GenomeNet

Database: UniProt
Entry: Q96DE5
LinkDB: Q96DE5
Original site: Q96DE5 
ID   APC16_HUMAN             Reviewed;         110 AA.
AC   Q96DE5;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   17-JUN-2020, entry version 134.
DE   RecName: Full=Anaphase-promoting complex subunit 16;
DE            Short=APC16;
DE   AltName: Full=Cyclosome subunit 16;
GN   Name=ANAPC16; Synonyms=C10orf104, CENP-27 {ECO:0000303|PubMed:20813266};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15164054; DOI=10.1038/nature02462;
RA   Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA   Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA   Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA   Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA   Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA   Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA   Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA   Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA   Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA   Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA   Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA   Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA   Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA   Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA   McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA   Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA   Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA   Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA   Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA   Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA   Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA   Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA   Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT   "The DNA sequence and comparative analysis of human chromosome 10.";
RL   Nature 429:375-381(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=20813266; DOI=10.1016/j.cell.2010.07.047;
RA   Ohta S., Bukowski-Wills J.C., Sanchez-Pulido L., Alves Fde L., Wood L.,
RA   Chen Z.A., Platani M., Fischer L., Hudson D.F., Ponting C.P., Fukagawa T.,
RA   Earnshaw W.C., Rappsilber J.;
RT   "The protein composition of mitotic chromosomes determined using
RT   multiclassifier combinatorial proteomics.";
RL   Cell 142:810-821(2010).
RN   [4]
RP   FUNCTION, SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=20360068; DOI=10.1126/science.1181348;
RA   Hutchins J.R., Toyoda Y., Hegemann B., Poser I., Heriche J.K., Sykora M.M.,
RA   Augsburg M., Hudecz O., Buschhorn B.A., Bulkescher J., Conrad C.,
RA   Comartin D., Schleiffer A., Sarov M., Pozniakovsky A., Slabicki M.M.,
RA   Schloissnig S., Steinmacher I., Leuschner M., Ssykor A., Lawo S.,
RA   Pelletier L., Stark H., Nasmyth K., Ellenberg J., Durbin R., Buchholz F.,
RA   Mechtler K., Hyman A.A., Peters J.M.;
RT   "Systematic analysis of human protein complexes identifies chromosome
RT   segregation proteins.";
RL   Science 328:593-599(2010).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [6]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP   METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN   [7]
RP   STRUCTURE BY ELECTRON MICROSCOPY (7.4 ANGSTROMS) OF THE APC/C, AND SUBUNIT.
RX   PubMed=25043029; DOI=10.1038/nature13543;
RA   Chang L., Zhang Z., Yang J., McLaughlin S.H., Barford D.;
RT   "Molecular architecture and mechanism of the anaphase-promoting complex.";
RL   Nature 513:388-393(2014).
RN   [8] {ECO:0000244|PDB:4UI9, ECO:0000244|PDB:5A31}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.60 ANGSTROMS) OF APC/C, AND SUBUNIT.
RX   PubMed=26083744; DOI=10.1038/nature14471;
RA   Chang L., Zhang Z., Yang J., McLaughlin S.H., Barford D.;
RT   "Atomic structure of the APC/C and its mechanism of protein
RT   ubiquitination.";
RL   Nature 522:450-454(2015).
CC   -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC       (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls
CC       progression through mitosis and the G1 phase of the cell cycle. The
CC       APC/C complex acts by mediating ubiquitination and subsequent
CC       degradation of target proteins: it mainly mediates the formation of
CC       'Lys-11'-linked polyubiquitin chains and, to a lower extent, the
CC       formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
CC       {ECO:0000269|PubMed:20360068}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: The mammalian APC/C is composed at least of 14 distinct
CC       subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6,
CC       ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and
CC       ANAPC16 that assemble into a complex of at least 19 chains with a
CC       combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1
CC       and FBXO5 (PubMed:26083744, PubMed:25043029). ANAPC16 associates with
CC       the rest of the complex independently of ANAPC2 and ANAPC11.
CC       {ECO:0000269|PubMed:20360068, ECO:0000269|PubMed:25043029,
CC       ECO:0000269|PubMed:26083744}.
