ID Q970I2_SULTO Unreviewed; 439 AA.
AC Q970I2;
DT 01-DEC-2001, integrated into UniProtKB/TrEMBL.
DT 01-DEC-2001, sequence version 1.
DT 27-MAR-2024, entry version 129.
DE SubName: Full=DNA-dependent ATPase XPBII {ECO:0000313|EMBL:BAB66691.1};
GN Name=xpb2 {ECO:0000313|EMBL:BAB66691.1};
GN Synonyms=ST1613 {ECO:0000313|EMBL:BAB66691.1};
GN OrderedLocusNames=STK_16130 {ECO:0000313|EMBL:BAB66691.1};
OS Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
OS (Sulfolobus tokodaii).
OC Archaea; Thermoproteota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfurisphaera.
OX NCBI_TaxID=273063 {ECO:0000313|EMBL:BAB66691.1, ECO:0000313|Proteomes:UP000001015};
RN [1] {ECO:0000313|Proteomes:UP000001015}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7
RC {ECO:0000313|Proteomes:UP000001015};
RX PubMed=11572479; DOI=10.1093/dnares/8.4.123;
RA Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M.,
RA Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y.,
RA Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T.,
RA Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K.,
RA Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.;
RT "Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon,
RT Sulfolobus tokodaii strain7.";
RL DNA Res. 8:123-140(2001).
RN [2] {ECO:0007829|PDB:5TNU}
RP X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS).
RX PubMed=29285929; DOI=10.1021/acs.analchem.7b04515;
RA Kahanda D., DuPrez K.T., Hilario E., McWilliams M.A., Wohlgamuth C.H.,
RA Fan L., Slinker J.D.;
RT "Application of Electrochemical Devices to Characterize the Dynamic Actions
RT of Helicases on DNA.";
RL Anal. Chem. 90:2178-2185(2018).
RN [3] {ECO:0007829|PDB:6P4O}
RP X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS).
RA Fan L., DuPrez K.T.;
RT "Complex of XPB helicase with Bax1 endonuclease from Sulfurisphaera
RT tokodaii at 3.15 Angstroms resolution.";
RL Submitted (MAY-2019) to the PDB data bank.
RN [4] {ECO:0007829|PDB:6P4W}
RP X-RAY CRYSTALLOGRAPHY (2.96 ANGSTROMS) OF 2-439.
RA Fan L., He F., DuPrez K.T.;
RT "XPB helicase in a complex with truncated Bax1 from Sulfurisphaera tokodaii
RT at 2.96 Angstrom resolution.";
RL Submitted (MAY-2019) to the PDB data bank.
RN [5] {ECO:0007829|PDB:6P4F}
RP X-RAY CRYSTALLOGRAPHY (3.55 ANGSTROMS) IN COMPLEX WITH MG(2+).
RX PubMed=32986831; DOI=10.1093/nar/gkaa801;
RA He F., DuPrez K., Hilario E., Chen Z., Fan L.;
RT "Structural basis of the XPB helicase-Bax1 nuclease complex interacting
RT with the repair bubble DNA.";
RL Nucleic Acids Res. 48:11695-11705(2020).
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000023; BAB66691.1; -; Genomic_DNA.
DR RefSeq; WP_010979669.1; NC_003106.2.
DR PDB; 5TNU; X-ray; 3.05 A; A/B=1-439.
DR PDB; 6P4F; X-ray; 3.55 A; A/C=1-439.
DR PDB; 6P4O; X-ray; 3.15 A; A/C/E=1-439.
DR PDB; 6P4W; X-ray; 2.96 A; A/C=2-439.
DR PDBsum; 5TNU; -.
DR AlphaFoldDB; Q970I2; -.
DR SMR; Q970I2; -.
DR STRING; 273063.STK_16130; -.
DR GeneID; 1459655; -.
DR KEGG; sto:STK_16130; -.
DR PATRIC; fig|273063.9.peg.1839; -.
DR eggNOG; arCOG00874; Archaea.
DR OrthoDB; 11644at2157; -.
DR Proteomes; UP000001015; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR CDD; cd17926; DEXHc_RE; 1.
DR CDD; cd18789; SF2_C_XPB; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR040699; XPB_DRD.
DR PANTHER; PTHR11274:SF0; GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB; 1.
DR PANTHER; PTHR11274; RAD25/XP-B DNA REPAIR HELICASE; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF18458; XPB_DRD; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:5TNU, ECO:0007829|PDB:6P4F};
KW Metal-binding {ECO:0007829|PDB:6P4F, ECO:0007829|PDB:6P4W};
KW Reference proteome {ECO:0000313|Proteomes:UP000001015}.
FT DOMAIN 77..221
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 311..439
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT BINDING 277
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:6P4F"
FT BINDING 403
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:6P4F"
SQ SEQUENCE 439 AA; 50634 MW; 0B1F61D4A9D58177 CRC64;
MVYLRYFKGL ILSDAYAPGL KWSDELKAYS ALAFKYRDVR KYFLEKEIEV EENVIDSLPF
PLIKDKIELR DYQAEAVKAW LKEKRGIIVL PTGAGKTQVA LKIVSIMKVA TLIVVPTIDL
ITQWKERINK YLDFDPGIIG GGEDSLKGIT VITYDSAYTR AEELGNKFPL LIFDEVHHLP
SEGYSIMAQL FASPYRLGLT ATPERDDGKH ELYPILVGPI VYRKSVEELA GKYIAKYKIK
KLYVSLTNEE KKRYDGLRKK LKDFLSSRGL KLQNLDDFHR LVKLAAKDKE AREALLAWHE
SLNIAVNSQS KIEKLREILQ EYKNEKIIVF TRDTQMAYRI SKTFLIPVVT YKTDKDEREE
ILQKFRDGEY RVIVASTVFD EGVDVPDATL AIVMGGYGTK RQFLQRLGRI LRKKDKEALL
IEIVTKGTAD YRLSRRRRE
//
