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Database: UniProt
Entry: Q95YF3
LinkDB: Q95YF3
Original site: Q95YF3 
ID   CGH1_CAEEL              Reviewed;         430 AA.
AC   Q95YF3;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   16-OCT-2019, entry version 149.
DE   RecName: Full=ATP-dependent RNA helicase cgh-1;
DE            EC=3.6.4.13;
DE   AltName: Full=Conserved germline helicase 1;
GN   Name=cgh-1; ORFNames=C07H6.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   PROTEIN SEQUENCE OF 21-28; 34-61; 111-121; 158-178; 188-215 AND
RP   333-342, AND IDENTIFICATION BY MASS SPECTROMETRY.
RA   Bienvenut W.V.;
RL   Submitted (MAR-2006) to UniProtKB.
RN   [3] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=11546739;
RA   Navarro R.E., Shim E.Y., Kohara Y., Singson A., Blackwell T.K.;
RT   "cgh-1, a conserved predicted RNA helicase required for gametogenesis
RT   and protection from physiological germline apoptosis in C. elegans.";
RL   Development 128:3221-3232(2001).
RN   [4] {ECO:0000305}
RP   FUNCTION, AND INTERACTION WITH CAR-1.
RX   PubMed=16221731; DOI=10.1242/dev.02060;
RA   Boag P.R., Nakamura A., Blackwell T.K.;
RT   "A conserved RNA-protein complex component involved in physiological
RT   germline apoptosis regulation in C. elegans.";
RL   Development 132:4975-4986(2005).
RN   [5]
RP   INTERACTION WITH IFET-1, AND SUBCELLULAR LOCATION.
RX   PubMed=23264733; DOI=10.1242/jcs.119834;
RA   Sengupta M.S., Low W.Y., Patterson J.R., Kim H.M., Traven A.,
RA   Beilharz T.H., Colaiacovo M.P., Schisa J.A., Boag P.R.;
RT   "ifet-1 is a broad-scale translational repressor required for normal P
RT   granule formation in C. elegans.";
RL   J. Cell Sci. 126:850-859(2013).
RN   [6]
RP   INTERACTION WITH OMA-1.
RX   PubMed=25261697; DOI=10.1534/genetics.114.168823;
RA   Spike C.A., Coetzee D., Nishi Y., Guven-Ozkan T., Oldenbroek M.,
RA   Yamamoto I., Lin R., Greenstein D.;
RT   "Translational control of the oogenic program by components of OMA
RT   ribonucleoprotein particles in Caenorhabditis elegans.";
RL   Genetics 198:1513-1533(2014).
CC   -!- FUNCTION: Probable RNA helicase required for oocyte and sperm
CC       function. Also required to prevent the physiological germline
CC       apoptosis mechanism killing essentially all developing oocytes.
CC       {ECO:0000269|PubMed:11546739, ECO:0000269|PubMed:16221731}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Interacts with car-1 in a germline ribonucleoprotein
CC       complex (PubMed:16221731). Interacts with ifet-1
CC       (PubMed:23264733). Interacts with oma-1, which is a component of a
CC       ribonucleoprotein complex, in an RNA-dependent manner
CC       (PubMed:25261697). {ECO:0000269|PubMed:16221731,
CC       ECO:0000269|PubMed:23264733, ECO:0000269|PubMed:25261697}.
CC   -!- INTERACTION:
CC       Q21740:edc-3; NbExp=5; IntAct=EBI-314525, EBI-314602;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11546739}.
CC       Note=Cytoplasmic when associated with pgl-1. Associates with P
CC       granules and this localization is dependent on ifet-1.
CC       {ECO:0000269|PubMed:11546739, ECO:0000269|PubMed:23264733}.
CC   -!- TISSUE SPECIFICITY: Expression is restricted to two germline
CC       precursors Z2 and Z3 in L1 stage hermaphrodites, and is detectable
CC       specifically in the gonad at low levels into the L3 stage.
CC       Expression is significantly higher during the early L4 stage. In
CC       adults, expression remains gonad-specific and was not apparent in
CC       the somatically derived uterus. Expressed in germ granules (P
CC       granules); when associated with pgl-1.
CC       {ECO:0000269|PubMed:11546739}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX6/DHH1
CC       subfamily. {ECO:0000255}.
DR   EMBL; FO080423; CCD63592.1; -; Genomic_DNA.