CC   -!- INTERACTION:
CC       Q96DE5; O75031: HSF2BP; NbExp=3; IntAct=EBI-2555937, EBI-7116203;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20360068}. Nucleus
CC       {ECO:0000269|PubMed:20360068}. Chromosome, centromere, kinetochore
CC       {ECO:0000269|PubMed:20813266}.
CC   -!- SIMILARITY: Belongs to the APC16 family. {ECO:0000305}.
DR   EMBL; AL607035; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC009530; AAH09530.1; -; mRNA.
DR   CCDS; CCDS7314.1; -.
DR   RefSeq; NP_001229475.1; NM_001242546.1.
DR   RefSeq; NP_001229476.1; NM_001242547.1.
DR   RefSeq; NP_775744.1; NM_173473.3.
DR   PDB; 4RG6; X-ray; 3.30 A; S=74-109.
DR   PDB; 4RG9; X-ray; 3.25 A; S=74-109.
DR   PDB; 4UI9; EM; 3.60 A; E=1-110.
DR   PDB; 5A31; EM; 4.30 A; E=1-110.
DR   PDB; 5G04; EM; 4.00 A; E=1-110.
DR   PDB; 5G05; EM; 3.40 A; E=1-110.
DR   PDB; 5KHR; EM; 6.10 A; E=1-110.
DR   PDB; 5KHU; EM; 4.80 A; E=1-110.
DR   PDB; 5L9T; EM; 6.40 A; E=1-110.
DR   PDB; 5L9U; EM; 6.40 A; E=1-110.
DR   PDB; 5LCW; EM; 4.00 A; E=1-110.
DR   PDB; 6Q6G; EM; 3.20 A; H=1-110.
DR   PDB; 6Q6H; EM; 3.20 A; H=1-110.
DR   PDB; 6TLJ; EM; 3.80 A; E=1-110.
DR   PDB; 6TM5; EM; 3.90 A; E=1-110.
DR   PDBsum; 4RG6; -.
DR   PDBsum; 4RG9; -.
DR   PDBsum; 4UI9; -.
DR   PDBsum; 5A31; -.
DR   PDBsum; 5G04; -.
DR   PDBsum; 5G05; -.
DR   PDBsum; 5KHR; -.
DR   PDBsum; 5KHU; -.
DR   PDBsum; 5L9T; -.
DR   PDBsum; 5L9U; -.
DR   PDBsum; 5LCW; -.
DR   PDBsum; 6Q6G; -.
DR   PDBsum; 6Q6H; -.
DR   PDBsum; 6TLJ; -.
DR   PDBsum; 6TM5; -.
DR   SMR; Q96DE5; -.
DR   BioGRID; 125643; 41.
DR   DIP; DIP-56449N; -.
DR   IntAct; Q96DE5; 34.
DR   MINT; Q96DE5; -.
DR   STRING; 9606.ENSP00000477760; -.
DR   iPTMnet; Q96DE5; -.
DR   MetOSite; Q96DE5; -.
DR   PhosphoSitePlus; Q96DE5; -.
DR   BioMuta; ANAPC16; -.
DR   EPD; Q96DE5; -.
DR   jPOST; Q96DE5; -.
DR   MassIVE; Q96DE5; -.
DR   MaxQB; Q96DE5; -.
DR   PaxDb; Q96DE5; -.
DR   PeptideAtlas; Q96DE5; -.
DR   PRIDE; Q96DE5; -.
DR   ProteomicsDB; 76284; -.
DR   Antibodypedia; 48879; 13 antibodies.
DR   Ensembl; ENST00000299381; ENSP00000299381; ENSG00000166295.
DR   Ensembl; ENST00000621663; ENSP00000477760; ENSG00000166295.
DR   GeneID; 119504; -.
DR   KEGG; hsa:119504; -.
DR   UCSC; uc001jsv.4; human.
DR   CTD; 119504; -.
DR   EuPathDB; HostDB:ENSG00000166295.8; -.
DR   GeneCards; ANAPC16; -.
DR   HGNC; HGNC:26976; ANAPC16.
DR   HPA; ENSG00000166295; Low tissue specificity.
DR   MIM; 613427; gene.
DR   neXtProt; NX_Q96DE5; -.
DR   OpenTargets; ENSG00000166295; -.
DR   PharmGKB; PA165548225; -.
