).
CC -!- FUNCTION: Plays a central role during spermatogenesis by repressing
CC transposable elements and preventing their mobilization, which is
CC essential for the germline integrity. Acts via the piRNA metabolic
CC process, which mediates the repression of transposable elements during
CC meiosis by forming complexes composed of piRNAs and Piwi proteins and
CC governs the methylation and subsequent repression of transposons. Its
CC association with pi-bodies suggests a participation in the primary
CC piRNAs metabolic process. Required prior to the pachytene stage to
CC facilitate the production of multiple types of piRNAs, including those
CC associated with repeats involved in the regulation of retrotransposons.
CC May act by mediating protein-protein interactions during germ cell
CC maturation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with DDX4, PIWIL1, RANBP9 and TDRD1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=Component of the
CC meiotic nuage, also named P granule, a germ-cell-specific organelle
CC required to repress transposon activity during meiosis. Specifically
CC localizes to pi-bodies, a subset of the nuage which contains primary
CC piRNAs (By similarity). {ECO:0000250}.
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DR EMBL; AF461263; AAL68819.1; -; mRNA.
DR EMBL; DP000016; AAR16252.1; -; Genomic_DNA.
DR RefSeq; NP_001009035.1; NM_001009035.1.
DR AlphaFoldDB; Q8WMX6; -.
DR SMR; Q8WMX6; -.
DR STRING; 9598.ENSPTRP00000033595; -.
DR PaxDb; 9598-ENSPTRP00000033595; -.
DR Ensembl; ENSPTRT00000036337.4; ENSPTRP00000033595.3; ENSPTRG00000019618.4.
DR GeneID; 450111; -.
DR KEGG; ptr:450111; -.
DR CTD; 136991; -.
DR VGNC; VGNC:7215; ASZ1.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00880000138051; -.
DR HOGENOM; CLU_053259_0_0_1; -.
DR InParanoid; Q8WMX6; -.
DR OMA; SFQYGWT; -.
DR OrthoDB; 5354112at2759; -.
DR TreeFam; TF352216; -.
DR Proteomes; UP000002277; Chromosome 7.
DR Bgee; ENSPTRG00000019618; Expressed in testis.
DR GO; GO:0071546; C:pi-body; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007140; P:male meiotic nuclear division; ISS:UniProtKB.
DR GO; GO:0034587; P:piRNA processing; IBA:GO_Central.
DR GO; GO:0010526; P:retrotransposon silencing; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR CDD; cd09521; SAM_ASZ1; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 1.10.150.50; Transcription Factor, Ets-1; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR042650; Asz1_SAM.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR PANTHER; PTHR24157; ANKYRIN REPEAT, SAM AND BASIC LEUCINE ZIPPER DOMAIN-CONTAINING PROTEIN 1; 1.
DR PANTHER; PTHR24157:SF3; ANKYRIN REPEAT, SAM AND BASIC LEUCINE ZIPPER DOMAIN-CONTAINING PROTEIN 1; 1.
DR Pfam; PF00023; Ank; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF07647; SAM_2; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 5.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF140860; Pseudo ankyrin repeat-like; 1.
DR SUPFAM; SSF47769; SAM/Pointed domain; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 3.
PE 2: Evidence at transcript level;
KW ANK repeat; Cytoplasm; Developmental protein; Differentiation; Meiosis;
KW Phosphoprotein; Reference proteome; Repeat; RNA-mediated gene silencing;
KW Spermatogenesis.
FT CHAIN 1..475
FT /note="Ankyrin repeat, SAM and basic leucine zipper domain-
FT containing protein 1"
FT /id="PRO_0000066971"
FT REPEAT 45..74
FT /note="ANK 1"
FT REPEAT 78..107
FT /note="ANK 2"
FT REPEAT 110..144
FT /note="ANK 3"
FT REPEAT 148..177
FT /note="ANK 4"
FT REPEAT 181..210
FT /note="ANK 5"
FT REPEAT 214..243
FT /note="ANK 6"
FT DOMAIN 272..334
FT /note="SAM"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 17
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8VD46"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8VD46"
FT MOD_RES 20
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8VD46"
SQ SEQUENCE 475 AA; 53319 MW; 4BC6CFE6209347A8 CRC64;
MAASALRGLP VAGGGESSES EDDGWEIGYL DRTSQKLKGL LPIEEKKEKF KKAMTIGDVS
LVQELLDSGI SVDSTFQYGW TPLMYAASVA NAELVRVLLD RGANASFEKD KQSILITACS
AHGSEEQILK CVELLLSRNA DPNVACRRLM TPIMYAARDG HTQVVALLVA HGAEVNTQDE
NGYTALTWAA RQGHKNIVLK LLELGANKML QTKDGKMPSE IAKRNKHHEI FNLLSFTLNP
LEGKLQQLTK EDTICKILTT DSDREKDHIF SSYTAFGDLE VFLHGLGLEH MTDILKERDI
TLRHLLTMRE DEFTKNGITS KDQQKILAAL KELQVEEIQF GELSEETKLE ISGDEFLNFL
LKLNKQCGHL ITAVQNVITE LPVNSQKITL EWASPQNFTS VCEELVNNVE DLSEKVCKLK
DLIQKLQNER ESDPTHIQLR EEVSTWNSRI LKRTAITICG FGFLLFICKL TFQRK
//
