ID GPHA2_RAT Reviewed; 130 AA.
AC Q925Q4;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 27-MAR-2024, entry version 117.
DE RecName: Full=Glycoprotein hormone alpha-2;
DE AltName: Full=Putative secreted protein Zsig51;
DE AltName: Full=Thyrostimulin subunit alpha;
DE Flags: Precursor;
GN Name=Gpha2; Synonyms=Gpa2, Zsig51;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Dasovich M., Ching A., Lok S., Sheppard P., Webster P.;
RT "A novel cysteine knot protein expressed in pancreatic acinar cells.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as a heterodimeric glycoprotein hormone with GPHB5
CC able to bind and activate the thyroid-stimulating hormone receptor
CC (TSHR), leading to increased cAMP production. Plays a central role in
CC controlling thyroid cell metabolism. {ECO:0000250|UniProtKB:Q96T91}.
CC -!- SUBUNIT: Heterodimer with GPHB5; this heterodimer interacts with
CC thyroid-stimulating hormone receptor (TSHR), and hence stimulates cAMP
CC production. {ECO:0000250|UniProtKB:Q96T91}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycoprotein hormones subunit alpha family.
CC {ECO:0000305}.
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DR EMBL; AF260741; AAK51640.1; -; mRNA.
DR RefSeq; NP_598303.1; NM_133619.2.
DR RefSeq; XP_008758291.1; XM_008760069.2.
DR RefSeq; XP_008758292.1; XM_008760070.2.
DR AlphaFoldDB; Q925Q4; -.
DR STRING; 10116.ENSRNOP00000028536; -.
DR GlyCosmos; Q925Q4; 2 sites, No reported glycans.
DR GlyGen; Q925Q4; 2 sites.
DR PaxDb; 10116-ENSRNOP00000028536; -.
DR Ensembl; ENSRNOT00000028536.4; ENSRNOP00000028536.2; ENSRNOG00000021020.4.
DR Ensembl; ENSRNOT00055038140; ENSRNOP00055031090; ENSRNOG00055022198.
DR Ensembl; ENSRNOT00060056566; ENSRNOP00060046732; ENSRNOG00060032637.
DR Ensembl; ENSRNOT00065049205; ENSRNOP00065040351; ENSRNOG00065028539.
DR GeneID; 171158; -.
DR KEGG; rno:171158; -.
DR UCSC; RGD:621748; rat.
DR AGR; RGD:621748; -.
DR CTD; 170589; -.
DR RGD; 621748; Gpha2.
DR eggNOG; ENOG502S2RQ; Eukaryota.
DR GeneTree; ENSGT00390000009379; -.
DR HOGENOM; CLU_134026_1_0_1; -.
DR InParanoid; Q925Q4; -.
DR OMA; DRRSTCK; -.
DR OrthoDB; 5295698at2759; -.
DR PhylomeDB; Q925Q4; -.
DR TreeFam; TF332313; -.
DR Reactome; R-RNO-375281; Hormone ligand-binding receptors.
DR PRO; PR:Q925Q4; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000021020; Expressed in thymus and 7 other cell types or tissues.
DR ExpressionAtlas; Q925Q4; baseline and differential.
DR Genevisible; Q925Q4; RN.
DR GO; GO:0005576; C:extracellular region; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0046982; F:protein heterodimerization activity; ISO:RGD.
DR GO; GO:0031531; F:thyrotropin-releasing hormone receptor binding; ISO:RGD.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; ISO:RGD.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; ISO:RGD.
DR Gene3D; 2.10.90.10; Cystine-knot cytokines; 1.
DR InterPro; IPR006207; Cys_knot_C.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR000476; Glyco_hormone.
DR PANTHER; PTHR31129; GLYCOPROTEIN HORMONE ALPHA-2; 1.
DR PANTHER; PTHR31129:SF2; GLYCOPROTEIN HORMONE ALPHA-2; 1.
DR SUPFAM; SSF57501; Cystine-knot cytokines; 1.
DR PROSITE; PS01185; CTCK_1; 1.
DR PROSITE; PS01225; CTCK_2; 1.
DR PROSITE; PS50277; GLYCO_HORMONE_ALPHA_3; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Hormone; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..130
FT /note="Glycoprotein hormone alpha-2"
FT /id="PRO_0000011674"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 82
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 32..90
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 49..104
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 58..120
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 62..122
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
SQ SEQUENCE 130 AA; 14199 MW; 0BC46977131ED3DE CRC64;
MPMAPRVLLF CLLGLAVTEG HGLEAAVPIP GCHLHPFNVT VRSDRHGTCQ GSHVAQACVG
HCESSAFPSR YSVLVASGYR HNITSVSQCC TISSLKKVRV WLHCVGNQRG ELEIFTARAC
QCDMCRLSRY
//
