ID AB22G_ARATH Reviewed; 751 AA.
AC Q93YS4; Q94EX0; Q9FG17;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 24-JAN-2024, entry version 154.
DE RecName: Full=ABC transporter G family member 22;
DE Short=ABC transporter ABCG.22;
DE Short=AtABCG22;
DE AltName: Full=White-brown complex homolog protein 23;
DE Short=AtWBC23;
GN Name=ABCG22; Synonyms=WBC23; OrderedLocusNames=At5g06530; ORFNames=F15M7.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11346655; DOI=10.1074/jbc.m103104200;
RA Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.;
RT "The Arabidopsis thaliana ABC protein superfamily, a complete inventory.";
RL J. Biol. Chem. 276:30231-30244(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18299247; DOI=10.1016/j.tplants.2008.02.001;
RA Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M.,
RA Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A.,
RA Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.;
RT "Plant ABC proteins - a unified nomenclature and updated inventory.";
RL Trends Plant Sci. 13:151-159(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93YS4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93YS4-2; Sequence=VSP_020233, VSP_020234;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCG family.
CC Eye pigment precursor importer (TC 3.A.1.204) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK63861.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAN72282.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB11402.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP002543; BAB11402.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91029.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91030.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91031.1; -; Genomic_DNA.
DR EMBL; AF389289; AAK63861.1; ALT_FRAME; mRNA.
DR EMBL; AY059787; AAL24135.1; -; mRNA.
DR EMBL; AY114068; AAM45116.1; -; mRNA.
DR EMBL; BT002271; AAN72282.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001031843.1; NM_001036766.2. [Q93YS4-2]
DR RefSeq; NP_568169.1; NM_120736.3. [Q93YS4-1]
DR RefSeq; NP_850781.2; NM_180450.2. [Q93YS4-1]
DR AlphaFoldDB; Q93YS4; -.
DR SMR; Q93YS4; -.
DR BioGRID; 15820; 13.
DR IntAct; Q93YS4; 13.
DR STRING; 3702.Q93YS4; -.
DR iPTMnet; Q93YS4; -.
DR PaxDb; 3702-AT5G06530-2; -.
DR ProteomicsDB; 244370; -. [Q93YS4-1]
DR EnsemblPlants; AT5G06530.1; AT5G06530.1; AT5G06530. [Q93YS4-1]
DR EnsemblPlants; AT5G06530.2; AT5G06530.2; AT5G06530. [Q93YS4-1]
DR EnsemblPlants; AT5G06530.3; AT5G06530.3; AT5G06530. [Q93YS4-2]
DR GeneID; 830541; -.
DR Gramene; AT5G06530.1; AT5G06530.1; AT5G06530. [Q93YS4-1]
DR Gramene; AT5G06530.2; AT5G06530.2; AT5G06530. [Q93YS4-1]
DR Gramene; AT5G06530.3; AT5G06530.3; AT5G06530. [Q93YS4-2]
DR KEGG; ath:AT5G06530; -.
DR Araport; AT5G06530; -.
DR TAIR; AT5G06530; ABCG22.
DR eggNOG; KOG0061; Eukaryota.
DR InParanoid; Q93YS4; -.
DR OrthoDB; 936137at2759; -.
DR PhylomeDB; Q93YS4; -.
DR PRO; PR:Q93YS4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q93YS4; baseline and differential.
DR Genevisible; Q93YS4; AT.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR GO; GO:0010148; P:transpiration; IMP:TAIR.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013525; ABC2_TM.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR043926; ABCG_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR48041:SF94; ABC TRANSPORTER G FAMILY MEMBER 22; 1.
DR PANTHER; PTHR48041; ABC TRANSPORTER G FAMILY MEMBER 28; 1.
DR Pfam; PF01061; ABC2_membrane; 1.
DR Pfam; PF19055; ABC2_membrane_7; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Membrane; Nucleotide-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..751
FT /note="ABC transporter G family member 22"
FT /id="PRO_0000240694"
FT TRANSMEM 516..536
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 552..572
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 602..622
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 634..654
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 666..686
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 722..742
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 157..403
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 498..707
FT /note="ABC transmembrane type-2"
FT REGION 26..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 197..204
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT VAR_SEQ 681..691
FT /note="KVPVFISWIRY -> ASPLFLDFLCF (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_020233"
FT VAR_SEQ 692..751
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_020234"
FT CONFLICT 131
FT /note="D -> G (in Ref. 3; AAK63861/AAN72282)"
FT /evidence="ECO:0000305"
FT CONFLICT 536
FT /note="W -> R (in Ref. 3; AAK63861/AAN72282)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 751 AA; 82931 MW; 5564454F99D0A2EC CRC64;
MSMEKPPLAS GLARTRSEQL YETVAADIRS PHGSMDANGV PATAPAAVGG GGTLSRKSSR
RLMGMSPGRS SGAGTHIRKS RSAQLKLELE EVSSGAALSR ASSASLGLSF SFTGFAMPPE
EISDSKPFSD DEMIPEDIEA GKKKPKFQAE PTLPIFLKFR DVTYKVVIKK LTSSVEKEIL
TGISGSVNPG EVLALMGPSG SGKTTLLSLL AGRISQSSTG GSVTYNDKPY SKYLKSKIGF
VTQDDVLFPH LTVKETLTYA ARLRLPKTLT REQKKQRALD VIQELGLERC QDTMIGGAFV
RGVSGGERKR VSIGNEIIIN PSLLLLDEPT SGLDSTTALR TILMLHDIAE AGKTVITTIH
QPSSRLFHRF DKLILLGRGS LLYFGKSSEA LDYFSSIGCS PLIAMNPAEF LLDLANGNIN
DISVPSELDD RVQVGNSGRE TQTGKPSPAA VHEYLVEAYE TRVAEQEKKK LLDPVPLDEE
AKAKSTRLKR QWGTCWWEQY CILFCRGLKE RRHEYFSWLR VTQVLSTAVI LGLLWWQSDI
RTPMGLQDQA GLLFFIAVFW GFFPVFTAIF AFPQERAMLN KERAADMYRL SAYFLARTTS
DLPLDFILPS LFLLVVYFMT GLRISPYPFF LSMLTVFLCI IAAQGLGLAI GAILMDLKKA
TTLASVTVMT FMLAGGFFVK KVPVFISWIR YLSFNYHTYK LLLKVQYQDF AVSINGMRID
NGLTEVAALV VMIFGYRLLA YLSLRQMKIV T
//
