GenomeNet

Database: UniProt
Entry: Q8XPE2
LinkDB: Q8XPE2
Original site: Q8XPE2 
ID   ADDA_CLOPE              Reviewed;        1268 AA.
AC   Q8XPE2;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-APR-2018, entry version 97.
DE   RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE   AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   Name=addA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   OrderedLocusNames=CPE0021;
OS   Clostridium perfringens (strain 13 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=195102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=13 / Type A;
RX   PubMed=11792842; DOI=10.1073/pnas.022493799;
RA   Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A.,
RA   Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.;
RT   "Complete genome sequence of Clostridium perfringens, an anaerobic
RT   flesh-eater.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002).
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA
CC       helicase and an ATP-dependent, dual-direction single-stranded
CC       exonuclease. Recognizes the chi site generating a DNA molecule
CC       suitable for the initiation of homologous recombination. The AddA
CC       nuclease domain is required for chi fragment generation; this
CC       subunit has the helicase and 3' -> 5' nuclease activities.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC       Rule:MF_01451}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
DR   EMBL; BA000016; BAB79727.1; -; Genomic_DNA.
DR   RefSeq; WP_011009572.1; NC_003366.1.
DR   ProteinModelPortal; Q8XPE2; -.
DR   SMR; Q8XPE2; -.
DR   EnsemblBacteria; BAB79727; BAB79727; BAB79727.
DR   KEGG; cpe:CPE0021; -.
DR   HOGENOM; HOG000015621; -.
DR   KO; K16898; -.
DR   OMA; KQSIYRW; -.
DR   OrthoDB; POG091H004Y; -.
DR   Proteomes; UP000000818; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006302; P:double-strand break repair; IEA:InterPro.
DR   Gene3D; 3.90.320.10; -; 1.
DR   HAMAP; MF_01451; AddA; 1.
DR   InterPro; IPR014152; DNA_helicase_suAddA.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR011604; Exonuc_phg/RecB_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   PANTHER; PTHR11070:SF14; PTHR11070:SF14; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 4.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding;
KW   Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1   1268       ATP-dependent helicase/nuclease subunit
FT                                A.
FT                                /FTId=PRO_0000379261.
FT   DOMAIN        3    476       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   DOMAIN      528    824       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   NP_BIND      24     31       ATP. {ECO:0000255|HAMAP-Rule:MF_01451}.
SQ   SEQUENCE   1268 AA;  146904 MW;  4631AEE9E0D53234 CRC64;
     MGTKWTEEQE LAINTRKCNL LVAAAAGSGK TAVLVERIIK MITEGENPVD IDKLLVVTFT
     NAAASEMRER IGDAISKALE KDPSSKVLQR QLALLNRASI TTMHSFCLEV IKNNFHLIDL
     DPGFRIGDQT ECELIKQDIL ADLFEDMYAK DDECFKDLVE AYGGSKSDDN LNSIILKFYN
     FIMSGPWPEA WLKDKVEEFN INSIEELEGK KWIEVLKESI ILDLNNAYSM LTQARDIAEM
     GGGLEPYLVN INPEIIQVEE LKIALSEGIV KFYNNLMGAS FGRLKSVRKA SVDDERALEK
     TKSLRDESKK IIENLRDNVF ETSLEEAVLG MKKMYPLMKC LSGLVIEFSN RYRDKKREKD
     ILDFNDLEHL CLEILIDKDE EGNIKPSQVA LEFKDRFEEV LVDEYQDSNT IQETIVGMVS
     RRDIENPNVF MVGDVKQSIY KFRQANPELF LEKYINYREF EDSNRKIMLY KNFRSREEII
     NGVNYIFKTL MSNTVGELEY DEKEALNLGA SYGELNEENV EKEYIDEIEN LKVAGDIELN
     ILNKAGNKDY SDEDELGEEE EDLDSIQLEA RIIGKKINEL MNPEDGSHYM VFDKDLGKYR
     KIKYKDIVIL LRATKNWAET FVDELGTYGI PVYADTGTGY FQTIEIRTIL ALLHIIDNPM
     QDIYILSALR SPIFSFTSEE FADLRLLNKD KYFFEIIKEV VDGIYDESIS KDLKGKCKYF
     LDYLNKWREK AAYMPIDEFI WFLYSDTSYY GYVGTMPNGV QRQANLRILF QRAKQYESTS
     FKGLFNFINF INKLKKSSGD MGSAKILGEN ENVVRIMSIH KSKGLEFPVV ILGGTGKQFN
     KMDLREDILL HETLGIGTNC IDIKKRIKYD TLQKHAIKKK CELEVLSEEM RILYVAFTRA
     KEKLIITGAV SDLEKSCENW CKASASSEDN RINPGNVLKG KSYLDWIGMA LTKHKDGDAI
     RNIGNGDITL NLDDKSNWSF KSWDRSELLE TNNNKKEKNN IDIFESNNWI ESKKDIKEVI
     EIRDRLGFKY KYIESCNTPS NISVTELKRA HQEEEFMQES YNIIDNESNE ENKKEKIKRK
     PRFMEERQEE FSAAKKGTIT HFVMQHIDLD KVTYIDEIRE EVLKMVKKEL LTEEEGKVVN
     VFKIQKFFKS DLGQRMLSSY KSGKKVYREL PFITEIPSSI IEKNLDPKIY GEEKVRLQGI
     IDAFFKEEDG YVLLDYKTDY VKEGEEENFI NKYKIQINLY KDTLNKILGE EVKEAYLYSF
     YLEKELKI
//
DBGET integrated database retrieval system