ID Q8X7E1_ECO57 Unreviewed; 373 AA.
AC Q8X7E1; A0A0H3JIX2; A0A6M0JN23; Q7AEJ9;
DT 01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2002, sequence version 1.
DT 27-MAR-2024, entry version 138.
DE SubName: Full=Multidrug-efflux transport protein {ECO:0000313|EMBL:BAB35286.1};
GN Name=eefA {ECO:0000313|EMBL:BAB35286.1};
GN ORFNames=ECs_1863 {ECO:0000313|EMBL:BAB35286.1};
OS Escherichia coli O157:H7.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334 {ECO:0000313|EMBL:BAB35286.1, ECO:0000313|Proteomes:UP000000558};
RN [1] {ECO:0000313|EMBL:BAB35286.1, ECO:0000313|Proteomes:UP000000558}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC
RC {ECO:0000313|Proteomes:UP000000558};
RX PubMed=11258796; DOI=.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004519}; Lipid-anchor
CC {ECO:0000256|ARBA:ARBA00004519}. Membrane
CC {ECO:0000256|ARBA:ARBA00004635}; Lipid-anchor
CC {ECO:0000256|ARBA:ARBA00004635}.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000256|ARBA:ARBA00009477}.
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DR EMBL; BA000007; BAB35286.1; -; Genomic_DNA.
DR RefSeq; NP_309890.1; NC_002695.1.
DR RefSeq; WP_000873809.1; NZ_VOAI01000015.1.
DR AlphaFoldDB; Q8X7E1; -.
DR STRING; 155864.Z2509; -.
DR GeneID; 912781; -.
DR KEGG; ecs:ECs_1863; -.
DR PATRIC; fig|386585.9.peg.1965; -.
DR eggNOG; COG0845; Bacteria.
DR HOGENOM; CLU_018816_2_1_6; -.
DR OMA; DNSKMWV; -.
DR Proteomes; UP000000558; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProt.
DR Gene3D; 2.40.30.170; -; 1.
DR Gene3D; 2.40.420.20; -; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 1.10.287.470; Helix hairpin bin; 1.
DR InterPro; IPR043602; CusB_dom_1.
DR InterPro; IPR032317; HlyD_D23.
DR InterPro; IPR006143; RND_pump_MFP.
DR NCBIfam; TIGR01730; RND_mfp; 1.
DR PANTHER; PTHR30158; ACRA/E-RELATED COMPONENT OF DRUG EFFLUX TRANSPORTER; 1.
DR PANTHER; PTHR30158:SF3; MULTIDRUG EFFLUX PUMP SUBUNIT ACRA-RELATED; 1.
DR Pfam; PF00529; CusB_dom_1; 1.
DR Pfam; PF16576; HlyD_D23; 1.
DR SUPFAM; SSF111369; HlyD-like secretion proteins; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Palmitate {ECO:0000256|ARBA:ARBA00023139};
KW Reference proteome {ECO:0000313|Proteomes:UP000000558};
KW Signal {ECO:0000256|ARBA:ARBA00022729}.
FT DOMAIN 31..349
FT /note="Cation efflux system protein CusB"
FT /evidence="ECO:0000259|Pfam:PF00529"
FT DOMAIN 58..281
FT /note="RND efflux pump membrane fusion protein barrel-
FT sandwich"
FT /evidence="ECO:0000259|Pfam:PF16576"
SQ SEQUENCE 373 AA; 39613 MW; 2A573537AB44CDBF CRC64;
MKYIATSVVA MLLLSGCDNT QSNNSSPSET EVGVVTVKSQ PVSVVSELTG RTSAALSAEV
RPQVGGIIQK RLFKEGDLVK AGQPLYQIDA ASYQAAWNEA RAALQQAQAL VKADCQKAQR
YARLVKENGV SQQDADDAQS TCAQDKASVA AKKAALETAR INLDWTTVTA PISGRIGISS
VTPGALVTAS QDTALTTIRG LDTMYVDLTR SSVDLLRLRK QSLATNSDTM SVSLILEDGT
TYSEKGRLEL TEVAVDESTG SVTLRAIFPN PQQQLLPGMF VRARVDEGVM EDAILAPQQG
VTRDAKGNAT ALVVNKDNKV EQRTLETGET YGDKWLVLNG LHSGDRLIVE GSAKVTSGQT
VKAVEVQANG GNA
//
