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Database: UniProt
Entry: Q94C75
LinkDB: Q94C75
Original site: Q94C75 
ID   RH25_ARATH              Reviewed;         563 AA.
AC   Q94C75; Q9ZS00;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 2.
DT   16-OCT-2019, entry version 114.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 25;
DE            EC=3.6.4.13;
GN   Name=RH25; OrderedLocusNames=At5g08620; ORFNames=MAH20.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9862990; DOI=10.1093/nar/27.2.628;
RA   Aubourg S., Kreis M., Lecharny A.;
RT   "The DEAD box RNA helicase family in Arabidopsis thaliana.";
RL   Nucleic Acids Res. 27:628-636(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA   Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. II.
RT   Sequence features of the regions of 1,044,062 bp covered by thirteen
RT   physically assigned P1 clones.";
RL   DNA Res. 4:291-300(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana
RT   reference genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17168887; DOI=10.1111/j.1467-7652.2004.00084.x;
RA   Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M.,
RA   Lecharny A.;
RT   "DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link
RT   between quantitative expression, gene structure and evolution of a
RT   family of genes.";
RL   Plant Biotechnol. J. 2:401-415(2004).
RN   [6]
RP   GENE FAMILY.
RX   PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA   Xu R., Zhang S., Huang J., Zheng C.;
RT   "Genome-wide comparative in silico analysis of the RNA helicase gene
RT   family in Zea mays and Glycine max: a comparison with Arabidopsis and
RT   Oryza sativa.";
RL   PLoS ONE 8:E78982-E78982(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family.
CC       {ECO:0000305}.
DR   EMBL; AJ010473; CAA09212.1; -; mRNA.
DR   EMBL; AB006697; BAB10011.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED91330.1; -; Genomic_DNA.
DR   EMBL; AY035114; AAK59619.1; -; mRNA.
DR   EMBL; AY142638; AAN13096.1; -; mRNA.
DR   PIR; T51348; T51348.
DR   RefSeq; NP_196479.1; NM_120949.4.
DR   SMR; Q94C75; -.
DR   BioGrid; 16041; 1.
DR   STRING; 3702.AT5G08620.1; -.
DR   PaxDb; Q94C75; -.
DR   PRIDE; Q94C75; -.
DR   EnsemblPlants; AT5G08620.1; AT5G08620.1; AT5G08620.
DR   GeneID; 830763; -.
DR   Gramene; AT5G08620.1; AT5G08620.1; AT5G08620.
DR   KEGG; ath:AT5G08620; -.
DR   Araport; AT5G08620; -.
DR   TAIR; locus:2159527; AT5G08620.
DR   eggNOG; KOG0342; Eukaryota.
DR   eggNOG; COG0513; LUCA.
DR   HOGENOM; HOG000268799; -.
DR   InParanoid; Q94C75; -.
DR   OMA; KAIVFAP; -.
DR   OrthoDB; 537587at2759; -.
DR   PhylomeDB; Q94C75; -.
DR   PRO; PR:Q94C75; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q94C75; baseline and differential.
DR   Genevisible; Q94C75; AT.
DR   GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IDA:TAIR.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009409; P:response to cold; IEP:TAIR.
DR   GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR   GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome; RNA-binding.
FT   CHAIN         1    563       DEAD-box ATP-dependent RNA helicase 25.
FT                                /FTId=PRO_0000239165.
FT   DOMAIN      111    294       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      328    479       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     124    131       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF        80    108       Q motif.
FT   MOTIF       242    245       DEAD box.
FT   MOD_RES      25     25       Phosphoserine.
FT                                {ECO:0000250|UniProtKB:Q9FNM7}.
FT   CONFLICT    119    119       D -> N (in Ref. 4; AAK59619).
FT                                {ECO:0000305}.
SQ   SEQUENCE   563 AA;  62495 MW;  A0E4BD8BF745A41E CRC64;
     MNSDGPKSGK KRREIRAKLV KKLTSDEDGS GKLVKDNNKS LKRGREGKSD VDEPLIKKPA
     STTPLVTQIA KTSDSYLSKT RFDQFPLSPL TLKGIEDAGF KTMTVVQEAT LPLILQGKDI
     LAKAKTGTGK TVAFLLPSIE AVIKAPPASR DNRHPPIIVL VVCPTRELAC QAAAEANILL
     KYHPSIGVQV VIGGTKLPTE QRRLQKSPCQ ILVATPGRLK DHIDNTSGFA TRLMGVKVLV
     LDEADHLLDM GFRREIERII AAVPKQRQTF LFSATVSDEV RQICHVALKR DHEFVNCVQE
     GAGETHQKVS QMYMIASLDR HFSLLYGLLK KHITDNVGYK VIIFCTTAMV TRLVADLLGK
     LSLNVREIHS RKPQSYRTRV SDEFRKSKSI ILVTSDVSAR GVDYPDVSLV VQMGLPSDRE
     QYIHRLGRTG RKGKEGEGVL LLAPWEEYFL SSVKDLPITK SSLPPIDHEA VKKVQKGLIQ
     VEMTNKEAAY QAWLGYYKSQ KKIARDTTRL VELANEFSRS MGLSIPPAIP VNILGKMGLK
     NVPGIRVAPG FDKKPAKRNY RSR
//
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