ID Q97HA2_CLOAB Unreviewed; 570 AA.
AC Q97HA2;
DT 01-OCT-2001, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2001, sequence version 1.
DT 27-MAR-2024, entry version 91.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN OrderedLocusNames=CA_C2111 {ECO:0000313|EMBL:AAK80069.1};
OS Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS / VKM B-1787).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=272562 {ECO:0000313|EMBL:AAK80069.1, ECO:0000313|Proteomes:UP000000814};
RN [1] {ECO:0000313|EMBL:AAK80069.1, ECO:0000313|Proteomes:UP000000814}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787
RC {ECO:0000313|Proteomes:UP000000814};
RX PubMed=11466286; DOI=10.1128/JB.183.16.4823-4838.2001;
RA Nolling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA Doucette-Stamm L., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA Smith D.R.;
RT "Genome sequence and comparative analysis of the solvent-producing
RT bacterium Clostridium acetobutylicum.";
RL J. Bacteriol. 183:4823-4838(2001).
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
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DR EMBL; AE001437; AAK80069.1; -; Genomic_DNA.
DR PIR; B97160; B97160.
DR RefSeq; NP_348729.1; NC_003030.1.
DR RefSeq; WP_010965410.1; NC_003030.1.
DR AlphaFoldDB; Q97HA2; -.
DR STRING; 272562.CA_C2111; -.
DR GeneID; 44998592; -.
DR KEGG; cac:CA_C2111; -.
DR PATRIC; fig|272562.8.peg.2313; -.
DR eggNOG; COG1293; Bacteria.
DR HOGENOM; CLU_022481_2_1_9; -.
DR OrthoDB; 9766163at2; -.
DR Proteomes; UP000000814; Chromosome.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|HAMAP-Rule:MF_00844};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000000814};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 457..550
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
FT COILED 378..405
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
SQ SEQUENCE 570 AA; 66398 MW; A5B85223FC9D2748 CRC64;
MALDGIYIHS ILHELKDKLL GGRISKVNQP EKDEINLTIR TTSNSNIKLL ISANPTYPKI
HITDLIKTNP IKAPMFCMVL RKHLLNSRIL KIDQIESDRI VIIDFETTDE LGFNSIYKLY
IEIMGRHSNI SLVREHDNTI MDCIKHITPE VNRYRTLLPG CEFVLPPKSD KLNPFNFEYN
DLKDYISEKD ITKNPNMFSK TFTGVSSTLS KELYFNVEKL NSLEAAFDYL KTIFDKVKNN
DFYFASYLTN GYVKDFYCLR LNYLEGFSEK TYATPSLLIQ EFYFEKDKAE RLKNRSSDLQ
KIVNTNLERC RKKNEIYEDE LNECANKDTY KIYGELLTSN IYTIKKGDKT ATVLNYYSEG
NESITIKLDE NKTPSENIQR YFKKYNKLKK TEENAQIQIK NNNEEIEYLQ SVLTNIENSD
TYSDIEDIRQ ELIDTEYIRF SKKNKQKKKD KISKPMHIKS KDGIDMYIGK NNKQNDYLTL
KFADKRDIWF HTKNIPGSHI IVKNFGDIPE TTLIEAASIA AYYSKARESS NVAVDYTEVK
NVRKPSGAKP GMVIYYTNKT IYTNPKEPIT
//
