ID Q97DE4_CLOAB Unreviewed; 571 AA.
AC Q97DE4;
DT 01-OCT-2001, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2001, sequence version 1.
DT 27-MAR-2024, entry version 103.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=CA_C3534 {ECO:0000313|EMBL:AAK81459.1};
OS Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS / VKM B-1787).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=272562 {ECO:0000313|EMBL:AAK81459.1, ECO:0000313|Proteomes:UP000000814};
RN [1] {ECO:0000313|EMBL:AAK81459.1, ECO:0000313|Proteomes:UP000000814}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787
RC {ECO:0000313|Proteomes:UP000000814};
RX PubMed=11466286; DOI=10.1128/JB.183.16.4823-4838.2001;
RA Nolling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA Doucette-Stamm L., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA Smith D.R.;
RT "Genome sequence and comparative analysis of the solvent-producing
RT bacterium Clostridium acetobutylicum.";
RL J. Bacteriol. 183:4823-4838(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE001437; AAK81459.1; -; Genomic_DNA.
DR PIR; H97333; H97333.
DR RefSeq; NP_350119.1; NC_003030.1.
DR RefSeq; WP_010966799.1; NC_003030.1.
DR AlphaFoldDB; Q97DE4; -.
DR STRING; 272562.CA_C3534; -.
DR REBASE; 5525; M.Cac824ORF3534P.
DR GeneID; 45000025; -.
DR KEGG; cac:CA_C3534; -.
DR PATRIC; fig|272562.8.peg.3723; -.
DR eggNOG; COG0827; Bacteria.
DR HOGENOM; CLU_020255_1_0_9; -.
DR OrthoDB; 9815272at2; -.
DR Proteomes; UP000000814; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000000814};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..266
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 571 AA; 67333 MW; 3888190497D73135 CRC64;
MLLRKDATEE RLTGRYFTPK DLASYIIDWV IQDNNINKIL EPSCGNGVFL ECLGERRLED
GRNITAIEID EDVSFEASMQ IDNSLRFNNC YDYQRALRND GDIVNNGIVI INDDFYKVYE
QELQGQRFQA IVGNPPYIRY QYLSEQQREE QSKILIRNNM RPNKLINAWV SFVVACAEIL
DGNGKMGLVI PAELLQVAYA EDLRRFIMRT FQRITIVTFR ELVFPNVQQE VVLLLVEKEI
LHTREHQLRI VEYQDINELT ESNDLDEYPF NDVEINESKW TKYFLSANDI RLINNIREND
KFVRFSDVAR VEVGITTGNN DYFCVNRRVV EEYDLERVCR PLIARSVNIN GVKFTYEDWK
SNIENGAKTY LIDFPDVPYD EYQLSYKQYI EYGIHNDKNS GYKCRIRDRW YKVPSIWVPD
AFFLRRNHLY PKFVLNSQEV NAVSTDTMHR VRFNNHDDRE RILLSYYNSI ALAFTEIEGR
SYGGGVLEIL PREVGRIIMP DLRNRELIDD ATVSNLLNRI DGYIRSNDNI LGIVDEIDEI
ILVEIMGVDE NVVREFRNIW LTLQRRRLNR R
//