DR   RefSeq; NP_498646.1; NM_066245.5.
DR   SMR; Q95YF3; -.
DR   BioGrid; 41269; 32.
DR   DIP; DIP-25457N; -.
DR   IntAct; Q95YF3; 23.
DR   STRING; 6239.C07H6.5; -.
DR   iPTMnet; Q95YF3; -.
DR   EPD; Q95YF3; -.
DR   PaxDb; Q95YF3; -.
DR   PeptideAtlas; Q95YF3; -.
DR   PRIDE; Q95YF3; -.
DR   EnsemblMetazoa; C07H6.5.1; C07H6.5.1; WBGene00000479.
DR   GeneID; 176061; -.
DR   KEGG; cel:CELE_C07H6.5; -.
DR   UCSC; C07H6.5.1; c. elegans.
DR   CTD; 176061; -.
DR   WormBase; C07H6.5; CE00839; WBGene00000479; cgh-1.
DR   eggNOG; KOG0326; Eukaryota.
DR   eggNOG; ENOG410XRAZ; LUCA.
DR   GeneTree; ENSGT00940000170366; -.
DR   HOGENOM; HOG000268797; -.
DR   InParanoid; Q95YF3; -.
DR   KO; K12614; -.
DR   OMA; DWNLMSS; -.
DR   OrthoDB; 583315at2759; -.
DR   PhylomeDB; Q95YF3; -.
DR   Reactome; R-CEL-430039; mRNA decay by 5' to 3' exoribonuclease.
DR   PRO; PR:Q95YF3; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00000479; Expressed in 8 organ(s), highest expression level in adult organism.
DR   GO; GO:0010494; C:cytoplasmic stress granule; IDA:WormBase.
DR   GO; GO:0043229; C:intracellular organelle; IDA:WormBase.
DR   GO; GO:1990124; C:messenger ribonucleoprotein complex; IDA:WormBase.
DR   GO; GO:0043186; C:P granule; IDA:WormBase.
DR   GO; GO:0000932; C:P-body; IDA:WormBase.
DR   GO; GO:1990904; C:ribonucleoprotein complex; IDA:WormBase.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; ISS:WormBase.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0033962; P:cytoplasmic mRNA processing body assembly; IMP:WormBase.
DR   GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR   GO; GO:0007276; P:gamete generation; IMP:WormBase.
DR   GO; GO:0016071; P:mRNA metabolic process; IMP:WormBase.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; IMP:WormBase.
DR   GO; GO:0017148; P:negative regulation of translation; IMP:WormBase.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0034063; P:stress granule assembly; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   1: Evidence at protein level;
KW   Apoptosis; ATP-binding; Complete proteome; Cytoplasm;
KW   Developmental protein; Differentiation; Direct protein sequencing;
KW   Helicase; Hydrolase; Nucleotide-binding; Oogenesis;
KW   Reference proteome; RNA-binding; Spermatogenesis.
FT   CHAIN         1    430       ATP-dependent RNA helicase cgh-1.
FT                                /FTId=PRO_0000239936.
FT   DOMAIN       74    244       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      254    414       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      87     94       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF        43     71       Q motif. {ECO:0000255}.
FT   MOTIF       192    195       DEAD box. {ECO:0000255}.
SQ   SEQUENCE   430 AA;  48713 MW;  0C31C5597DD8B5D4 CRC64;
     MSGAEQQQIV PANNGDENWK AGLNLPAKDR RFKTADVTDT KGVEFEDFCL GRDLLMGIFE
     KGWEKPSPIQ EASIGVALTG QDILARAKNG TGKTGAYCIP VIEKIQPALK AIQAMVIVPT
     RELALQTSQI CVELSKHIQL KVMVTTGGTD LRDDIMRLNG TVHLVIATPG RILDLMEKGV
     AKMEHCKTLV LDEADKLLSQ DFQGILDRLI NFLPKERQVM LYSATFPNTV TSFMQKHMHK
     PYEINLMEEL TLLGVTQYYA FVQEKQKVHC LNTLFRKLQI NQSIIFCNST QRVELLAKKI
     TEIGYSCYYI HSKMAQNHRN RVFHDFRQGN CRNLVCSDLL TRGIDIQAVN VVINFDFPRN
     AETYLHRIGR SGRFGHLGVA INLITYEDRH TLRRIEQELR TRIEPIPKTV DPKLYVADQQ
     LVDAADETTA
//
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