ID Q8ZVK2_PYRAE Unreviewed; 425 AA.
AC Q8ZVK2;
DT 01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2002, sequence version 1.
DT 27-MAR-2024, entry version 87.
DE RecName: Full=polynucleotide 5'-hydroxyl-kinase {ECO:0000256|ARBA:ARBA00012157};
DE EC=2.7.1.78 {ECO:0000256|ARBA:ARBA00012157};
GN OrderedLocusNames=PAE2242 {ECO:0000313|EMBL:AAL64054.1};
OS Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP
OS 104966 / NBRC 100827 / IM2).
OC Archaea; Thermoproteota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC Pyrobaculum.
OX NCBI_TaxID=178306 {ECO:0000313|EMBL:AAL64054.1, ECO:0000313|Proteomes:UP000002439};
RN [1] {ECO:0000313|EMBL:AAL64054.1, ECO:0000313|Proteomes:UP000002439}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 /
RC IM2 {ECO:0000313|Proteomes:UP000002439};
RX PubMed=11792869; DOI=10.1073/pnas.241636498;
RA Fitz-Gibbon S.T., Ladner H., Kim U.J., Stetter K.O., Simon M.I.,
RA Miller J.H.;
RT "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT aerophilum.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
CC -!- FUNCTION: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl
CC groups of both single-stranded RNA (ssRNA) and single-stranded DNA
CC (ssDNA). Exhibits a strong preference for ssRNA.
CC {ECO:0000256|ARBA:ARBA00024737}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end
CC 5'-monophospho-2'-deoxyribonucleoside-DNA + ADP + H(+);
CC Xref=Rhea:RHEA:15669, Rhea:RHEA-COMP:13180, Rhea:RHEA-COMP:13184,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:136412,
CC ChEBI:CHEBI:136416, ChEBI:CHEBI:456216; EC=2.7.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00024622};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-
CC monophospho-ribonucleoside-RNA + ADP + H(+); Xref=Rhea:RHEA:54580,
CC Rhea:RHEA-COMP:13935, Rhea:RHEA-COMP:13936, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:138282, ChEBI:CHEBI:138284,
CC ChEBI:CHEBI:456216; EC=2.7.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00024635};
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000256|ARBA:ARBA00001968};
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DR EMBL; AE009441; AAL64054.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8ZVK2; -.
DR STRING; 178306.PAE2242; -.
DR EnsemblBacteria; AAL64054; AAL64054; PAE2242.
DR KEGG; pai:PAE2242; -.
DR PATRIC; fig|178306.9.peg.1667; -.
DR eggNOG; arCOG04127; Archaea.
DR HOGENOM; CLU_051301_1_0_2; -.
DR InParanoid; Q8ZVK2; -.
DR Proteomes; UP000002439; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0051734; F:ATP-dependent polynucleotide 5'-hydroxyl-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IBA:GO_Central.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR045116; Clp1/Grc3.
DR InterPro; IPR032319; CLP1_P.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12755; CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P; 1.
DR PANTHER; PTHR12755:SF3; POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9; 1.
DR Pfam; PF16575; CLP1_P; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000002439};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 98..272
FT /note="Polyribonucleotide 5'-hydroxyl-kinase Clp1 P-loop"
FT /evidence="ECO:0000259|Pfam:PF16575"
SQ SEQUENCE 425 AA; 47684 MW; 416CF63ADD76606A CRC64;
MSIRVLKAGD VYRIEGPAKV VVRRGQVYAT GVVYTEGQSF TVLRARRLVI KALSESEVEL
VLGPGALLER VDPREEIIDE WESKTASIDP KGVIVIVGMI DVGKSTMTAM LGNKALARGY
KVAIIDADVG QNDLGPPTTV SLARLTKYIT HLRQLVAEKS IFLQATSLER IWPRAIEQIA
RAVDFAKRSW QVDTIILNTD GWVLDEEAVV FKRRLIDVIK PSLIIAIQVE KELAPVLDGY
NNVVVLPPPP QVRSRSREDR KIHREMGYGR YIFPPVELAI PLDKIPICNL PIFKGIEIGD
ELKRILARAI GTGVLKAYQV GNRVYAIIEN DAWVVRRVSG FQVIGLPIDF EKGLLVGLED
AEHFLVGLGV MKRIYYDRRK AIIYTSSEVE RRIGEVKCIR LGLVRLDDNF NEVEKAYSLL
KVEHE
//