DR   eggNOG; ENOG410IX3S; Eukaryota.
DR   eggNOG; ENOG4111QQQ; LUCA.
DR   GeneTree; ENSGT00390000018109; -.
DR   HOGENOM; CLU_153312_0_0_1; -.
DR   InParanoid; Q96DE5; -.
DR   OMA; XGSERFL; -.
DR   OrthoDB; 1642213at2759; -.
DR   PhylomeDB; Q96DE5; -.
DR   TreeFam; TF332754; -.
DR   Reactome; R-HSA-141430; Inactivation of APC/C via direct inhibition of the APC/C complex.
DR   Reactome; R-HSA-174048; APC/C:Cdc20 mediated degradation of Cyclin B.
DR   Reactome; R-HSA-174084; Autodegradation of Cdh1 by Cdh1:APC/C.
DR   Reactome; R-HSA-174154; APC/C:Cdc20 mediated degradation of Securin.
DR   Reactome; R-HSA-174178; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
DR   Reactome; R-HSA-174184; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
DR   Reactome; R-HSA-176407; Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
DR   Reactome; R-HSA-176408; Regulation of APC/C activators between G1/S and early anaphase.
DR   Reactome; R-HSA-176409; APC/C:Cdc20 mediated degradation of mitotic proteins.
DR   Reactome; R-HSA-176412; Phosphorylation of the APC/C.
DR   Reactome; R-HSA-179409; APC-Cdc20 mediated degradation of Nek2A.
DR   Reactome; R-HSA-2467813; Separation of Sister Chromatids.
DR   Reactome; R-HSA-2559582; Senescence-Associated Secretory Phenotype (SASP).
DR   Reactome; R-HSA-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR   Reactome; R-HSA-8853884; Transcriptional Regulation by VENTX.
DR   UniPathway; UPA00143; -.
DR   BioGRID-ORCS; 119504; 3 hits in 788 CRISPR screens.
DR   ChiTaRS; ANAPC16; human.
DR   GenomeRNAi; 119504; -.
DR   Pharos; Q96DE5; Tdark.
DR   PRO; PR:Q96DE5; -.
DR   Proteomes; UP000005640; Chromosome 10.
DR   RNAct; Q96DE5; protein.
DR   Bgee; ENSG00000166295; Expressed in parotid gland and 206 other tissues.
DR   ExpressionAtlas; Q96DE5; baseline and differential.
DR   Genevisible; Q96DE5; HS.
DR   GO; GO:0005680; C:anaphase-promoting complex; IDA:UniProtKB.
DR   GO; GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; IDA:HPA.
DR   GO; GO:0000776; C:kinetochore; IDA:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; TAS:Reactome.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
DR   GO; GO:1901990; P:regulation of mitotic cell cycle phase transition; TAS:Reactome.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:Reactome.
DR   DisProt; DP01455; -.
DR   InterPro; IPR029641; APC16.
DR   PANTHER; PTHR31564; PTHR31564; 1.
DR   Pfam; PF17256; ANAPC16; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Cell cycle; Cell division; Centromere;
KW   Chromosome; Cytoplasm; Kinetochore; Mitosis; Nucleus; Reference proteome;
KW   Ubl conjugation pathway.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000244|PubMed:22814378"
FT   CHAIN           2..110
FT                   /note="Anaphase-promoting complex subunit 16"
FT                   /id="PRO_0000089816"
FT   COMPBIAS        4..26
FT                   /note="Ser-rich"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000244|PubMed:22814378"
FT   HELIX           53..90
FT                   /evidence="ECO:0000244|PDB:6Q6G"
FT   HELIX           91..95
FT                   /evidence="ECO:0000244|PDB:6Q6G"
FT   HELIX           99..102
FT                   /evidence="ECO:0000244|PDB:6Q6G"
SQ   SEQUENCE   110 AA;  11667 MW;  F348FA92F4B89E4F CRC64;
     MAASSSSSSA GGVSGSSVTG SGFSVSDLAP PRKALFTYPK GAGEMLEDGS ERFLCESVFS
     YQVASTLKQV KHDQQVARME KLAGLVEELE ADEWRFKPIE QLLGFTPSSG
//
DBGET integrated database retrieval system